Objective To evaluate the physical protective effect of carbomer labor gel on the birth channel in-vitro and ex-vivo experiments by constructing a simulated delivery model in SD rats and Beagles in the form of inserting a catheter balloon into the birth channel and applying external force. Methods In in vivo study, the SD rats were divided into blank control group, medical liquid paraffin group, negative control group and carbomer obstetric gel low and high dose groups. In the in vitro experiment, SD rats, as well as Beagle dogs in both in vivo and in vitro experiments, were divided into blank control group, medical liquid paraffin group, carbomer obstetric gel low dose and high dose groups. The blank control groups and negative control group were not administered any treatment, the medical liquid paraffin groups were given medical liquid paraffin, and the carbomer obstetric gel low and high dose groups were given different doses of gel. In the in vivo test, catheters were inserted into the birth canal of all animals after drug administration. Except for the blank control group, a certain volume of normal saline was injected into the catheter balloon of the other groups, and external force was gradually applied until the balloon just came out of the body. Hematoxylin-eosin (HE) staining was used to detect the extent of impairment of the birth canal tissue. In the in vivo test, ex vivo birth canals were used to investigate the maximum percentage of smooth muscle stretchability. Results Compared with the medical liquid paraffin group, the external force required for balloon release in the birth canal was significantly lower in the low and high dose groups of carbomer birth canal gel in SD rats and Beagles (P < 0.05), and there was a concentration-dependent reduction in the degree of injury and the incidence of lesions in the low and high groups of carbomer birth gel in the birth canal of Beagles, which was significantly less severe in the site of administration in the high dose group of carbomer birth gel in SD rats and no significant difference in site lesions was observed in the other three groups of carbomer birth gel administered to the SD rats. No significant difference in site lesions was observed. Compared with the medical liquid paraffin group, the carbomer birth canal gel was effective and dose-dependent in increasing the extensibility of isolated smooth muscle. Conclusion Successfully constructed simulated delivery models for SD rats and Beagles; Carbomer obstetric gel demonstrated labor facilitation during the delivery process in SD rats and Beagle dogs, with better efficacy than medical liquid paraffin.