Abstract:Objective To study the different usage of BALB/c and C 57 BL/6,the two strains of laboratory mouse with different genetic backgrounds,in relevant experiments Methods Authors chose antisense oligo-nucleotides of two genes expressed in central nervous system by random sequencing and bioinformatics analysis, injected them into the lateral ventricles of mouse brain using Hamilton micro-syringe respectively There are also two control groups of saline and scramble Each group has ten mice And then several behavioral tests were carried out They are Feeding for metabolic ability,Open Field Behavior for locomotion activity,Tail Flick Latency(TFL)for limen of pain,Step-down Test for memory Results It was found that after the injection of No 1 antisense both two strains of mice showed damaged memory and there were obvious differences between experimental groups and control groups It is said that the function of No 1 gene is correlative with memory After the No 2 antisense was injected,BALB/c strain experimental group showed obvious decrease in numbers of step-across and standing-up in open field behavior test compared with control groups But in C 57 BL/6 mice,there weren't obvious differences between experimental group and control groups And there were also differences between the control groups of the two strains Conclusion The results show that using laboratory mouse strains with different genetic backgrounds can set up a further monitoring system for the screening of novel gene funtion Simultaneously the results have certain significance in finding which kinds of strains are suitable for which kinds of experiments

Abstract:目的　探索BALB/c和C

Abstract:目的　阐明β-葡糖醛酸酶(β-G)在正常恒河猴组织中的分布。方法　对实验恒河猴主要组织的冰冻切片采用后偶合技术进行了染色和显微观察。结果　肾上腺皮质束状带和网状带细胞，肝脏、睾丸、卵巢、胃肠道的实质细胞、枯否细胞、软骨细胞、卵巢间质腺细胞等富含β-G而深染蓝色。肾上腺皮质球状带细胞、血细胞、脑、肌肉、胆囊和胰腺的实质细胞等酶含量少而染色较弱。软骨基质，小脑皮质分子层和颗粒层等部位酶呈阴性。结论　动物的种系和品种不同，器官组织内β-G的含量也不相同。

Abstract:目的　对西双版纳小耳猪的群体遗传结构进行RFLP分析。方法　采用ECL核酸直接标记和检测系统，以HRP标记ɑ-珠蛋白-3’HVR多基因座小卫星探针，对西双版纳小耳猪的Hinf Ⅰ或HaeⅢ酶切片段进行Southern杂交，以化学发光法进行检测，获得了清晰可辨的、具有高度多态性的DNA指纹图谱。结果　2　kb以上的谱带数在6～15条之间，HinfⅠ酶切产生的图带数低于HaeⅢ。JB亚系内805、807家系和JS亚系内111、121、121、151家系不同个体间的相似系数分别为0.94±0.09、0.85±0.08、0.84±1.7、0.78±1.6、0.83±0.42、0.87±0.07，显著高于各家系间的相似系数，JB亚系内的805家系和JS亚系内的151家系不同个体间相似系数较大，分别为0.94和0.87。结论　西双版纳小耳猪近交程度较高，个体间差异较小，均一性较强。

Abstract:目的　研制一种对嗜肺巴斯德杆菌表现出强选择作用的选择性培养基，用于该菌的常规检测。方法　药敏试验及抗生素最小抑菌浓度测定。结果　研制了嗜肺巴氏杆菌选择性培养基(PPSM培养基)及嗜肺巴斯德杆菌增菌液(PP肉汤)。嗜肺巴斯德杆菌在PPSM培养基上，37℃48h培养，形成1mm左右，凸起、湿润、灰黑色并有金属光泽的特殊菌落；对表皮葡萄球菌和大肠埃氏菌的抑制率为100%，对变形杆菌的抑制率为76%，并能抑制其迁徙生长；通过PP肉汤增菌培养，PPSM培养基使SPF小鼠粪便中嗜肺巴斯德杆菌检出率从0增至67.2%；用小鼠咽拭子接种该培养基，其初代培养物几乎为纯培养物。结论　该培养基对嗜肺巴氏杆菌具有较强的选择作用，使用该培养基对嗜肺巴氏杆菌进行检测可以简化检测程序、防止漏检、在不处死动物的情况下对嗜肺巴斯德杆菌进行常规监测。

Abstract:Objective To compare and analyze genetic characteristics of field voles(Microtus fortis)in DNA level,and to distinguish the field voles by RAPD markers Methods Six pieces of 10bp single random primers were selected and used to amplify genomic DNA of Microtus fortis from two regions in China Results 20 fragments of genomic DNA were shared by the field voles from two regions Two pieces of primers could be used as characteristic RAPD markers The genetic background of individuals from two regions was not homogeneous at RAPD markers Conclusion RAPD could be used to distinguish the species(or subspecies) of Microtus fortis from the two regions in DNA level

Abstract:Objective To establish a new model of whole cerebral ischemia/reperfusion in rats on basis of anatomic and hemodynamic principle Methods Whole cerebral ischemia in rats was induced by clamping the bilateral common carotid arteries and drawing some blood from right common carotid artery continuously through right external carotid artery Meanwhile the drawn blood was retransfused into left femoral vein Reperfusion began after stopping drawing blood and removing the artery clamps Electroencephalograph(EEG),light microscope(LM) and electron microscope(EM) were used to assess the result of ischemia Results Compared with control group,the whole cerebral in the experimental group showed obvious ischemia changes in EEG,LM and EM Conclusion This novel model has several advantages such as the reliable whole cerebral ischemia,sufficient reperfusion,high rate of success,fit for injecting reagent through carotid artery,and is useful to the study of whole cerebral ischemia/reperfusion

Abstract:Objective To collect endometrium with complete structure and normal function Methods Three means of cutting,scraping and squeezing were comparatively studied Results The endometrium by squeezing was in the shape of cylinder with integrated tissue structure including endometrial epithelium,gland and capillary Moreover the endometrium situated in the mesometrium was incomplete Conclusion Squeezing the cornua uteri is the practicable way to collect the mouse endometrium

Abstract:目的　研究青光眼对视网膜脉络膜血液循环的影响。方法　选24月龄、体重3.5～4kg的先天性青光眼大耳白兔5只(7只眼)，选10只同龄大耳白兔作为对照组。另选10只2月龄、体重2kg大耳白兔前房内灌注生理盐水制成急性高眼压模型。对三组兔进行眼底照像、闪光视诱发电位(FVEP)检查，观察视网膜脉络膜血管形态和FVEP的变化。对人工急性高眼压组还进行了闪光视网膜电流图(FERG)检查。结果　先天性青光眼组与同龄对照组相比视网膜脉络膜末梢血管网明显减少；人工急性高眼压组眼压升高后首先使视网膜脉络膜末梢血管网灌流不足，随着眼压的继续升高脉络膜大血管变细，末梢血管网灌流不足加重，眼压极度升高时脉络膜大血管血流中断。同龄正常对照组的FVEP的主波P100潜伏期是(83±9)ms，先天性青光眼组则为(112±14)ms，差异有非常显著意义(P＜0.01)；人工急性高眼压组高眼压前为(69±5)ms，眼压60～80mm　Hg时延长为(81±7)ms，眼压在100～130mmHg时FVEP波形低平，近似直线；眼压恢复正常后2hFVEP的P100潜伏期为(82±8)ms。人工急性高眼压前后FERG变化显著。结论　青光眼可以影响视网膜脉络膜血液循环；可使FVEP、FERG发生变化。

Abstract:目的　探寻室内东方田鼠的繁殖与生长发育规律。方法　以F4代鼠及其仔代(F5)为对象，分别观察其繁殖与生长发育情况。结果　室内东方田鼠一年四季均具有繁殖能力，春(3～4月)秋(10～11月)两季为繁殖高峰期；雌雄单一配对比多性比配对的母鼠繁殖率明显提高；母鼠怀孕期20～21d，窝产仔数3～11只，平均(4.8±1.5)只，初生重2.5～4.4g；幼鼠3　d龄耳壳全部竖立，6～8d龄被毛长全，7～11d龄开眼，10～11d牙齿长齐；15d龄左右具有采食能力，20d龄可完全断奶；60～70d龄可见个别雌鼠阴门开孔，75～90d龄可见多数雌鼠阴门开孔和雄鼠睾丸明显下位；3月龄后体重、身长增长不显著。结论　开放式(普通级)饲养环境下，室内东方田鼠的繁殖季节、窝产仔数及初生重与野生东方田鼠基本相似；种群密度、性比对雌鼠的繁殖率有明显影响；2～3月龄为性成熟期，3～4月龄为体成熟和初配时期。但成熟时间存在个体差异，同时受饲料营养和环境因素的影响。

Abstract:目的　建立人肝癌细胞系HCC-9724(简称H)淋巴结转移模型，研究肿瘤转移机理。方法　采用裸鼠肝脏原位移植法，接种肿瘤细胞，取其淋巴结转移灶反复肝内接种，连续传三代后，观察其转移特性，采用SABC法测定淋巴结中nm23和Ⅳ型胶原酶表达。结果　裸鼠原位接种50d，肝内长出约1.7cm×6.0cm大小的肿瘤，呈分叶状，质地较软，周围血供丰富，瘤组织与邻近脏器粘连，有明显的浸润和转移，经裸鼠三次筛选后肿瘤潜伏期短(15d)，瘤体大，形成广泛的肠系膜淋巴结转移，淋巴结中Ⅳ型胶原酶表达呈强阳性；而nm23呈弱阳性。结论　采用裸鼠肝原位移植法，反复筛选，获得了人肝癌淋巴结高转移模型。