【Abstract】 Objective Through the modified bilateral carotid artery ligation way to establish VD rat model to observe the changes of cerebral blood flow and the expression of angiogenic proteins. Methods 36 Sprague-Dawley male rats were randomized into sham group（n=18）and model group（n=18）. In the sham group, only the bilateral carotid artery was isolated without ligation, while in the model group, bilateral carotid artery were ligated to establish VD model. Morris water maze behavior test was applied before and 14 days after modeling, the variation of cerebral blood flow was detected by laser speckle contrast imaging. The protein expressions of HIF-1α, VEGF and HO-1 were detected by Western Blotting and the contents of IL-4 and IL-10 were detected by ELISA. Results 14 days after modeling,the escape latency was significantly prolonged and the frequency of crossing the platform had significant decrease for model group as compared with the sham group (P < 0.05). 2 hours,3 days,7 days after modeling, the cerebral blood flow of the model group was significantly lower than that of the sham group (P < 0.05). 14 and 21 days after modeling, there was no significant difference in cerebral blood flow between the sham group and model group (P > 0.05).In the model group, cerebral blood flow decreased to a minimum at 2 hours after modeling (P < 0.05), and then began to recover. The peak of recovery occurred at 3 to 7 days after modeling, and returned to the level before modeling on the 14th day after modeling.At day 21 postoperatively, the expression of HIF-1α, VEGF and HO-1 proteins in hippocampus of the model group were increased remarkably (P < 0.05)and the contents of IL-4 and IL-10 in serum of the model group were significantly rose in comparison with sham group(P < 0.05). Conclusion The variation of cerebral blood flow in VD rats model establish by the modified bilateral carotid artery ligation way showed a certain time rule.At day 21 postoperatively, HIF-1α, VEGF and HO-1 in hippocampus increased significantly, accompanied by increased levels IL-4 and IL-10.