Objective To explore the effect of Qinji Liangxue mixture (QLM) on similar animal models of hemorrhoid. Methods Animal models of acute hemorrhoid in rats were made by using croton oil preparation (COP) and glacial acetic acid respectively. Eighty SD female rats were randomly divided into control group, model group, low-dose QLM group (QLM-L), high-dose QLM group (QLM-H) and diosmin group (DOSM), with 8 rats in each group. Except for the control group, all the other groups were treated with COP or glacial acetic acid, and then treated with different drugs for 7 days. HE staining was used to observe the anorectal histomorphology induced by COP and glacial acetic acid, and ELISA was used to detect the levels of IL-8 and TNF-α in serum of rats induced by COP, and the area of perianal ulcer induced by glacial acetic acid was recorded. Twenty-four C57 mice were randomly divided into control group, QLM-L group, QLM-H group and adrenal chromazone tablet group (CT), with 6 mice in each group for 7 days. The hemostatic effect of QLM was evaluated by capillary method and tail cutting method. Results QLM can significantly improve the pathological injury of animal models with similar hemorrhoid diseases, reduce the score of pathological changes induced by COP (P < 0.05), reduce the levels of serum IL-8 and TNF-α(P < 0.05),, reduce the area of perianal ulcer induced by glacial acetic acid (P < 0.05),, and shorten the time of coagulation and bleeding(P < 0.05). Conclusions QLM has good anti-hemorrhoid activity, which may be achieved by anti-inflammation, hemostasis and reducing tissue damage.