Abstract:Objective: To develop an animal model that can replicate the clinical phenotype of severe asthma.Methods: Ovalbumin (OVA) combined with interleukin (IL)-33 or varying doses of lipopolysaccharides (LPS) were used to explore the construction of a severe asthma mouse model. Following model establishment, lung function, the number of inflammatory cells, and lung tissue pathology were assessed to evaluate the model. Key genes associated with severe asthma were identified from the GEO database and validated in the constructed model.Results: OVA combined with IL-33 or LPS (5 μg) significantly increased airway resistance, the number of inflammatory cells in bronchoalveolar lavage fluid, and aggravated lung tissue pathological damage compared to the OVA asthma model. In the constructed severe asthma model, the expression of key genes was consistent with those observed in clinical severe asthma patients.Conclusion: OVA combined with IL-33 or LPS (5 μg) can serve as an experimental animal model for severe asthma.