Abstract:采用类系祖建系的方法 ,对HLA -Ⅱ类基因DRα 和DRB1 　 0 4 0 1转基因小鼠模型进行了繁育。通过PCR和Southernblot分子杂交的方法 ,检测小鼠尾组织的DNA样品 ,以确定DR基因的整合和复制情况。结果表明 ,HLA -Ⅱ类基因DR已稳定地遗传至第五代(F5) ;共产仔 32 6只 ,PCR检测出阳性小鼠 95只 ,阳性率为 2 9.1 %。PCR—Southern印迹杂交检测 ,发现 68只仔鼠整合有DR基因 ,总有效率为 2 0 9%。经Nothern杂交和RT -PCR检测HLA -DR基因在脾脏和肾脏中均有表达。

Abstract:Chromanol2 93B( 2 93B)是一种作用于缓慢激活的延迟整流钾电流 (IKs)的新型选择性阻断剂。本实验采用犬离体血液灌流右心房标本 ,观察了 2 93B对心房标本心率及收缩力的影响。结果表明 ,2 93B降低犬离体右心房血液灌流标本的心率和收缩力 ,2 93B的负性频率和肌力作用与M胆碱能受体无关 ,此外 ,2 93B对 β -受体激动剂的正性频率和肌力作用没有影响。上述结果提示 ,在犬右心房组织中 ,2 93B的负性频率和负性肌力作用与M胆碱能受体和 β-受体无关。

Abstract:Objective　To establish six kinds of stable embryonic stem cell culture system. Methods　Mouse embryonic stem cells (ES D3 line) are maintained in following culture systems The primary mouse embryonal fibroblasts (MEF) in serum medium and MEF in serum-free medium as well as SNL (mouse fibrob last subline ) in serum medium all together consist three kinds of feeder layer dependent culture systems. The serum medium and serum-free medium supplemented w ith recombinant murine leukemia inhibitory factor (mLIF) respectively as well as Buffalo Rat Liver (BRL) conditioned medium consist three varied feeder-free lay er culture systems. The ES D3 cells are passaged 10 times in vitro and their clo ne are observed morphologically. In order to identify ES cell pluripotent and un differentiation on the above mentioned systems, we tested the alkaline phosphata se (AKP) staining which is a marker of the undifferentiated cells and the experi ment of ES D3 cells differentiation in nude mice were investigated. Results　On both feeder layer systems and feeder free systems, the typical ES cells clone shape r evealed as follows nested aggregation (clone) with clear edge and smooth surfac e as well as close arrangement within the clone. Strong positive AKP reactive c ells are observed. On the other hand. the teratomas made up with various tissue s are formed in nude mice. Conclusion　Each of the six culture systems can be us ed to promote the growth of ES D3 and maintain their undifferentiation and pluri potentiaty, which lay a solid foundation for the application of ES cells.

Abstract:The pr otein and antigen mapping of 11 isolates of Pasteurlla pn eumotropica from different of breed facilities were analyzed. The results of 5-2 0% and 10-20% SDS-PAGE showed that there are 25-30 protein bands between 14.4-97 .4kD. The 12% SDS-PAGE revealed the major protein bands evident at approximately 31,34 and 42.7kDa in all isolates tested. 6 of which have the same mapping comp are with the reference strain, 5 are lack of 40kDa band. Western bolt analyses i ndicated the major antigen at approximately 17 and 31kDa in all isolates tested (the reference stain included), with the antisera of P.pneumotropica. Same test with mice sera of the naturally infected by P.pneumotropica, the major antigen at approximately 17kDa. 5 isolates tested lacked of 40kDa protein showed 31kDa b ands, and the others revealed 20 and 28kDa bands. This study demonstrated that t he different of the isolates of P.pneumotropica have the similar protein banding patterns. most of them are at the approximately 31, 34 and 42.7kDa. The major antigen are 17 and 31kD protein. Two types of P.pneumotropica could be classif ied according to 40kDa protein and 20, 28kDa antigen. The study could be the bas ic for purifying the diagnostic antigen and monitoring the P.pneumotropica with SDS-PAGE and Western blot.

Abstract:A pasteurella pneumotropica like bacterium was isola ted from K.M mice colony maintained in barrier system by regular surveying, whos e phenotype and genotype have been investigated with tests available in our labo ratory. This isolate can not be distinguished from pasteurella pneumotropic in m orphology and culture characteristics such as growth and nutrition requirements. Critical biochemical tests show that the strain does not agree with the pasteur ella pneumotropic reference, while it is able to agglutinate with antiserum agai nst pasteurella pneumotropica. The chromosomal DNA G+C mol% value of the strain indicates that it can not be excluded from pasteurellae. Comparing systematic bi ochemical results with what R.boot reported in 1994,suggests that this isolate i s more like those of pasteurella sp group rather than the pasteurella pneumotrop ica Jawetz or Hely biotype, further investigation should be made in order to hav e a definite answer.

Abstract:First,we use human hepatic RNA as templ ate and got CD55 cDNA by PT-PCR. Secondly, CD55 cDNA is combined with promoter a nd polyA of human α-globin, then inserted into plasmid pGEM-5zf. At last,we got a transgenic constructs' of pGEM-5zf-α-globin-CD55 cDNA'. The constructs ma y use to found human CD55 transgenic animal model.

Abstract:A 377 Kunming cell line of Rhesus Monkey Kidney (RMK) from Centre for Medical Pr imates was amplified the cDNA fragments of the pol gene by Nested-PCR,which of a natured infected SFV showed typical CPE after cultured for three weeks,sequence s of products position(nt 5989-6343)were determined directly.The result indicate d that the nucleotid sequences in the region had 48 nucleotids difference(10.78% ) between SFV infected cell and SFV-1 in GENE BANK,and there was 45 nucleotides difference(10.11%) compared with SFVmac in the region, the test with VspⅠ showe d aligment.