Gene knockout technology is increasingly becoming a powerful tool for establishing animal models of hyperuricemia (HUA). HUA gene knockout animal models are not only helpful to reveal the molecular mechanism of uric acid metabolism, but also of great value for evaluating potential therapeutic strategies. In this paper, the application of gene knockout technology in HUA animal models was discussed in detail by reviewing the domestic and foreign literature, focusing on urate oxidase (UOX), glucose transporter 9 (GLUT9) and ATP-binding cassette transporter G2 (ABCG2) were knocked out in experimental animals in order to provide reference and guidance for the further establishment of HUA animal models by gene knockout technology.