Objective Through single factor modeling method and multi factor combined modeling method, to prepare the rat models of liver stagnation syndrome constipation type irritable bowel syndrome (IBS-C), and provide ideal experimental animal models of IBS-C by comparing different indicators. Methods 42 sprague-dawley rats were randomly divided into blank group (Normal group), cold water gavage group (Cold group), restraint group (Restrain group), tail clipping group (Tail group), cold water gavage+restraint group (C+R group), and cold water gavage+tail clipping group (C+T group). During the modeling period, the body weight, food intake, water intake, and survival status of each group of rats, and open field behavior, fecal Bristol score, visceral sensitivity, and small intestine propulsion were observed. HE staining was used to observe the pathological changes of the rat colon, and ELISA method was used to detect the content of 5-HT and VIP in serum and colon. Results After modeling, the weight loss of rats in each model group decreased (P < 0.05 or P < 0.01), the amount of food and water decreased, and the content of 5-HT in serum of each model group increased. In the Cold group, the number of fecal particles and Bristol score decreased, while the content of 5-HT in the colon increased (P < 0.05 or P < 0.01); the total distance and average speed of the restraint group in the open field decreased (P < 0.01); the preference for sugar water in the Tail group decreased (P < 0.01); in the C+T group, the preference rate for sugar water, total open field distance, small intestine propulsion rate, defecation particles, and Bristol score decreased, while the content of 5-HT in the colon increased and the VIP content decreased (P < 0.05 or P < 0.01); the total distance, average speed, and VIP content in the colon of the C+R group decreased (P < 0.05). Except for the Tail group, all other model groups showed visceral hypersensitivity (P < 0.05 or P < 0.01) at various pressure values on the 7th and 14th day of modeling; pathological observation showed that no significant inflammatory cell infiltration or pathological changes were observed in each model group. Conclusions The combination of ice water gastric lavage and tail clamping method can successfully establish a rat model of liver depression syndrome in IBS-C, which maybe the first choice of five methods and lays the foundation for systematic and in-depth research on the mechanism of traditional Chinese medicine in preventing and treating IBS-C.