Abstract:本文目的是探讨在成年大鼠初级体感皮层（ＳＩ）内进行局限损毁能否引起损毁区周围的代表区重组。在氯胺酮麻醉下用微电极技术测定隐神经代表区（ＳＡＲ）和坐骨神经代表区（ＳＣＲ），然后用铂电极对ＳＡＲ进行选择性电解损毁。三至四周后进行重复测定。结果表明，在１４例所观察的大鼠中，９例在原损毁区以外发现新生的ＳＡＲ，其面积为０．２０±０．０８ｍｍ２。这表明成年大鼠ＳＩ神经元在中枢损伤后具有一定的重组能力。

Abstract:103 Cynomolgus monkeys were tested for the serum antibody of STLV-1 by Gelatin particle agglutination (PA), Immunofluorescence assay (IFA) and Western blot (WB).The results showed that 13 WB-positive sera reacted in the PA and IFA; A positive reaction,however,was detected in 3 and 1 out of 90 WB-negative specimens by PA and IFA.

Abstract:To observe the difference of the susceptibility to Streptobacillus moniliformis (S.m.), we developed an experimental infection with Streptobacillus moniliformis in 6 strains of mice including KM, C57BL/6J, BALB/c,ICR, NIH and DBA. After peritoneal inoculation, only C57BL/6J and KM mice showed the typical clinic signs and pathologic changes. The mobility and mortality after infection with S.m., were 92% and 80% in KM mice, but in C57BL/6J were 80% and 12%. The KM mice showed the severe symptoms and the most of them died during the acute phase after infection. The NIH mice showed 8%, as well as the BALB/c and the DBA mice showed 4% of mobility but none of them died. ICR was not affected after the infection. For the pathological changes, there was a significant difference between KM and C57BL/6J mice. We observed that in Km mice, it is characterized by congestion, cyanosis and swelling in limb and tail, polyarthritis, diarrhea, and hindlimb paralysis. In C57LB/6J, however, abscesses in subcutaneous lymph nodes and the injection place, purulent polyarthritis were the main symptoms. The results demonstrate that not only the susceptibility to S.m. infection but also there climic signs and pathologic changes are different in the 6 strains of mice. Because of the high susceptibility to S.m. it is considered that as a sentinel animal, the KM mice can be used for routine monitoring of S.m. in rat.

Abstract:Transgene was measured by using PCR method in early embryo development of fertilized eggs in this paper. 100%,77.7% and 44.4% retention of foreign gene were achieved in 1,2 and 88 cellstage resprectively. This result showed that transgene was missed in embryo development with time increasing.

Abstract:本文应用抗人Ｔ细胞活化抗原ＣＤ２５和ＰＴＡ１ＭｃＡｂ对恒河猴ＰＢＭＣ进行间接免疫荧光染色和流式细胞仪分析，结果发现，恒河猴静止ＰＢＭＣＣＤ２５和ＰＴＡ１均为阴性，而ＰＨＡ活化后或经连续２个月注射ｒＨｕＴＮＦ－α恒河猴ＰＢＭＣ均表达高比率的ＣＤ２５和ＰＴＡ１阳性细胞。此结果表明，ＰＴＡ１抗原可作为恒河猴活化Ｔ淋巴细胞的一种有用标志，为ＰＴＡ１分子结构和功能研究以及采用恒河猴作为灵长类动物模型，观察细胞免疫功能状态提供了有用的资料。

Abstract:测定比较了２０日龄的封闭群ＦＭＭＵ白化豚鼠与短毛三色豚鼠３５个血液生理生化指标，结果表明：雄性白化豚鼠的钾、葡萄糖、甘油三脂、总蛋白、红细胞总数、红细胞分布宽度明显高于雌性白化豚鼠，其它项目差异不显著；对两种豚鼠的血液成分测定结果比较发现，ＦＭＭＵ白化豚鼠的氯、磷、尿素氮、葡萄糖、淋巴细胞百分数和红细胞分布宽度比三色豚鼠的低，而尿酸、中间细胞总数、血小板总数，平均血小板容积和平均血小板比容比三色豚鼠的高。

Abstract:Objective:This study was designed to purify Major Histocompatibility Complex (MHC) antigen (H 2) that induce the rejection of organ transplantation in mouse.Methods:Triton/tris was used to extract the crude H 2,to which of the anti H 2 antibodies coupled with sepharose 4B was applied to purify H 2 antigen by affinity chromatography.The coupled H 2 Ag was eluted via 0.5%DOC,0.65M NaCl solution.Results:45kd heavy chain and 12kd light chain from purified proteins showed respectively by electrophoresis.Besides,the pure H 2 Ag was found to have serologically and biologically activity,as determined by antibodies rebinding and cytotoxicity inhibition assay.This method should be useful purification of a large quantity of H 2 Ag for the research in transplantation and functional analysis of MHC antigen.

Abstract:本文报告了２０日龄ＦＭＭＵ白化豚鼠１０个主要脏器重量和１０个脏器系数的测定结果。ＦＭＭＵ白化豚鼠按雌雄分组试验，对两组测定结果进行了对照分析，并与对照组同龄三色花豚鼠的测定结果做了比较。结果表明雄性ＦＭＭＵ白化豚鼠肾、脾、肝、心、肺的重量均明显高于雌性ＦＭＭＵ白化豚鼠，雄性白化豚鼠脑的脏器则低于雌性白化豚鼠，其它项目两者差异不显著；ＦＭＭＵ白化豚鼠与三色豚鼠比较结果表明，白化豚鼠除肾上腺、睾丸重量明显高于三色豚鼠外，其余脏器重量及全部脏器系数，相差不显著。

Abstract:对本室培育的封闭群ＦＭＭＵ白化豚鼠的繁殖性能和生长发育情况进行初步观察，结果表明：ＦＭＭＵ白化豚鼠系三色豚鼠的变异株（种），由于严格采用循环交配的封闭群繁育方法，其产仔数、泌乳力、哺乳期增重等繁殖性能和生长发育指标仍维持较高水平，和三色豚鼠相接近。有关繁殖性能指标为：胎间隔８．６７±１７．４／ｄ、窝产仔数２．８９±０．６６／只、初生个体重８６．３９±１０．１７／ｇ，泌乳力４０２．１±４８．３２／ｇ、离乳数为２．６８±０．４１，离乳率为９０．７１±１２．７１％，离乳个体重为１７８．６５±２６．６８／ｇ。３、９、１５、２０日龄豚鼠个体重分别为８８．７６±１０．８１／ｇ、１２３．１８±１２．５５／ｇ、１６３．４１±２１．１３／ｇ、１７５．３６±２２．１７／ｇ；雌性豚鼠个体重分别为９０．３７±１３．７２／ｇ、１３０．７４±２２．１８／ｇ、１６９．７９±２７．８２／ｇ、１７９．９８±２８．１２／ｇ。

Abstract:选用人胚肺为实验材料，研究了十字花科蔬菜提取物ｏｌｔｉｐｒａｚ对人肺癌病变早期细胞恶性转化的阻断作用。将阻断按时间分为ｏｌｔｉｐｒａｚ在ＣＳＣ作用前、后和同时三组，并设ＣＳＣ、ｏｌｔｉｐｒａｚ、ＤＭＳＯ三组对照，将上述６组体外培养处理的人胚肺组织，提取ＤＮＡ转染Ｒａｔ－１细胞，用低血清筛选、作软琼脂培养、裸鼠接种鉴定，结果在ＣＳＣ、ＣＳＣ→ｏｌｔｉｐｒａｚ两组获得恶性转化细胞，ＣＳＣ组恶性度更大。提示ｏｌｔｉｐｒａｚ对ＣＳＣ所致的细胞恶性转化具有一定阻断作用，ｏｌｔｉｐｒａｚ在ＣＳＣ运用前或同时运用效果较好；进一步推断ｏｌｔｉｐｒａｚ对人肺癌变早期具有一定防治作用。

Abstract:采用脾内直接免疫法和杂交瘤技术获得５株杂交瘤细胞。杂交瘤细胞培养上清液和腹水中单克隆抗体效价分别为１∶８～６４和１０－３～１０－４（ＥＬＩＳＡ）。与其他１０种鼠源性病毒和ＢＨＫ２１细胞无非特异性反应。与传统免疫方法相比较，即节省了抗原、缩短了免疫时间，又可避免因免疫抗原致病性强而导致动物死亡的缺点，是一种具有实际应用的免疫方法。

Abstract:The effects of Chinese diet (pellet) for mouse and rat (Institute of laboratory animal science of CAMS) on growth and several physiological parameters was examined in SHN mice in comparison with Japanese commercial pellet (LAB MRBreeder:Nihon nosna Kogyo KK).Some differences were found in body growth,pattern of estrous cycles,normal and preneoplastic mammary gland growth,major organ weights,serum glucose levels and glucose tolerance by using the two kinds of diet.The result indicate that the Chinese diet examined satisfies the fundamental demands for mouse,Spontaneous mammary tumorigensis tended to be higher and some components of urinary excretion were significantly lower fed by Chinese diet than that by Japanese diet.

Abstract:ＤＢＡ／２小鼠最长妊娠期为２４ｄ，最短妊娠期为１９ｄ，平均妊娠期为２０．９ｄ。平均窝产仔数为４．３只，与昆明小鼠平均窝产仔数６．４只比较，差异高度显著（Ｐ＜０．００１）。２４～３６日龄段为生长最迅速时期，成熟日龄为６０ｄ，９０日龄为适配期。１～１０８日龄与平均体重的相关系数为０．９７７，呈强正相关，相关系数ｔ测验的结果为（Ｐ＜０．００１），显示日龄增长与体重变化之间相关高度显著。

Abstract:观察自发性高胆固醇血症近交系ＮＪＳ小鼠的主动脉形态学特征，了解该品系主动脉粥样硬化发病情况。对主动脉作光镜和电镜观察，并通过图像分析仪分析主动脉内膜的粥样硬化病变面积。该品系８～１０月龄小鼠均见阳性病灶，阳性区域主要分布在动脉弓起始部，胸主动脉各分布的升口处，病变区域多数呈点状，少量呈线状分布。显微镜下内膜层内可观察到少数泡沫细胞聚集，电镜下可见内膜泡沫细胞有较多的细胞突起，胞浆丰富，浆内内质网及溶酶体丰富，脂滴大小不一，核形态不规则，浆内有明确肌丝或致密斑。图像分析计算其主动脉粥样硬化病变面积占主动脉面积的比例为２．０４６±０．２５３％。２～３月龄小鼠中均未见明显病变。近义系ＮＪＳ小鼠８月龄后主动脉能发生脂纹期病变，该品系可作为动脉粥样硬化研究的动物模型。