Objective To assess the sweat secretion of Yin-deficiency ovariectomized rats and to investigate the changes of biochemical indexes. Methods Eighteen SD female rats were randomly divided into sham operation group, model group, and positive control group with six rats in each group. The sham operation group were taken sham operation, the model group and the positive control group underwent bilateral ovary removal surgery, and L-thyroxine (92mg·kg-1) was given once a day from the 7th day after the operation for 7 consecutive days to establish Yin-deficiency ovariectomized model. The positive control group was orally administered Qinggu San Tang (7.3g·kg-1) daily, while the sham operation group and model group were orally administered an equal amount of distilled water once a day for a total of 14 days. Sweat secretion from the rat foot-plantar region was determined using the Wada-Takagaki reagent colouring method. At the end of the experiment,blood was taken from the abdominal aorta, and the tissue of the paw pads of rats were separated. Serum cAMP, cGMP, LH, GnRH, and E2 levels were measured by enzyme-linked immunosorbent assay (ELISA). Western blot was used to determine the expression levels of M3 receptor, β2 receptor, and aquaporin-5(AQP5) in the paw pad. Results (1) Compared with the sham operation group, rats in the model group were more irritable and aggressive, their body weights decreased while their average temperature increased significantly, sweat secretion increased significantly; (2) Serum cAMP and cAMP/cGMP ratio increased, LH and GnRH increased significantly, and E2 level decreased; (3) M3 receptor expression was down-regulated and β2 receptor, aquaporin (AQP5) expression up-regulated in paw pads of rats. After 2 weeks of positive control treatment, serum cAMP and the ratio of cAMP/cGMP decreased significantly, LH and GnRH levels decreased, but no statistical difference was observed for serum E2 level. The expression levels of M3 receptor, β2 receptor, and aquaporin (AQP5) were down-regulated in paw pads of rats. Conclusion: Sweat secretion was significantly increased in the perimenopausal syndrome kidney yin deficiency "combined disease and evidence" model established by desiccation combined with thyroxine, and the mechanism may be related to the changes of cGMP, cAMP, and its key proteins M3R, β2AR, and AQP5 in sweat glands that regulate sweat secretion.