Abstract:目的获得东方田鼠的特异DNA序列.方法Aβ基因使用PCR,基因克隆,斑点杂交,DNA序列分析,生物信息学技术.结果根据小鼠MHCⅡ外显子2及其两侧序列,合成引物并扩增东方田鼠基因组DNA,将PCR产物回收、测序后,分别设计内引物扩增东方田鼠基因组DNA,其中一对引物可得到特异性扩增带,将得到的DNA片段插入PGEM-Teasy载体,进行序列分析.用这对引物扩增人、昆明小鼠、BALB/c小鼠及C57BL/6J小鼠基因组DNA,均无扩增产物.以东方田鼠特异性扩增产物为探针进行斑点杂交,除东方田鼠基因组DNA外,其他几种动物基因组DNA均为阴性结果.进一步对该DNA片段进行了BLAST同源性搜索和外显子预测,在Genbank中没有发现高度同源序列,并且找到一个可能的外显子,该外显子由69个氨基酸组成.结论获得的DNA片段为东方田鼠的特异片段,这将为从分子水平深入研究东方田鼠的遗传背景、生物进化规律以及东方田鼠抗日本血吸虫的机理奠定基础.

Abstract:目的观察IRM-2小鼠对电离辐射的耐受性.方法分析测定了IRM-2小鼠对137Csγ射线的LD50及经4.0Gy137Csγ射线照射后不同时间外周血白细胞、骨髓有核细胞总数、骨髓细胞DNA含量和脾结节的变化,并与亲代小鼠ICR和615进行了比较.结果用不同剂量的137Csγ射线照射后,IRM-2小鼠对γ射线的LD50比ICR和615小鼠分别高1.73～1.57Gy和1.44Gy;外周血白细胞数和骨髓有核细胞总数、骨髓细胞DNA含量下降的幅度小且恢复得快;CFU-S的增加也较ICR和615小鼠明显.结论IRM-2小鼠比一般的纯系和杂交品系小鼠具有更强的辐射抗性.

Abstract:目的制备乳腺生物反应器所必需的乳腺特异性表达的调控序列,并验证其指导外源基因表达的能力.方法用PCR法从奶牛染色体上分5段扩增出了全长8.2Kb的牛β-乳球蛋白基因,包括1.8Kb的5′侧翼区、1.7Kb的3′侧翼区及4.7Kb的gDNA区.扩增出的各片段克隆到T-载体上,酶切鉴定及序列分析均证实了所扩增片段的正确性.将五个片段与荧光素酶cDNA拼接成荧光素酶瞬时表达载体并在小鼠乳腺中瞬时表达.结果注射荧光素酶瞬时表达载体的小鼠乳汁中明显测出了荧光素酶活性.结论所克隆的牛β-乳球蛋白基因表达调控序列能够指导外源基因在小鼠乳腺中表达.

Abstract:Objective\ To research genetic type of hybrid field voles from different regions.Methods\ We used 4 10bp random primers to amplify DNA of hybrid field voles from Ningxia and Dongtinghu in China and analyzed the homology of parents and their offsprings.Results\ There were several fragments of genomic DNA shared by all field voles but specific fragments were seen in the parental generation of the two different regions. 4 primers could be used as characteristic RAPD markers to distinguish the subspecies.Parental RAPD markers were found in the offsprings.The homology of field voles of same litter were among 70%-96%.Conclusion\ RAPD could be used to distinguish the species or subspecies of field voles.\;

Abstract:Objective\ To analyze the genetic variations of 3 lines of SPF chickens and to direct the breeding of SPF chickens.Methods\ 30 blood samples were collected from lines F,M,B and the contrast pure line H each.Mixed in equal quantity into 3 pool samples respectively,DNA was extracted,random primers were screened and the response system was optimized,then the templet DNA was amplified.The average similarity coefficient within each line was calculated and the dendrogram was constructed based on inter-line genetic distance.Results\ There were clear and steady amplified bands using 13 of 60 random primers;the average similarity coefficient within 4 lines(B,F,M,H) were 0.903,0.673,0.755 and 0.968 respectively.3 samples of each line were clustered into one group firstly,then line F and line M were clustered into one group,line B and line H were clustered into another group.Conclusion\ The genetic similarity of line B is the highest while line F and line M were much lower.The genetic distance between line F and line M was the shortest,which was in accord with the importing history of the breeds.\;

Abstract:Objective\ To obtain the information of characteristics of reproduction and growth of IRM-2 inbred strain mouse.Methods\ The growth and reproduction performances including litter size, weanling rate,body length, body weight and organ weight were investigated in the IRM-2 inbred strain mouse.Results\ The reproductive capacity of IRM-2 mouse was stronger than the parental strain 615 mouse,for litter size the former was 8.5 and the latter 6.5. The weanling rate observed in IRM-2 strain was 100% and the longest life span was over 39 months. The mean body weight for male and female was 28.9g and 24.6g and the body length 9.6cm and 8.8cm at 10 weeks old,respectively,suggesting that the growing speed of IRM-2 mouse was quicker than that of parental mouse. Organ weight was higher in male than in female,except thymus.Conclusion\ The results showed that IRM-2 is a fine inbred strain mouse.\;

Abstract:Objective\ To establish urogenital carcinoma cell lines and provide a tumor model for further research.Methods\ The tumor tissues were cut into several fragments sized 1.0mm3 then implanted into one nude mouse subcutenousely.If the tumor tissues in the nude mouse appeared to grow and became very large,it then was passaged in nude mice for another two times.The tumor tissues in the third mouse was cut and cultured in vitro primarily.When the cells were cultured more than 20 passages,it was certified according to the references.Results\ 40 specimens were implanted.6 tumors grew in F 1 nude mouse,and 3 in F 3. The 3 tumor tissues were incised and cultured in vitro.Finally 3 infinite tumor cell lines were established:renal carcinoma cell line RCC-9863;bladder cancer cell line BC-6;prostate cancer cell line PC-98106.They are all cultured for more than 1 years and grow steadly.Also they have the human's morphological structure and inherent characters.Conclusion\ Nude mouse tumor implantation model is considered to be a good method to establish infinite urogenital carcinoma cell lines.\;

Abstract:目的应用人工雌核发育繁殖技术建立红鲫近交系.方法红鲫卵子被经紫外线照射的鲤鱼精子激活后,在0～4℃的温度下进行冷休克处理30min,再在常温下静水孵化;以红体色为遗传标记,在连续2代雌核发育子代中选择二倍体红鲫,放置水泥池中常规饲养;用同样的人工雌核发育繁殖技术进行保种,随机交配繁殖1～2代供应实验;用常规方法测量形态学指标.结果红鲫卵子激活率在91%以上;实验组2中摄食期仔鱼成活率为15.20%,80%的成鱼为二倍体红鲫,其中有较高比例的遗传上真实的雄性个体.鳞式主要为27(5)/(6)28;鳍式主要为D.i,17～18;A.i,6;V.i,7～8;P.i,15～16;C.24～25;其他外部形态指标与封闭群红鲫近似.结论本实验建立的人工雌核发育繁殖技术,设备要求简单,操作安全可靠,并且省时节资,完全能够用于培育鱼类近交系,本实验结果完全可以满足育种繁殖的要求;所获得的二倍体红鲫,经同工酶电泳等检测,被证实是纯合的,具备鱼类近交系品系特征;出现遗传上真实的雄性个体是正常的.

Abstract:Objective\ To further identify the nature of newly isolated virus,BV147.Methods\ PCR was used for B virus DNA and the PCR products were sequenced.Results\ The partial gene of newly isolated B virus showed 99.54% nucleotide identity over the corresponding region of strain E2490 of B virus in America,while only 89.91% over that of SA8.Conclusion\ The newly isolated culture was demonstrated to be B virus.\;

Abstract:Objective\ To compare biological characteristics of EAC cells from 3 different institutes.Methods\ Cell size was determined with the Cells Analysis System.The curve changes of neoplastic diameters were studied after subcutaneous injection of EAC in BALB/c.The neoplastic weights of the 12th day were measured and the sensitivity of EAC to CTX in BALB/c was compared.Results\ Both size and shape of cells were basicly of similarity.Ectogenesis of Beijing EAC was easier than the other two EAC. Induced tumors of Beijing EAC were the most by subcutaneous inoculation. The tumor-inhibitory rate to CTX treatment was 82.9%(Beijing EAC),71.9%(Wuhan EAC),50%(Shandong EAC) respectively. Conclusion\ The EAC cells from 3 different institutes appeared to be different markedly.\;