【Abstract】Objective To explore the animal model of syndrome used in the study of hot flash phenomenon in women. Methods Twenty-four female SD rats were randomly divided into three groups: sham operation group, bilateral oophorectomy group and tamoxifen group, with 8 rats in each group. Two kinds of hot flash animal models were induced by bilateral oophorectomy and tamoxifen intragastory at 10 mg/kg/d, respectively. On the 14th and 28th days of modeling, the open field activity, anal temperature and body surface infrared thermogram of rats were detected. On the 29th day, the rats were killed, and the uterus was weighed and pathological sections were made. Blood estradiol and epinephrine were determined by ELISA. The gene expressions of adrenal sex hormone synthetase (Star, Cyp11a1, Cyp17a1, Cyp19a1, Por, Hsd3b2, Hsd17b1) and catecholamine substance synthetase (Th, Ddc, Dbh, Pnmt) in adrenal medulla were detected by RT-qPCR. Results Compared with sham operation group, the body weight of rats in bilateral ovariectomy group increased significantly (P < 0.01), while the body weight of tamoxifen group increased slowly. In the bilateral ovariectomies group, the maximum body surface temperature was significantly decreased on day 28 (P < 0.01), the difference between the maximum temperature and the minimum temperature in the abdomen was significantly increased on day 14 (P < 0.05), the difference between the maximum temperature and the minimum temperature in the back was significantly increased on day 28 (P < 0.01), and the open field activity of the rats was decreased (P < 0.01). The maximum body surface temperature of the tamoxifen group was significantly decreased (P < 0.01), but the open field activity of the rats was increased (P < 0.01). The uterine index of rats decreased significantly in both models (P < 0.01). Compared with sham operation group, E2 in bilateral oophorectomy group and tamoxifen group was significantly decreased (P < 0.01), NE and Epi were significantly decreased (P < 0.05), β-EP in bilateral oophorectomy group was also significantly decreased (P < 0.05). Compared with sham operation group, the adrenal Cyp11a1 gene expression in bilateral ovariectomized rats was significantly increased (P < 0.05), while the Cyp17a1 and Hsd17b1 gene expressions were significantly decreased (P < 0.05). The gene expressions of Star and Por in tamoxifen group were significantly increased (P < 0.01), while the gene expression of Cyp17a1 was significantly decreased (P < 0.01). Pnmt gene expression was significantly down-regulated in bilateral oophorectomy group (P < 0.01). conclusion Bilateral ovariectomized rats can be used for the study of perimenopausal hot flashes, whose syndromes are similar to those of kidney Yang deficiency and Yin deficiency in traditional Chinese medicine.