Abstract:目的观察啮齿类动物胰管分布、走向、开口，并作种间比较，建立胰液采集方法。方法麻醉动物，暴露肝脏和胆囊，手术插管，推注美蓝，染色。结果小鼠胰腺十二指肠部、胰腺胆管部和胰腺胃脾部组织众多小胰管分别不断汇合成后、前两条大胰管，再汇合成一条胰总管。胰总管不开口于十二指肠，而开口于胆总管。大鼠的胰管也开口于胆总管，但有许多胰管开口，其中有前、后两条较大的胰管。豚鼠各叶胰腺小管的汇合成一条大胰腺管，直接开口于十二指肠升部，无其他副管。结论小鼠、大鼠、豚鼠的胰管走向及开口位置均互相不同，采集胰液的插管方式也各异。

Abstract:目的建立幽门螺杆菌致人胃病的小鼠模型.方法用Ⅰ型幽门螺杆菌(Helicobacter pylori,Hp)对三种SPF级小鼠(BALB/c、NIH、 KM)采用循环滴喂攻击等方法处理,从菌体定值、抗体水平、病理变化三个方面分14、39、69、105 d进行测试.结果 Hp可持续定植于各供试小鼠胃粘膜上,并刺激Hp抗体(IgG)维持一较高水平,Hp在小鼠上主要引起以胃粘膜变性坏死为主,伴有淋巴细胞浸润的炎症反应,这为Hp致人胃病的症状相似.结论为Hp的致病机理,治疗药物和疫苗研究提供了良好的小动物模型.

Abstract:Objective To evaluate the safety of Lactacyd in local dermal and vaginal application. Methods We used acute dermal toxicity test, dermal irritation test, acute vaginal toxicity test, vaginal irritation test, and sensitization test. Results The acute dermal toxicity, dermal irritation, acute vaginal toxicity and sensitization of Lactacyd couldn't be observed. The obvious irritation hadn't be seen in low and middle dose, but inflammation, congestion, hemorrhage and ulceration could be found in vaginal mucous membrane in high dose in vaginal irritation test. Conclusion Lactacyd is safe for skin. However, it isn't adequate for vagina at high dose.

Abstract:Objective To study the effect of Acanthopanax Senticosus (APS) on the immune function and the activity of glutathione peroxidase (GSH-PX) and superoxide dismutase (SOD) during 3-MeDAB hepatocarcinogenesis of rats. Methods 46 male SD rats were divided into three groups randomly: control group received the basal diet, 3-MeDAB group received 0.06% 3-MeDAB in the diet for 10 consecutive weeks, then changed to basal diet, APS group received 0.06% 3-MeDAB along with APS (4.5 g/kg diet) daily in food for 10 weeks, then changed to basal diet along with the same dose of APS till the end of experiment. Phagocytic function of leucocyte was detected by chemiluminescence and the activity of GSH-PX and SOD by method of DTNB and xanthine oxidase. Results Peak/103 PMN and integral (CL 60 min) of 3-MeDAB group were higher than that of control group (P<0.05) and APS group (p<0.01). Phagocytic index/109 WBC of 3-MeDAB group was higher than that of APS group (P<0.05). GSH-PX activity in blood of APS group was higher than that of 3-MeDAB group (P<0.05) and was similar to control group. SOD activity in serum of APS group was higher than both control group (p<0.01) and 3-MeDAB group (P<0.05). MDA content in serum of control was lower than both APS group and 3-MeDAB group. Conclusions The effect of APS against cancer and its immunoregulatory function are evidenced through improving the activity of GSH-PX and SOD and depressing the lipid peroxidation and resisting the compensable increase of phagocytic function of leucocyte duing the hepatocarcinogenesis of rats.

Abstract:目的建立一种狼疮样肾炎动物模型，为人类狼疮性肾炎（ＬＮ）实验和临床研究提供基础。方法取雌性亲代ＤＢＡ／２的淋巴细胞分４次经静脉注射雌性子代Ｆ１代杂交鼠（Ｃ５７ＢＬ６× ＤＢＡ２）。注射时间为０，３，７，１０ ｄ。每次注射淋巴细胞数为５０× １０６。结果尿白蛋白于第４次注射后２周开始升高，第１２周达高峰；血抗ｄｓ－ＤＮＡ水平于第４次注射后２周已明显升高，至第４周达高峰，各时点与对照组差异明显。普通光镜（ＰＡＳ、ＰＡＳＭ）示肾小球基底膜增厚，系膜轻度增生，小管间质炎症细胞浸润；免疫荧光示ＩｇＧ沿基底膜呈线状或颗粒状沉积，部分小管基底膜亦可见其沉积；电镜显示膜性肾炎改变，基底膜增宽，内有电子致密物沉积，上皮细胞足交融合。结论本实验所建立狼疮样肾炎动物模型，主要为膜性肾炎。

Abstract:Objective To study the neuropathological features of transgenic model for Alzheimer's disease by pathomorphological and immunohistochemical methods. Methods Tissue mass was taken from the transgenic mice's brain for dehydration and embedding, congo red staining and immunohistochemical staining. Results Immunohistochemistry revealed plaques in pallium, cerebellum and hippocampus. The expression of PS-1 in transgenic mice obviously increased comparing with control. Congo red staining showed amyloid deposition in intercellular. Conclusion The transgenic model's pathomorphological display conform to the Alzhimer's disease and it could be used for further study.

Abstract:目的为对规模化的SPF级实验动物环境设施中的设备进行系统控制与管理.方法利用图形显示自控技术,对设施中24套主要电器设备进行自动控制.结果该系统能够自动采集和监测设施中27个温度、湿度、梯度压差点和17个氨浓度点环境参数数据;设备自动切换、自动报警、运行工况动画显示; 8个区域实现电视监控;管理信息实时发布.结论该自动控制系统设计先进、实用、操作简便、性能稳定、管理高效.

Abstract:目的测定人工饲养条件下104只健康恒河猴的血液学、血液生化正常参考值,并分析不同性别、不同年龄阶段(青幼年组∶3～4岁,成年组∶5～10岁,老年组∶11～25岁)恒河猴的血液学、血液生化值的差异及相互关系.方法应用全自动血液细胞分析仪和全自动血液生化分析仪,测定及分析非麻醉状态下健康恒河猴血液学和血液生化正常值.结果血液学值中较明显低 (P<0.05) 的项目为MED.血液生化值中♀猴比♂猴明显低 (P<0.01)的项目有ALT、ALP、GGT、CHOL、APOA、HDL、LDL、P3+、BUN,较明显低 (P<0.05) 的项目为ALB.较明显高 (P<0.05) 的项目为UA.各年龄组之间的分析如下:血液学值中RDW值为青幼年组比成年组及老年组均明显低(P<0.01),MEDIAN值为成年组比青幼年组较明显高 (P<0.05),血液生化值中青幼年组比成年组及老年组均明显高 (P<0.01)的项目有ALT、ALP、GGT、P3+,青幼年组比成年组明显高(P<0.01)的项目为BUN、ALP、AST、,青幼年组比老年组明显高(P<0.01)的项目为CK、TF;成年组比老年组明显低(P<0.01)的项目为CREAT,较明显低 (P<0.05) 的为TRIG.结论本文建立了人工饲养条件下健康恒河猴血液学和血液生化值的正常指标,并讨论了年龄、性别对其正常指标的影响,可供参考.

Abstract:目的　探讨将维生素Ａ缺乏（ＶＡＤ）胎鼠作为先天性心脏病动物模型的可行性。方法取１１－１９ｄ不同胎龄正常及ＶＡＤ胎鼠心脏经石蜡包埋、切片及 ＨＥ染色观察其发育情况。结果 １．实验组饲料含维生素Ａ（ＶＡ）７μｇ／１００ｇ，经ＶＡＤ饮食喂养后实验组大鼠血清ＶＡ水平明显低于对照组［（０．１６８±０．０５９）μｍｏｌ／Ｌ Ｖｓ（２．１８±０．２３）μｍｏｌ／Ｌ，ｔ＝３２．８８， Ｐ＜０．００１］。 ２．大鼠死亡百分比：饲养于屏障系统的ＶＡＤ大鼠死亡百分比较饲养于开放系统中的要低４．６倍（１０％ Ｖｓ ４５．８３％．ｘ ２＝１６．６４， Ｐ＜０．００１），对照组为０。 ３．实验组大鼠受孕百分比及每只孕鼠产仔数均低于对照组［５８．３３％ Ｖｓ ８１．５％， ｘ ２＝４．３７，Ｐ＜０．０５：（６．９７±２．７９） Ｖｓ（１３ ±１．０５），ｔ＝７．１６， Ｐ＜０．００１］。 ４．经切片观察１１～１５ ｄ胎龄胎鼠实验组心脏出现明显发育延迟的占３６．６７％， １６～１９ ｄ胎龄胎鼠实验组心脏畸形占４１．４３％，血管异常占１８．５７％。结论ＶＡＤ胎鼠可用来作为先天性心脏病动物模型，但需改进饲养环境以减少异常死亡。

Abstract:Objective To analyse the genetic construction of population in Bama miniature pigs. Methods 31 selected single primers were used to amplify genomic DNA of Bama miniature pigs. The amplified fragments were analysed by the RAPD istance package version 1.04 and the similarity index was calculated. Results 275 bands were obtained in which polymorphisic bands were 85, the frenquency of polymorphisic bands were between 0-66.7%. Different individual pig has various bands, but the frenquency of common band was higher. Based on RAPD patters similarity index (F) calculated was 0.928 (0.78-0.96), mean allelic frequency (q) was 0. 732, and minimun heterozygosites (H) was 0.268. Conclusion Bama,miniature pigs reached a higher degree of homogeneity than ordinary bred pigs.

Abstract:Objective To screen the available MHV combinations for preparing detective kit. Methods Five MHVs were used alone and in different combinations for studying the reactions with standard MHV lines by means of ELISA. The specificity and sensitivity were compared. Results 238 semples of conventional mice from different units were detected. The rate of infection was very high (59%-87%). Conclusion MHV-HU79 isolated in Shanghai of China can be used in the diagnostic kit for MHV antibody.

Abstract:目的调查我国恒河猴STLV-1流行病学特征.方法用免疫荧光法和蛋白印迹法对537只野捕恒河猴,365只繁殖猴和44只D型逆转录病毒抗体阳性猴作了STLV-1血清抗体检查.结果 10岁以上恒河猴的STLV-1抗体阳性率(15.5%)高于10岁以下的恒河猴(6.2%);雌猴的STLV-1抗体阳性率(17.5%)高于雄猴(5%);D型逆转录病毒抗体阳性猴群的STLV-1抗体阳性率(34.1%)高于野捕猴群(8.8%).结论在我国的野生和饲养恒河猴群中广泛存在STVL-1血清抗体阳性动物,其抗体阳性率与动物的年龄和性别有关.

Abstract:Objective To investigate the role of apoptosis in the pathogenesis of simian acquired immunodeficiency syndrome (SAIDS), we cloned the cDNA fragment of Fas gene in Rhesus monkey. Methods We extracted total RNA from inguinal lymph nodes and designed the upper and low primers according to the Fas gene of human. The fragment was obtained by RT-PCR, and then cloned into PGEM-T Easy vector Finally, the positive clone was sequenced. Results We first cloned the Fas gene of Rhesus monkey, the encoding sequence is 1 005 bp.The sequence was submitted to GenBank, accession number is AY007572. Conclusion The length of encoding sequence in Rhesus monkey Fas gene is different from the homologous genes of human and cynomolgus monkey which have already been submitted to GenBank.

Abstract: