As an important and fundamental resource of scientific research, laboratory animals have become essential tools for the continuous advancements in life sciences, medical research, drug development, and other fields. With the development of related laws and regulations, the welfare of laboratory animals is increasingly valued by the general public and international research communities alike. To ensure the welfare of laboratory animals, the ethical review and conduct of laboratory animal practitioners should be standardized, incorporating and adapting advanced international practices with those in China. This article primarily outlines the process for reviewing and approving animal use protocols, along with the standards for evaluation, with the aim of providing a reference for researchers writing animal use protocols and for International Animal Care and Use Committee members conducting ethical reviews of laboratory animal welfare.