Objective Compare the serological and pathological characteristics of three nonalcoholic steatohepatitis (NASH) models: high fat diet (HFD) with carbon tetrachloride (CCl4) injection, methionine and choline deficient diet (MCD) and Aymlin liver NASH (AMLN) diet induced NASH models, and make primary study on their pathological mechanisms. Methods Three NASH models were established by feeding HFD with CCl4 injection for 10 weeks, MCD for 8 weeks and Amylin diet for 26 weeks. After feeding, serum transaminase (ALT, AST) and glucose (GLU), liver triglyceride (TG), total cholesterol (TC), Superoxide dismutase (SOD) and Malondialdehyde (MDA) levels were measured. Insulin level was measured by ELISA and HOMA-IR index was calculated. Hematoxylin-eosin (HE) staining, Sirius red staining and Oil Red staining were used to indicate the pathological changes of liver. Furthermore, NAS score was used to evaluate the pathology grade. Results Compared to normal control mice, all mice in three model groups had obvious increase in serum transaminase, liver TG, TC, SOD, MDA level. The level of serum FINS, GLU and HOMA-IR index were significantly increased in mice of Amylin and HFD with CCl4 group, but decreased in mice of MCD group. According to HE staining, Oil Red staining and NAS score, all mice in three groups MCD mice had NASH phenotype changes. Liver collagen deposition was most obvious in mice of HFD with CCl4 group. Meanwhile, liver lipid droplets were most abundant in mice of AMLN group. Conclusions All three NASH models show different advantages and disadvantages. NASH models induced by Amylin diet recaptures the key NASH features observed in patients.