Objective To explore the effects of a Chinese medicine formula on regulating glucose, lipid, and cytokines in ZDF rats by transforming Qi and eliminating turbidity. Methods ZDF ( fa / fa) rats were divided randomly into diabetic (DM) and Chinese medicine-treated (TCM) groups (n= 8 per group) according to the levels of glucose after feeding with a high-fat diet. ZDF ( fa / +) rats were used as a control group ( NC, n= 8).“Qihuatongtiao formula”, which functions by transforming Qi and eliminating turbidity, was administered to rats in the TCM group for 6 weeks. Blood glucose levels were monitored and an oral glucose tolerance test was performed in the last week. Six weeks later, samples were collected and serum was retained for the detection of four lipid items and free fatty acids. Insulin levels were detected by ELISA. Pathological changes in the pancreas were observed by HE staining. Cytokine levels were measured using RayBio cytokine arrays. Results Compared with the NC group, the levels of blood glucose, four items of blood lipid, free fatty acid, insulin, and inflammatory cytokines (CXCL3, IL-2,IL-1α, etc. ) in the DM group were significantly increased, and the levels of blood glucose, four items of blood lipid,free fatty acid, insulin, and inflammatory cytokines in the TCM group were lower than those in the DM group.Degranulated islets were observed in the pancreas of rats in the DM group, while fewer degranulated islets were present in the TCM group. Conclusions The Chinese medicine “Qihuatongtiao formula”, which functions by transforming Qi and eliminating turbidity, could regulate glucose and lipids and alleviate inflammatory lesions in ZDF rats.