Abstract:本文报告广西猕猴新品种GMm-Ns的两个家系(?)代,(?)4只猕猴颅的测量数值;两个家系F_2代两只猴丘脑内侧背核(MD)立体定位数值。非本品种猴颅、脑测量数值作对照。结果表明实验组颅型的遗传性以及脑内结构立体定位数值十分稳定。G-O_p/APO-O_p能作为种培殖脑研究专用实验猕猴及其质量监测指标。

Abstract:Genetic polymorphism of two plasma proteins prealbumin ( PA ) and transferrin ( Tf ) , and two plasma isozymes, malate dehydrogenase ( MDH) and alcohol dehydrogenase ( ADH ) from 118 laboratory-bred Rhesus monkeys ( M.mulatta ) was analysed by thin-layer polyacry lamide gel electrophoresis.The result suggested that ADH showed the monomorphism, the PA.Tf and MDH showed the polymorphism.The electrophoric pattern of PA can be divided into 7 phenotyp s, that is AA, AB, AC, AD and BB, CC. EE. The genes frequencies of A, B, C, D and E were 0.343, 0.072, 0.042, 0.009 and 0.081 respectively .The Tf can be divided into 17 phenotypes, that is CC, DD, EE, FF, GG, CD, CE, CG, CH, DE, DF, DG, EF, EG, EH, FG.The genes frequencies of C, D, E, F, G and H were 0.064, 0.38, 0.133, 0.111, 0.244, and 0.014 respectively. The MDH isozyme can be divided into MDH1-1 and MDH2-1 two phenotypes MDH1 and MDH2 frequencies were 0.953 and 0.042. The experimen tal results were compared to those of previously reported experiments.

Abstract:对13头成年母猴,用黄体酮 前列腺索(PGF_(2a))处理,诱发月经周期,并用三种不同的处理方案,注射外激性促性腺激素诱发等超数排卵。对171个大滤泡(直径≥4mm)实行外科手术采卵,滤泡穿刺吸卵共获147个卵细胞,平均每头猴采卵11.31±6.47个,卵细胞回收率为85.96％。精液用直肠电刺激法采集,精子经TALP培养基洗涤二次,并在含10％灭活猴血清的TALP液中,加入1mM CAMP和lmM咖啡因,预孵育6～3h。卵细胞在含10％灭活猴血清的TALP或Ham'sF-10培养基中作体外成熟的培养,经约4～12h孵育,卵细胞体外成热率为28.57％。成熟的卵细胞(n=42,中期Ⅱ)和已预孵育的精子一起12～24h,并有部分在前次输精后5～12h再次输精,在42～56h后有9个受精卵继续发育至2细胞期和桑椹期阶段,体外受精率为35.71％(15/42)。

Abstract:2月龄的Wistar大鼠,经颈静脉注射~3H—Ru486(~3H—R)或~3H—孕酮(~3H—P)后,可见自显影银粒在子宫肌层细胞核内有明显的聚集,而腔上皮和腺上皮内银粒分布相对较少。在阴道、输卵管肌层细胞核内也有聚集。垂体前叶细胞的核、浆以及下丘脑、视上核、室旁核、弓状核等核团内神经元的胞核内都有相似的银粒聚集。所有动物的隔肌内均未见自显影银粒定位。用非标记Ru486,孕酮或地塞米松竞争的结果表明,Ru486在下匠脑、垂体、子宫等水平均和孕酮竞争特异性结合部位,很可能是孕酮受体结合部位。这提示Ru486在体情况下对上述各个水平的生理机能均可能产生影响,其抗早孕和诱导月经可能是通过多个环节而起作用的。

Abstract:线粒体DNA的限制性内切酶图谱在不同的种系及亚种间存在多态性。我们分别用五种限制性内切酶BamHⅠ、EcoRⅡ、HidⅢ、PstⅠ、MspⅠ对60只中国KM小鼠(雌雄各半)的线粒体DNA进行了酶切电泳分析,结果未发现多态性,且这五种限制性内切酶图谱均与BALB/c小鼠相同。这表明中国KM小鼠与绝大多数实验小鼠一样,均起源于欧州的野鼠M.m.domesticu。未见到KM小鼠经其它亚种野鼠母系遗传污染的迹象。

Abstract:In this study, we analyzed Q-H bands ( combined fluorescene staining with Quinacrine Mustard and Hoechst 33258, H-bands equals C-bands ) of ten inbred strains mice.Because of C-bands polymorpholism and genetic stability, chromosomes within one cell hava been arranged and grouped according to the size of their C-bands .Therefore, the characteristic chromosomal C-band markers for each of ten strains were made to be the cytogenetic monitoring index .Meanwhile ,the comparative results of biochemical markers with chromosome markers show that in this way, chromosomal C-band markers, which as the terminal markers in mouse chromosome map, have greatly extended the genetic m itoring zone and approached the, ideal model of genetic monitoring in inbred mice.

Abstract:将树鼩胸主动脉分层剥离,用组织块贴壁法,体外培养出主动脉内皮细胞,历经一年,传至23代,命名为TSaec-8910。倒置显微镜下细胞单层生长,呈铺路石样镶嵌排列,第Ⅷ因子相关抗原阳性,透射电镜观察,细胞质内未找到Weibel-Palade小体。细胞生长曲线及分裂指数示9～12d汇合成单层,按1:2或1:3传代,传代间隔为lO～14d,细胞冻存复苏后接种存活率为31.5％,细胞染色体检查为二倍体细胞,2n=62,雄性。内皮细胞生长因子、上皮细胞生长因子、条件培养基和附着底物对TSaec-8910细胞有明显影响,细胞在玻璃瓶壁上贴壁时间为24～18h,而在涂有鼠尾胶瓶壁则为4h左右,内皮细胞生长因子、上皮细胞生长因子能促进TSaec-8910细胞贴壁和增殖,20％TSaec-8910细胞条件培养基亦能良好地维持细胞形态。

Abstract:T he breeding, reproduction and care for infants of the saddle back tamarin (Saguinus fuscicollis) in captivity have been studied. The young tamarin has been grown up in the laboratory at first in China. The above resuits indicated that successful reproduction and growth of young tamarins at suitable temperature, relative humidity, nutriment, fluorescent light mimicking the full daylight spectrum, and better care are necessary.

Abstract:An adult male, laboratory breeding Macaca nigra suffered hepatic cellular carcinoma was reported. It was discussed the relationship among the spontaneous diabetes, retrovirus D infection and hepatic cellular carcinoma.