Objective To establish a germ-free Chinese hamster model using cesarean section-based purification techniques. Methods Female SPF Chinese hamsters were mated with males at a 1 ∶ 1 ratio, after which the males were separated following copulation. Surrogate mother rats were introduced into the experimental environment 1 ~ 2 d prior to the donor females. At term, pregnant Chinese hamsters underwent hysterectomy under a super-clean workbench and were immediately transferred to isolation units for purification. The uteri were dissected to obtain neonatal pups, which were subsequently fostered by germ-free ( GF) grade ICR mice. After successful fostering, the pups were transferred to isolation units for continued rearing. Sterility of the isolation units was monitored monthly. Results Two cesarean sections were performed. The first fostering attempt was unsuccessful,whereas the second was successful, yielding nine pups with a survival rate of 56%. All GF-grade Chinese hamsters met the requirements of GB/ T 14926. 41-2001. Conclusions Using GF-grade ICR mice as foster mothers in combination with cesarean section purification technology, microbial decontamination was successfully achieved, Resultsing in the establishment of a of Chinese hamster model.