Abstract:iven the stronbv association of Apolipoprotein E (ApoE )wiih Athe-rosclerosis (AS) and Alzheimer's Disease (AD), this project aims ofsetting upthe transgenicmice mice a mutant human ApoE gene. Thisspecial animal model would be great benefit to understandinato the cffect ofabnormal ApoE gene expression on lipids metabolism and its roles in thepalhogenesis of AS and AD.A 6.0kb DNA fragment from the recombinant piasmid PME7 containingthe mouse MT-I promoter and the human ApoE7 (Glu 244, 245-Lys 244,245) genomic DNA was recovered from low melting agarose electophoresisand purified by CsCI ultracentr ifugation.Then it was microinjected intothe male pronucleus of the fertilized eggs of KM mice. 441 eggs survivedfroni microinjection were transplanted into the oviduct of 20 pseudopregn-ant feiiiaie, 15 pseudopregnant nivice got pregnant and gave birth to 80pups. Genomic DNA were extracted from the tails of the pups Dot Blothybrid ization was first used for screening. Then Southern Blot hybridi-zation was used fordetermination of the integrationof h-ApoE, gene. A2. Okb DNA fragment including the forth exon of human ApoE7 genomicDNA was used as the probe. 2 mice wiare identified of the intergrationof h-ApoE7 gene with 2 and 5 copies respectively. The 2 founder femalstransgenic mice ( Fo generation ) were bred to ordinary KM male mice toset up Fi generation. One mouse aborted on the 17th day of pregnancy.while another one had successfully given birth to 10 pups which are nowready for identification.

Abstract:n this study, we bred 17, 21, 25 lines of HBV transgenic mice by abreeding methodsim ilar to Sing le-founder Breeding. From the resultsof integration and repl icat ion detccted by Polymerase Chain Reaction(PCR)and Southern blotting, we learnt that HBV gene was transmitted steadilyfrom G0 to F5. Moreover, there was always HBV DNA of integrated inicein the serum. Serum HBV DNA was detectable in a high ratio, F1was 94% (102/103) , F2 F3 was 95.3% (61/64) , respectively. So, we canexactlyscreen transgenic mice from the offspring in the light of serumHBV DNA.

Abstract:he plateau pika (Ochotona curzoniae) is a spec ies of newly-developedlaboratory animals. The fundamenta1 gastrointe stinal characteristics of thepika was analyzed and discussed, and the nutritive elements of wild plantsfor the pika being fond of were also analyzed in this paper. Accordingto the result from feeding-test, the basic standard of nutritive elementsfor the pika was about 14.90% raw protein, 25.79% raw cellulose.3.24% fat and 8.49% minerals. This project also indicated that the pikaobviously required more plant cellulose than domesticated rabbit

Abstract:提取四种近交系小鼠的肝脏ＤＮＡ，分别用ＨｉｎｆＩ和Ｈａｅ　Ⅲ酶切。用３３．１５和本课题组克隆的小鼠小卫星阳性克隆ＤＮＡ片断作探针，用［α－３２Ｐ］ｄＣＴＰ随机引物标记，Ｓｏｕｔｈｅｒｎ杂交，获得了理想的小鼠ＤＮＡ指纹图谱。初步确定阳性克隆ＤＮＡ片断Ｖ２具有较好的多态性，可作为小鼠基因组小卫星探针，用于实验动物的遗传监测、基因连锁分析和小鼠遗传图谱的研究。

Abstract:ehave developed a gene transfer system for targeting DNA tohepatocytes in vivo using a DNA carrier that takes advantage of ASGP(asiQio-glycoprotein) receptors for hepatocytes. This carrier systemincludes two kinds of funct ional components: one is ASGP whichcan bind ASGP receptors, the other is poly-L-lysine which can bindDNA in a strong electric interaction. Intravenous injection of 32P-DNA-poly-L-lysine-ASOR (asio lo-orosomucoid) or cqual amount of the mix-ture of individual const ituents demonstrated more speciffc target ingin the liver than in the spleen and the kidneys.

Abstract:本文以ＳＨＲ为实验组，ＷＫＹ为对照组，以灌流压为血管舒缩的指标，对其离体肠系膜血管床，初步研究了钙离子的稳定作用，实验结果如下：（１）ＳＨＲ与ＷＫＹ相比，对血管活性物质去甲肾上腺素（ＮＡ）和氯化钾（ＫＣＩ）均表现出较高反应性。（２）在ＮＡ（１．７×１０Ｍ）使血管床收缩的基础上，Ｃａ￣（＋＋）（２０ｍＭ）使之明显舒张，ＳＨＲ的舒张程度小干ＷＫＹ约１８．１７％（Ｐ＜０．０５，ｎ＝６）．（３）在ＫＣＩ（８０ｍＭ）使血管床收缩的基础上，Ｃａ＋＋（４０ｍＭ）使之明显舒张，ＳＨＲ的舒张程度小于ＷＫＹ约１５．０７％（Ｆ＜０．０５，ｎ＝６）。

Abstract:8 male Sprague Dawley rats were fed with 10% (V/V) ethanol fromdrinking water at an average dose of 8.0g/kg body weight per day for 90days. Control rats were given the common diet without ethanol. Theactivities of alcoho1 dehydrogenase (ADH) and glutathione S-transferase(GST )of rat liver were measured at 30, 60 and 90 days of the experi-ment respectively. The results demonstrated that there was a significantdecrease of ADH and GST activities of rat liver in chronic aicoholintake group (P<0.05, and P<0.01) .

Abstract:给ＤＢＡ／２小鼠双前肢足垫皮下注射２×１０Ｐ３８８细胞后，腹腔注入Ｌ－ＩＦＮｒ（脂质体包裹ＩＦＮｒ）或ＩＦＮｒ４，２０，１００×１０４Ｕ／（ｋｇ·ｄ），连续１０ｄ，结果小鼠生存期分别延长１８．３、２９．３、３３．９、２３．９、３１；９、３３．６ｄ。在第１５天循环血中的单核细胞分别相应降低２５．３％、５４．９％，８９．７％和５５．８％、８５．２％、９０．２％；移植瘤引流区腋窝淋巴结肿大呈剂量依赖性缩小。结果表明两种ＩＦＮｒ制剂均有显著的抗白血病细胞Ｐ３８８转移的作用，Ｌ－ＩＦＮｒ的效果优于ＩＦＮｒ。

Abstract:采用注射兔抗大鼠血小板血清（ＡＰＳ）方法，建立了大鼠免疫性血小板减少模型。大鼠腹腔注射１：４稀释的ＡＰＳ（０．７ｍｌ／２００ｇ体重），连续３ｄ，可使血小板数量显著降低，其降低率为８１±９％（ｎ＝１２），且其骨随巨核细胞增生活跃，但注射ＡＰＳ后对血中红细胞数和白细胞数无明显影响．在注射ＡＰＳ的同时，给予大鼠灌胃强的松（１ｍｇ／２００ｇ体重），可抑制ＡＰＳ所致的血小板减少的下降程度，并促进停止注射ＡＰＳ后血小板数的恢复。以上结果表明，该模型符合免疫性血小板减少性紫癜的病理特征。

Abstract:wo inbred strains of female adult mice (C57BL/6, DBA/2) were usedas animal model with different niajor histocompatibility (MHC) in theexper inient. Samples of al logenic cartilage and syngene ic cartilage weregrafted from the thigh muscle of C57BL/6 mice. Immunogenicity of cartilagewas evaluated in vitro by means of analysing antibody-complement dependent cytotoxicity and cell med iated cytotoxicity. These data implied thatallogenic cartilage was associated with a vigorous specific immune res-ponsc, while isogenic bone did not cause significant cytotoxicity response,and have no significant difforence from negative controls. The resultssuggestLcd that C57BL/6 and DBA/2 can be used as animal model foral logenic cartilage graft ing and immunogenic ity stud ies.

Abstract:i Shou Tang was given to mice orally in the doses of 1g. 2g and 4g/kg scperately for 7 days. The activities of immunity and antistress wereexamined. The results showed that the swimming time and the endurancehypoxia in the 4g/kg group taking Yi Shou Tang were much longer than thecontrol group. Yi Shou Tang in the doses of 2g and 4g/kg improved theanticold activity. Morcver, YiShou Tang with the doses of 1g 2g and 4g/kg increased the serum hemolysin andphagocytic index but it had nosignificant effects on the de layed-type hypersensitivity.

Abstract:用马来酰亚胺和５恶唑氮氧自由基硬脂酸分别标记晶状体细胞膜中蛋白质及脂类，以电子自旋共振方法研究在三硝基甲苯、亚硒酸钠，半乳糖、平阳霉素及紫外线等白内障诱发剂作用下，晶状体细胞膜的氧化损伤变化。结果发现当晶状体细胞暴露于空气中时，晶状体细胞膜脂类氧化早于蛋白质氧化；在五种白内障诱发剂作用下，均是如此。这说明白内障的形成首先是由膜中脂类氧化所引起。

Abstract:uman glioma rarely metastasizes outside the cranium but sometumors are highly metastat ic to thebrain. We developed an in-vivo modelinnude mice to study the metastatic mechanism to prevent glioma but allowmelanoma to pass through the blood-brain barrier. The glioma cellssuspension and melanoma cells suspension were injected in internal carotidartery of nude mice respectively.The glioma group resulted in metastatictumors invading the skull without passing the blood-brain barrier into thebrain. In the melanoma group tumor metastasized into the brain throughblood-bra in berrier in all cases.The tumor metastasis outside or inside thebrain is likely due to its ability to emigrate through the blood-brainbarrier. Direct carotid artery injection in nude mice is technically quitedifficuIt, but the animal model is useful to define cellular genetic, andimmunogical factors involving metastatic brain.