Abstract:Objective To establish a mouse model of photochemical induced acute cerebral infarction.Methods\ The orthogonal test with 5 factors and 4 levels was used to study the best experiment conditions of photochemical induced acute cerebral infarction in mice.Results\ The best experiment conditions: exposure time is 5 minutes,exposure rate is 1,dosage of rose bengal is 100?mg/kg,dosage of evans blue is 100?mg/kg,the time put to death is 4 hours.Conclusion\ The verification of the result proves that the experiment conditions of the model are correct.

Abstract:目的建立DNA指纹技术对近交系大鼠遗传监测的方法.方法采用DNA指纹图法对国内已知的7个品系9个近交系大鼠群体进行了分析,并对相同DNA进行了多次DNA指纹图重复实验.结果(1)不同品系之间DNA指纹图差异较大,其平均图带数为16.360±2.178,共有带率为0.061±0.008,相似系数为0.062±0.008,相同DNA指纹图概率为3.691×10-23.(2)同一群体不同个体之间DNA指纹图带的相似系数和共有带率(除SHR(哈)和WKY(哈)小于0.7外),其他均在大于0.9.(3)不同地区同一SHR和同一WKY品系间DNA指纹图也存在不同,其相似系数和共有带率均小于0.6.(4)相同DNA不同次制作的DNA指纹图谱基本一致(P>0.05).结论证实了该方法在近交系大鼠遗传监测中的可行性.

Abstract:目的明确人SR-AI转基因小鼠的一些形态、脏器、血液学和血浆脂质参数,为人SR-AI转基因小鼠模型的应用提供相关依据.方法用游标卡尺和电子称量天平测量人SR-AI转基因小鼠的吻长、眶间距、体长、尾长及12个脏器系数并与C57BL/6J小鼠进行比较分析;用SysmexK-4500型全血自动多参数血液分析仪检测其血液学指标;并用酶法分别测定血浆总胆固醇、甘油三酯和高密度脂蛋白胆固醇含量.结果人SR-AI转基因小鼠外部形态指标和脏器系数与C57BL/6J小鼠比较差异没有显著意义(P>0.05);转基因小鼠的各项血液学指标无明显改变,血脂蛋白成分的改变亦未显示统计学意义(P>0.05),甘油三酯的浓度略有增高(P<0.05).结论人SR-AI转基因小鼠的外部形态指标、脏器系数及各项血液学指标均无明显改变,适合用作动脉粥样硬化模型的研究.

Abstract:Objective To sequence and express tissue type plasminogen activator (tPA) cDNA of rhesus monkey. Method\ The cDNA was obtained by RT PCR from the lymphocyte of rhesus monkey, of which the primers were designed according to human tPA cDNA sequence. The amplified fragment was then cloned into T vector. Results\ The result of nucleotide sequencing showed the coding region of rhesus monkey tPA to be 96% nucleotides and 97.5% amino acids identity with human tPA. In the subsequent experiment, the rhesus monkey tPA cDNA was cloned into eukaryotic expression vector, and tPA activity of 50 U/ml can be detected in the medium at the second day (48hours) after transfection of CHO cells. Conclusion\ The coding region cDNA sequence of rhesus monkey and its expression in mammalian cells are reported. It could be useful for the further study of tPA biological characters.

Abstract:目的用重组蛋白抗原取代淋巴细胞脉络丛脑膜炎病毒(LCMV)抗原用于实验动物感染LCMV检测.方法合成LCMVsRNA第1816～2178位核苷酸序列编码的Np第380～500位氨基酸基因,克隆在Hisx6-GSTpET-28a载体中,表达产物经固定化金属配体亲和层析纯化,建立ELISA检测试剂.结果合成基因表达产物经Ni-NTA-Agarose纯化后纯度达到95%以上.ELISA检测小鼠、地鼠和豚鼠结果与全病毒抗原基本一致,而且特异性较强,操作简便.结论用合成基因表达产物取代LCMV抗原检测病毒抗体,不仅可以消除病毒传播可能,而且特异性强.为实验动物及生物材料质量控制提供安全有效方法.

Abstract:Objective To identify the newly isolated virus for detection of B virus DNA. Methods\ Suspected virus cultures were detected for B virus DNA using PCR method and the PCR products were sequenced. Results\ The partial gene of newly isolated B virus showed 100% nucleotide identity over the corresponding region of E2490 strain of B virus in America. Conclusion \ The newly isolated is B virus.

Abstract:Objective To establish the animal model of Qi dificiency and blood stasis syndrome of cerebral ischemia.Methods\ The animal model of Qi dificiency and blood stasis syndrome was formed by starvation,fatigue,cold wetness,panic,high grease diet in old Wistar rat. The animal model of cerebral ischemia was formed by bilateral carotis communis artery obstruction(CCAO).The general macroscopic and microcosmic indicators were monitored.Results\ The model rats are almost in accordance with the peculiarity of Qi\|dificiency and blood stasis syndrome and the pathologic rule of cerebral ischemia.Conclusion\ The study suggests that this kind of animal model of combination of disease with syndrome could be used in the study of preventing and curing the cerebral ischemia by traditional Chinese medicine.

Abstract:Objective To study the distribution of the lactate dehydrogenase isoenzyme in different tissues of field voles ( Microtus fortis ). Methods The samples were studied by polyacrylamide gel electrophoresis (PAGE) and stained by sodium lactate. Results There was only LDH 5 in plasma of field voles from all three regions and in sera of field voles from Dongting region and from Ningxia Qingtongxia region. There were two kinds of LDH isoenzymes(LDH 1 and LDH 5)in sera of field voles from Dongbei region. Five kinds of LDH isoenzymes were found in kidney and lung of field voles from three regions and in liver of field voles from Dongbei region, but LDH 5 was dominated in liver of the two other field voles(more than 95%). Conclusion\ LDH isoenzymes of field voles from Dongbei region were different to some extent from the two other regions.

Abstract:Objective To examine the difference of mRNA expression in S180 cell strain and its different cloned cells.Methods The probes labeled by biotin were used in situ hybridization to detect the expression of 6 kinds of mRNA that were p16, c fos, c jun, c myc, p21 and p53.Results P16,c fos, c jun and c myc mRNAs were found in Cancer Institute S180 cells, c fos and c jun mRNAs in S1B11 cloned cells, c fos, c myc and c jun mRNAs in S2D9 cloned cells. Conclusion The expression of mRNAs was different in Cancer Institute S180 and 2 different cloned cell strains and c myc probe could distinguish S1B11 from S2D9 cloned cells; expression of mRNAs was associated with carcinogenesis.

Abstract:目的探讨人的重组促黄体生成素(r-hLH)对性成熟前的小鼠卵母细胞体外受精(invitrofertilization,IVF)后的分裂、以及IVF胚的体外培养(invitroculture,IVC)过程中发育状况的影响.方法选用性成熟前的清洁级昆明小鼠,分别用r-hFSH(人的重组促卵泡素20IU),r-hFSH+r-hLH(各20IU)或20IUr-hLH对其进行超排处理,通过对从各处理组小鼠卵巢中手工器械分离出来的卵丘-卵母细胞复合体(cumulusenclosedoocyte,CEO)进行体外受精之后,进一步观察这些CEO经IVF后的受精分裂状况,以及IVC后的发育情况.结果r-hFSH+r-hLH处理组小鼠卵母细胞IVF率(72.2%)极显著高于r-hFSH单独处理组(39.9%)或r-hLH单独处理组(37.2%)的相应指标;r-hFSH+r-hLH处理组和r-hFSH单独处理组的IVF胚,发育至8～16细胞阶段的比例(8～16细胞率)趋于一致(25.15%∶27.18%),但是都极显著高于r-hLH处理组的8～16细胞率(17.24%);r-hFSH处理组IVF胚发育至16～32细胞阶段的总比例(16～32细胞率∶33.01%),极显著高于r-hFSH+r-hLH处理组及r-hLH单独处理组的指标(1.75%和0).结论在IVF条件下,r-hLH对小鼠卵母细胞的受精有明显的促进作用,同时却对相应IVF胚的进一步发育产生明显的抑制作用.

Abstract:目的建立发光细菌综合毒性测定法,对实验动物饲料原料中有毒有害物质进行初筛.方法用发光细菌作指示生物,测定金属离子Pb2+,Zn2+,Cr+,Hg2+和毒性物质苯酚,灭害灵;以Hg2+为毒性对照物,测定10种饲料原料和3种人用食品.结果发光菌对有毒有害物质和金属离子敏感.在所测定的范围内,其发光强度随浓度增加而减弱,即相对抑光率增强,呈负线性相关,其相关系数在0.92以上;以Hg2+为毒性对照物,对10种饲料原料和3种人用食品进行测定,其中脱水蔬菜相对抑光率最大,为39.74%,相当于1.85×10-5%Hg2+的表征毒性,提示该样品可能受到农药和化肥等较严重的污染.结论发光细菌综合毒性测定法,可用于饲料原料中有毒有害物质的初筛,是一种快速、经济、有效的方法.

Abstract:Objective To investigate the role of stress in the tendonization of HHAT. Methods The rabbits were divided into two groups at random. In the experimental group, the HHAT was used to connect the two ends of the cut Achilles tendon. In the control group, HHAT was placed in the dorsal muscle of the rabbit. Pathological changes and mechanic properties of HHAT were observed respectively. Results In the experimental group, with the role of the stress, the fibroblasts were induced to proliferate and excrete collagen fibers and form a collagen sheath around the HHAT. In the meantime, HHAT degradated step by step. At last ,HHAT was replaced by collagen tissue. In the control group,without the role of stress, the maximum load and the tensile strength of HHAT in muscle decreased. Finally, HHAT was absorbed completely. Conclusion HHAT could degradated and be absorbed compeletly. The stress was an important factor in the process of tendonization.

Abstract:Objective To survey the infectious rate and strain of M.pulmonis in the rodent laboratory animal in Lanzhou districton. Methods The M.pulmonis infection of 640 rodents labovatory animals in Lanzhou were surveyed by culture method. The isolates were cloned and identified by morphology, biochemical features and serology. Resultsd and conclusion The infectious rate of M.pulmonis was 23 % in the conventional mice.No infection were fond in rat, guinea pig and hamster. There was no seasonal correlation to M.pulmonis infection in the mice. According to theose experiments, the isolates were Mycoplarma pulmonis, Mycoplasmataceac, Mycoplasmatales.

Abstract:Objective To detect and analyse hematologic,serum biochemical and hemorheologic values in rhesus monkeys in anesthesia and unanesthesia.Methods Blood samples were obstained from 48 healthy rhesus monkeys in unanesthesia,30 blood samples obstained in anesthesia.All the samples were tested with automatic blood cellular analyzer,automatic blood biochemical analyzer and N6A automatic washing viscosimeter.Results In the anesthesia compared with unanesthesia,the values of GGT,AST,CREAT,TBIL,DBIL,P 3 ,TF in serum biochemistry,RBC,Hct,MCV,MPV,RDW,NEUTRO in hematology and plasma viscosity were significantly lower ( P <0.01),the values of ALT,ALP,LDH,HBDH were lower( P <0.05),on the contrary values of UA,Ca 2 ,CK MB,ALB,MCH,MCHC,PLT,LYM and MEDIAN were higher( P <0.01).Conclusion These reference values were affected in rhesus monkeys anesthetized.

Abstract:目的探讨不同剂量的大肠埃希菌内毒素(LPS),实验室的温湿度、动物的固定方法和动物的生理机能状态对兔发热反应的影响.方法实验在能控制温湿度的实验室内进行,静脉注射不同剂量(0.5?ng/kg\,2?ng/kg\,20?ng/kg\,100?ng/kg\,200?ng/kg\,2000?ng/kg)大肠埃希菌内毒素(Lipopolysaccharide,LPS)诱发兔发热反应.结果随LPS注射剂量的增加兔发热反应增强.表现在发热潜伏期逐渐缩短;发热高峰值逐渐增加;发热时程逐渐延长;体温反应指数逐渐加大.另外LPS注射剂量不同,动物发热曲线的峰型亦不相同,小剂量LPS(0.5?ng/kg及2?ng/kg)可诱发单峰型发热反应;用量增加到20?ng/kg以上时,呈双峰型发热反应.静脉注射剂量为100～200?ng/kg时兔发热反应呈典型的双峰热,且发热反应均一,是造模的适宜剂量.实验结果证明实验室的温度为25℃左右动物以颈部固定方法为宜.

Abstract:Objective To detect the expression of iNOS gene in brain tissues from limb ischemia\|reperfused SD rats.Method SD rats were randomly divided into three groups:limb ischemia\|reperfusion group(I\|R), simple ischemia group(I) and normal control group(N).The expression of iNOS mRNA in brain was semiquantitatively detected by using of RT\|PCR.The distribution and the expression of iNOS and peroxynitrite protein in brain were measured by immunohistochemistry.Results iNOS mRNA was not detected in brain from control rats,while it was detected in both I and I\|R groups.After I\|R, the expression of iNOS mRNA gradually increased at 2h and peaked at 6h( P <0.01 vs I group),then decreased,but there was also a little expression at 24h.The morphologic results showed that there were more iNOS positive cells and more dispersal NT positive neurons in I\|R group than I group.Conclusion The expression of iNOS mRNA in brain significantly increased after limb ischemia\|reperfusion in a time\|dependent manner.