Objective To investigate the differences in blood pressure phenotype, renal pathological changes and related pathogenic pathways in genetically diverse hypertensive mice from 13 strains. Methods The mice with different genotypes were obtained by crossing 13 genetically diverse strains with Cckbr-/- mice, and then the blood pressure was measured by BP-2000 noninvasive blood pressure analysis system, the expression of CCKBR protein in mice kidney was detected by Western blot, the pathological changes of mice kidney tissue were detected by HE staining and immunohistochemistry (IHC), and the pathogenic pathways related to essential hypertension were screened by RNA-Seq. Results In 3 specific mouse strains (A/J, LOT, FIM), the systolic blood pressure (SBP) of Cckbr-/- group was significantly different from that of Cckbr+/+ group. HE staining and immunohistochemistry showed that hypertension caused a certain degree of renal injury in mice. GO and Pathway enrichment analysis showed that differentially expressed genes were enriched in metabolism and circadian rhythm regulation. Conclusion Genetically diverse mice can better simulate the genetic background of the population and provide a new resource for studying the pathogenic genes related to essential hypertension.