Sarcopenia is an age-related skeletal muscle degenerative disease. The physiological aging mice are the most commonly used animal model for studying sarcopenia. Since sarcopenia is characterized by decreased skeletal muscle mass and reduced muscle strength, the exercise performance is a reflection of muscle function and widely used to evaluate sarcopenia. The motor function evaluation for sarcopenia mice model is generally designed based on muscle endurance, muscle strength, coordination and balance. The methods include run table exhaustion, voluntary wheel, grip strength, the horse grid, bars and balance beam test, etc. By collating recent publications, we systematically summarized the methods for motor function evaluation, including the test principle, test procedure, evaluation indexes, advantages and disadvantages, and then proposed the operational program for evaluation of sarcopenia phenotype, which will be of help for researchers to choose appropriate evaluation methods according to their specific research purposes. Furthermore, the innovative technology of motor function assessment was summarized, which would be instructive in evaluation of skeletal muscle function and diagnosis of sarcopenia.