Porcine intestinal organoids are 3D cultures derived from porcine stem cells or porcine intestinal crypts containing stem cells, which can self-renew and differentiate into tissues similar to those in vivo. Due to the high structural and physiological similarity between pigs and humans, porcine intestinal organoids can not only meet the needs of agricultural production and ecological environment research, but also serve as an in vitro model for human drug screening and toxicology studies. Compared with other in vitro models, porcine intestinal organoids have the advantages of being similar to in vivo tissues, stable phenotypes and genetic properties. Porcine intestinal organoids show great potential in simulating host-microbial interactions, drug screening and nutritional development studies, but their construction still lacks a fixed process, which affects experimental repeatability. In this review, we will review culture of porcine intestinal organoids, the exposure method of apical villi, the interactions between microorganisms and hosts, and the current challenges.