[Abstract] Objective Visfatin is an adipokine associated with insulin resistance and type 2 diabetes (T2D). But the effects are controversial in published articles. The aim of the present study was to determine the phenotypic characteristics of Tg-visfatin×ob/ob mice and explore the effect of visfatin by Tg-visfatin mice and type 2 diabete mice ob/ob. Methods Tg-visfatin mice was crossed with ob（+/-）mice to produce homozygotes Tg-visfatin×ob/ob mice and ob/ob mice. The body weight of two kinds of mice were recorded from 1 to 9 months of age. The Intraperitoneal glucose tolerance test(IPGTT) and insulin tolerance test(ITT) were determined at 3, 5, 9-months-old mice, and the death rate of two kinds of mice were recorded from 9 to 11 month old. Results The body weight from 1 to 9 months of age was almost the same. From the result of IPGTT and ITT, compared with ob/ob mice, at 3 months of age, the glucose tolerance and insulin tolerance of Tg-visfatin×ob/ob mice was reduced; at 5 months of age, only glucose tolerance of Tg-visfatin×ob/ob mice was reduced; when at 9 months of age, the glucose tolerance and insulin tolerance of Tg-visfatin×ob/ob mice was aggravated. The death rate of Tg-visfatin×ob/ob mice was improved by 44.4% than ob/ob mice from 9 to 11 months old . Conclusions The results demonstrate that Visfatin plays a positive role in attenuating glucose tolerance and insulin tolerance of ob/ob mice at 3 and 5 month old, but with the growth of age, visfatin aggravate glucose tolerance and insulin tolerance of ob/ob mice at 9 month old and the death rate improves from 9 to 11 month old.