Abstract: Objective To investigate the establishment and evaluation of intratracheal infusion of bleomycin with different concentrations on the induced idiopathic pulmonary fibrosis (IPF) mouse model. Methods Male C57BL/6J mice were randomly divided into the control group, Model-L group (1.5mg/kg, BLM), Model-M group (2.5mg/kg, BLM) and Model-H group (3.5mg/kg, BLM). IPF mouse model was constructed by one-time intratracheal infusion of bryomycin. The general status, body weight, survival rate and lung coefficient of mice in different groups were compared, and pathological changes of lung tissue, hydroxyproline content ,fibrotic markers and inflammatory factors were observed. Results Compared with the control group, the survival rate of the low, medium, and high dose groups in the model decreased, and body weight showed a downward trend (P<0.001), with a significant increase in lung coefficient. Additionally, inflammatory infiltration (P<0.01) and collagen deposition (P<0.0001) were exhibited in the lung tissues of the low, medium, and high dose groups. Hydroxyproline levels in lung tissue and serum were significantly elevated (P<0.05). The expression of fibrotic markers α-SMA, FN, COL1A1 at both mRNA and protein levels was upregulated, and the mRNA expression of the inflammatory factor TGF-β1 increased. Conclusion 1.5, 2.5 and 3.5mg/kg bleomycin can induce IPF model of C57BL/6J mice. Combined with the results of survival rate, body weight and lung coefficient changes, lung tissue gross and pathological changes, fibrosis related biomarkers, 2.5mg/kg bleomycin is the optimal concentration for IPF induced mouse model.