Abstract:ObjectiveTo acquire cDNA sequence of tree shrew CXCR4 and to understand whether tree shrew CXCR4 (tsCXCR4) supports the HIV_1 binding. MethodsThe corresponding designed primers, RT_PCR, gene cloning, DNA sequencing analysis were used. ResultsThe clone of full_length tsCXCR4 cDNA is 1059bp. The homology of tsCXCR4 with that of human CXCR4 (hCXCR4) is 92 8% at nucleotide level and 96 9% at deduced amino acid level. Tyr residues are at positions 7 and 12, and Glu residues are at positions 14,15 and 32 in th e NH 2 _terminal extracellular domain of the tsCXCR4. In addition, Arg residues at positions 183 and 188, and Asp residues at positions 193, 262 and 97 are found in the extracellular loops and transmembrane domains of the tsCXCR4, the above residues are the same as hCXCR4, and are essential for the function of hCXCR4. ConclusionThese results suggest that tsCXCR4 may contribute to the entry of X4 and R5X4 strains of HIV_1.

Abstract:ObjectiveTo study the isolation, culture and characterization of neural stem cells from newborn rat spinal cords. Methods\ Cell culture technique and indirect immunoflurenscence cytochemistry were used. Results\ The cells were proliferated vitally. Neurospheres achieved by single cell cloning were positive with incoporation of BrdU and Nestin immunoflurescence staining. The cells had survived and grown for 8 months in vitro. The neurospheres could be induced to differentiate into neurons and astrocytes by 1% calf serum. Conclusions\ Neural stem cells from newborn rat spinal cords were cultured successfully.

Abstract:目的　为子宫内膜异位症 (EM)的临床研究提供实验动物模型。方法　实验组 :取EM和正常育龄妇女分泌晚期子宫内膜 ,剪碎 ,随机置入裸鼠盆腹腔 ,实验Ⅰ组切除卵巢 ,实验Ⅱ组不切除卵巢 ;对照组 :同法置入人大网膜。术后给雌激素支持。各组裸鼠分别于 5、10、15、2 0、2 5、3 0d脱颈椎处死 ,观察异位病灶生长情况。免疫组化SP法检测异位病灶雌、孕激素受体表达。结果　子宫内膜种植 5d即见异位病灶牢固粘附 ,主要发生在腹壁(56% )、膀胱旁脂肪 (2 1% )等部位 ,可见腺体细胞及散在间质细胞 ,时间越长 ,病灶与裸鼠组织融合越明显 ,盆腹腔粘连越重。用EM患者和正常育龄妇女内膜移植的异位病灶无差别 ,实验Ⅰ组和实验Ⅱ组的裸鼠所形成的异位病灶亦无差别 ,它们的雌、孕激素受体表达未显示差别 ,多数呈弱表达。结论　裸鼠做为EM早期动物模型科学、简便、可行。

Abstract:目的　构建L3 2_pGEX_5x_2重组质粒 ,诱导表达重组钩端螺旋体外膜脂蛋白LipL3 2。方法　PCR获取编码LipL3 2的基因片段 ,构建重组克隆载体和表达载体 ,转化受体菌 ,诱导表达重组LipL3 2蛋白。将重组LipL3 2蛋白和钩体抗血清进行Western_blot。结果　扩增出约 750bp的LipL3 2成熟蛋白基因 ,LipL3 2基因插入pGEX_5x_2表达载体 ,表达产物谷胱甘肽S_转移酶 (GST ,2 6× 10 3)与LipL3 2蛋白的融合蛋白的相对分子质量约为 53× 10 3 ,与预期大小一致。Western_blot显示重组LipL3 2蛋白能与钩体抗血清特异结合。结论　LipL3 2蛋白能在大肠埃希菌中表达 ,重组LipL3 2蛋白具有免疫反应性

Abstract:Objective To study the activation extent of reactive astrocytes in br ain of PDAPP transgenic mice. Method\ The activity degree of reactiv e astrocytes in brains of PDAPP transgenic mice was compared with that of C 5 7 /BL non-transgenic mice through detecting the expression of (glial fibrillar y acidic protein(GFAP) on reactive astrocytes using immunohistochemistry. Results\ The levels of GFAP expression on reactive astrocytes in brains fr om PDAPP tra nsgenic mice was significantly higher than that of C 57 /BL non-transgenic mice. Conclusion\ There could be significant neuroinflammation in brain of PDAPP transgenic mice.

Abstract:ObjectiveTo establish a method of the endomet r iosis in SD rats during non-estrus and study its histological structure and hum oral immunity.Methods\ Endometriosis was induced in 40 female SD rat s during non-estrus and estrus respectively by surgery of self-endometrium tra nsplant.Before and after the operation,its blood was collected for observing IgG ,IgA,IgM,C 3,C 4.The endometrium of the uninjured side and the ectopic endomet rium were taken for histological observation.Results\ There was no s i gnificant difference between the successful rates,95% of non-estrus contrast to 94 8% of estrus by this method.The function of the ectopic endometrium epithel ial cells was activated,with similar altered cycle or physiological characterist ic as their normal endometrium.After operation,IgG and C3 were increased(P< 0 01,P<0 05);IgM reduced(P<0 01) and with no significant change f or IgA, C4.Conclusion\ The endometriosis model of SD rats can be successfully mad e both during non-estrus and estrus by self-endometrium transplant.

Abstract:Objective To create a new animal model for atherosclerostic resten osis induced after percutaneous transluminal angioplasty (PTA).Methods\ 18 Japanese white rabbits were selected including 6 in the normal group. The both co mmon carotid arteries were stimulated by electric current generated by a thrombo sis inducer. The rabbits were fed by high-fat diet for 6 weeks after the operat i on. The models were sacrificed and the arteries were obtained at 3 days,1 wee k,3 weeks and 6 weeks respectively after the operation. Then the pathological sl ides were prepared and stained for optical microscopic observation. Result s\ Within 1 week after the operation there was thrombi-g e neration in the models' arteries; endothelial cell hyperplasia and pa rtly internal plastic membrane broken in tunica intima; minor SMC(smooth mus cle cell) edematous or disappeared in tunica media; neutrophil infiltrated in tunica adventitia. The status beyond 3 weeks after the operation was obviously hyperplastic in tunica i n tima and lipid-deposited under it; partly SMC-decreased,both fibre and ground-substance hyperplastic in tunica media, especially in the stimulated par ts; slightly stimulated parts thickened in tunica adventitia. All the 6 animals' a rt eries 6 weeks after the operation were stenosed. Conclusion\ The i ntegra tion of stimulating common carotid arteries by electric current and feeding by h igh-fat diet can induce the animal model of atherosclerostic stenosis successfu lly. The model's pathologic change is similar to human atherosclerosis. Additi onally, the arteries' blind ends generated by the method of PTA can be avoided.

Abstract:ObjectiveTo analyze the genetic purity of Chinese hamsters E Genealogy inbred strain. Method31 RAPD primers were selected and used to amplify 12 sam ples of E genealogy of Chinese hamster. The amplified fragment bands were analy zed to calculate the similarity index.Result In this study ,the similarity index of all samples ranged from 94 3 1% to 99 78%, averaging at 97 49%. ConclusionChinese hamster of Shanyi colony E genealogy has reached a high degree of genetic purity.

Abstract:ObjectiveTo analyze the maturation rate and fertilization ratio of goat oocytes cultured in different mediums for different time or spermatozoa were incubated in two dif ferent mediums.Mothods\ The COCs were cultured in mediums supplemen ted with FCS, EGS or different concentration of follicular fluid and were cultur ed in the same culture medium for 16hrs,20hrs,24hrs or 27hrs,spermatozoa were ca pacitated in medium supplemented with heparin or IA 23187.The maturation rate a nd the fertilization ratio were statistically compared. Resullts\ Oocytes cultured in medium supplemented with FCS or EGS for 27hrs had highe r matured rate and fertilization ratio.Sperm capacitated in medium supplemented with IA 23187 had higer capacitation rate and fertilization ratio.Co nclusion\ COCs cultured in medium supplemented with FCS or EGS for 27hrs and sperm capacitated in medium supplemented with IA 23187 is an efficient IVF s ystem.

Abstract:目的　研究在山羊发情后期三种不同超排方法的超排效果。方法　实验以 2 0只本地山羊为实验材料 ,以氯前列烯醇二次注射法进行同期发情 ,研究了于发情后期 (发情结束后第 2天 )进行 3种不同的超排方法 (对照组 :3 0 0IUFSH 6次减量法 ,F -pvp组 :3 0 0IUFSH溶于 3 0 %PVP一次注射 ,F -pmsg组 :先注射 2 0 0IUFSH ,2 4h后结合 3 3 0IUPMSG一次注射法 )的超排效果。结果　二次注射氯前列烯醇在 60h内同期率为 80 % (16 2 0 ) ,3种超排方法平均获黄体数分别为 9 75± 4 65,11 75± 8 3 4 ,11± 9 13 ;平均获可用胚数分别为 7± 2 94,8± 5 48,7 5± 5 80 ;胚胎回收率分别为 62 16% ,68 0 8% ,61 3 6%。t检验证明实验组 (F pvp组和F pmsg组 )平均获黄体和平均可用胚与对照组差异有显著性 (P <0 0 5) ,而胚胎回收率 (卡方检验 )差异无显著性 (P >0 0 5)。结论　山羊发情周期的发情后期进行超排能取得很好的卵巢反应 ,而且FSH一次注射法 (溶于 3 0 %PVP或 2 4h后结合少量PMSG)与多次减量法的超排效果一致 ,这表明 ,在山羊发情后期使用FSH一次注射的超排方法有可能作为一种简化的体内生产胚胎的方法加以进一步开发和应用

Abstract:ObjectiveTo observe the influence of different blood glucose levels and the influence of glucose before and after hypoxia-ischemia(HI) on the body weight and brain w eight of HI neonatal rats. MethodsNeonatal rat models with HI and hyperglycemia or hypoglycemia were prepared. Then the body weight was sca led before decapitation at the time of 2 h, 24 h, 48 h, 72 h and 7 d after HI an d brain weight of rat models was scaled immediately after decapitation. The sy nthesis of cerebral GLUT1 and GLUT3 in neonatal rats was explored with immunohis t ochemical method at 24h after HI. Results Body weight and b rain weight of the group with hypoglycemia before and after HI were significant ly lo wer than those of other groups at the time of 24 h after HI; brain weight of hyp o xia-ischemia neonatal rat models with hyperglycemia before HI was higher than t h at of HI group and group with hypoglycemia before HI at the time of 7d after HI. At time point of 24h the synthesis of GLUT1 in group with hyperglycemia before HI was significantly higher than that of other groups, while the synthesis of GL UT3 in group with hypoglycemia before HI was significantly lower than that of ot her groups.ConclusionHypoglycemia before HI is disadvantageous for the increase of body weight and brain weight, while hyperglycemia before HI is to some extent protective by alleviating the decrease of body weight and bra in weight by HI.

Abstract:ObjectiveTo investigate SARS virus in animals . MethodsThe secretions of respiratory t ract and swallow swab were examined by using nested RT-PCR,and the sera were det ected by ELISA. Result and conclusion84 swab samples w ere collected from monkeys, dogs, paguma larvata, etc. No SARS coronavirus s equen ces were amplified from these samples. Murine coronavirus antibody of 52 sera i s positive from 373 mice sera and 198 rat sera detected by ELISA. Investigation of source of SARS is very important for the managemetn of SARS.

Abstract:Collagen-induced arthritis (CIA) is a widely-used experimental animal model o f human rheumatoid arthritis(RA) which shares certain features with RA, such as in pathogenesis and pathology. Recent research demonstrates that some activated T cells are critical to the development of CIA, especially during the initial ph ase. All suggests that CIA is kind of dominant T cell-mediated autoimmune disea se.