Abstract:目的　以hIL 1 β为炎症抗原诱导兔膝关节炎 ,观察评价其关节炎和滑膜炎的临床及病理特点。 方法　对通过逆转录病毒载体 (MFG)导入hIL 1 β基因的HIG 82细胞进行体外培养和筛选 ,之后注入 2 4只新西兰白兔的膝关节腔中 ,以注入异体兔滑膜细胞的 8只兔作对照。观察兔全身情况、膝关节局部及滑液的变化和滑膜炎的病理特点 ;并用RT PCR和ELISA法检测hIL 1 β的表达。 结果　转染的兔滑膜细胞系表达大量hIL 1 βmRNA ,分泌hIL 1 β浓度每毫升达 2～ 1 5ng 1 0 5细胞。关节炎滑膜组织有hIL 1 βmRNA的表达 ,关节腔灌洗液中hIL 1 β的浓度介于 2 0～ 6 0pg ml之间 ,血清中未检测到hIL 1 β。关节炎兔的血沉 (ESR)显著增快 ,是对照组的 1 0～ 1 5倍 ,并有发热、体重下降、厌食等全身表现 ;膝关节肿胀明显 ,直径较对照组增加 2 0 %～ 30 % ,滑液中白细胞数显著增加 ,达1 0 1 0 ～ 1 0 1 2 L ;关节炎的严重程度与注入关节腔的MFG hIL 1 β转染兔滑膜细胞数目成正相关。滑膜组织呈结节样增生 ,病理组织学分析显示 :滑膜细胞明显增生 ,可见栅栏样排列的滑膜细胞 ;滑膜基质增生显著 ,有大量中性粒细胞和淋巴细胞浸润 ,其间可观察到淋巴细胞聚集 ,并有纤维素样坏死和血管新生现象。滑膜炎在注入hIL 1 β 细胞后的

Abstract:Objective To breed and establish a new wild derived inbred strain of mice trapped in China, and select its important biological characteristics for enriching present gene bank of laboratory animals. Methods Wild mice were inbred strictly according to the international standards, that is, brother sister cross breeding by the same litters. Results Through 12 years of inbreeding, TW (Tianjin Wild) mice were inbred to F22 generation. And mouse strains from other 15 trapping localities were all interrupted or stopped by genetic reasons. Conclusion This study may enrich international gene bank of laboratory mouse and lay a foundation for establishing the laboratory mouse model of anti-lung tumors.

Abstract:Objective To establish clean homozygote Neo r trangenic mouse strain.Methods Ten clean offsprings of Neo r transgenic mice free of 10 microorganisms infection were obtained through bio cleaning of embryo transfer. Among them five mice were identified as Neo r positive by PCR and Southern blot. The positive mice were bred by brother sister breeding methods to produce homozygotes. Results Four homozygote Neo r transgenic mice were selected through PCR, Southern blot and breeding experiment to form Neo r transgenic mouse strain. The mouse embryonic fibroblast (MEF) prepared from fetuses of female mouse mated with male Neo r transgenic mouse showed resistance against G418 as high as 1 mg/ml in the culture medium. This Neo r MEF can be used as feed cell layer when ES cell gene targeting is conducted. Conclusion The above results show that we obtained clean animals through embryo transfer, a genetical homozygote Neo r transgenic mouse strain.

Abstract:Objective To rescue homozygous (LO) lipoprotein lipase (LPL) knockout mice that should have died in 48 hours after birth by somatic gene transfer using a beneficial mutant of LPL gene (LPL447). Method A recombinant adenoviral vector expressing LPL447 was delivered to the neonatal mice by intramuscular route. The phenotypes of rescued mice were analyzed. Results 75% of LO mice survived the entire suckling period and lived for up to normal adulthood, which is greatly higher than the survival rate achieved by other groups abroad using wild type LPL gene. Adult LO mice exhibited extremely high triglyceride levels.Conclusion Adenovirus mediated LPL447 gene transfer during neonatal stage can rescue the LO mice successfully. Adult LO mice can be used as a severe hyperlipemia animal model for further investigation.

Abstract:目的　测试猕猴———红面猴 (M .arctoides)和恒河猴 (M .mulatta)对花生香、香蕉香和体味的嗅觉阈限。方法　本研究采用以Laska设计的用于测试灵长类动物嗅觉的实验仪器为模板自制的实验仪器 ,通过嗅觉引导觅食的实验任务来测查猕猴的嗅觉阈限。结果　行为训练过程中 ,两只被试在 32 0个试次内均掌握了仪器操作规则 ;嗅觉测试中 ,红面猴 (盼盼 )对花生香的嗅觉阈限是 1∶4 0 0 0 ,恒河猴 (美子 )是 1∶6 0 0 0 ;对香蕉香 ,盼盼的觉察阈限为 1∶30 0 0 ,美子为 1∶1 0 0 0 0 0 ;美子对体味的觉察阈限接近 1∶1 0 0 0 0 0 0 ,而盼盼未完成此项实验。结论　猕猴的嗅觉能力并不像想象中的那样差 ,同时进一步验证了Laska的实验仪器和实验范式的准确性和适用性

Abstract:目的　观察ATP所引起的蟾蜍椎旁交感神经节细胞电位变化和有关因素的影响 ,以探讨ATP在神经节内的作用及其机制。方法　采用细胞外微电极技术记录离体灌流神经节电位。结果　外源性ATP(2 0 0 μmol L)可引起去极化 (n =1 1 0 ) ,超极化 (n =33) ,以及去极后伴随一超极过程的双相反应 (n =1 2 )。P2 嘌呤受体拮抗剂ci bacronblue 3GA(30 0 μmol/L)和奎尼丁 (30 0 μmol/L) ,均可抑制ATP所致去极反应 ,其幅值分别减小 (2 4 .2± 1 7.5 ) %(n =1 8,P <0 .0 1 )和 (32 .5± 1 1 .5 ) % (n =2 1 ,P <0 .0 1 )。P1 嘌呤受体拮抗剂氨茶碱 (2 0 0 μmol L) ,可抑制ATP所致超极反应 ,幅值减小 (6 5 .0± 2 2 .9) % (n =9,P <0 .0 1 )。在无钠溶液中 ,ATP的去极幅值变化为 (1 3.6± 1 7.5 ) % (n =1 0 ,P >0 .0 5 ) ,无统计学意义。在无钙溶液中或同时加用EDTA(1mol L)以去除溶液的Ca2 ,可使ATP所致去极幅值减小 (2 3.6± 1 8.3) % (n =1 5 ,P <0 .0 1 )。电压依赖性钾通道阻断剂 4—氨基吡啶 (3mmol L)和ATP敏感性钾通道阻断剂格列苯脲 (1 0 0mol L) ,均可可抑制ATP所致超极反应 ,其幅值分别减小 (6 4 .5± 2 1 .9) % (n =1 1 ,P <0 .0 1 )和(6 6 .4± 2 2 .4 ) % (n =6 ,P <0 .0 1 )。前列腺素合成酶抑制剂吲

Abstract:目的　研究多氯联苯 (PCBs)暴露对剑尾鱼 (Xiphophorushelleri)超氧化物歧化酶 (SOD)活性的影响 ,探讨剑尾鱼器官组织内SOD活性变化作为环境风险评价 (ERA)的有效生物学标记的可行性。方法　测定了PCBs对剑尾鱼 30d的半致死浓度 (LC50 ) ;使用浸浴法以 0、2、5 0 μg L三个PCBs浓度为剑尾鱼染毒 ,定量测定了 72h内肝、鳃及卵巢组织中的SOD的活性变化。结果　PCBs对剑尾鱼的 30dLC50 为 1 0 5 80 μg L ;PCBs对剑尾鱼肝脏和卵巢的SOD活性有明显 (P <0 0 1 )的影响。在最初染毒的 1 2h内 ,SOD活性略有上升 ,但随暴露时间延长 ,浓度增加 ,肝和卵巢的SOD活性均呈下降趋势。此外 ,结果还显示肝组织的SOD活性较高于卵巢的SOD活性 ,表明不同器官组织的SOD活性对PCBs胁迫的敏感性存在一定的差异。实验中鳃组织SOD活性在PCBs暴露后其变化不明显 (P >0 0 5 )。结论　表明剑尾鱼肝细胞SOD活性可作为环境风险评价 (ERA)的有效生物学标记

Abstract:Objective The relationship between the genetics polymorphism and phenotype of dog coat color was analyzed in order to give advice for the dog breeding. Methods The loci of R306ter in the canine MC1R gene were detected through the technology of PCR SSCP, and the relationships between the polymorphism of R306ter and coat color trait were analyzed. Furthermore, fragments with the polymorphism at R306ter were sequenced. Results\ By the method of PCR SSCP, the R306ter polymorphism was found with two alleles C and D and three genotypes CC, CD and DD. According to the results of sequencing of R306Ter, one terminal change from CGA to TGA at the position 306 was found. Conclusion\ According to the statistical analysis, no significant associations between the polymorphism of MC1R gene and the coat color were found and these results may be due to the differences of genetic background. For the variations of R306ter loci in MC1R gene, the polymorphism of PCR SSCP in the cross breed canine was detected.

Abstract:目的　探讨合成化合物No 1 886调节糖、脂代谢的作用机制及对贵州小香猪胰岛结构和功能的影响。方法　 3- 4月龄贵州小香猪 1 5只 ,随机选取 5只饲以基础饲料为正常对照组 ;5只饲以高脂高糖饲料为糖尿病对照组 ;5只前 3个月饲以高脂高糖饲料 ,后 5个月饲以高脂高糖饲料加 1 0 %No 1 886为糖尿病治疗组。分别在 0、2、3、4、5、6、8个月末时从禁食过夜的猪眶静脉窦取血样 ,测定血浆葡萄糖、甘油三酯和血清胰岛素浓度。第 8个月末 ,处死动物 ,每只动物取胰腺 1小块 ,1 0 %中性甲醛液固定 ,常规石蜡包埋切片 ,HE染色。另一部分胰腺切片用猪胰岛素抗体作免疫组化染色 ,显微观察照相 ,并用图像分析软件IMAGEJ测量胰岛 β细胞在胰岛中所占的面积。另取 1小块胰腺 ,4℃ ,2 5 %戊二醛固定 ,常规透射电镜样品制备 ,透射电镜下观察并照相。结果　糖尿病对照组和糖尿病治疗组 (加药前 )出现高血糖、高甘油三酯 ,胰岛素水平亦升高 ,正常对照组血糖、甘油三酯、胰岛素未见明显升高。两组比较差异具有显著性意义 (P <0 0 5 ) ;与糖尿病对照组比较 ,糖尿病治疗组加药后血糖、甘油三酯、胰岛素水平降低。从胰腺显微和超微结构观察及 β细胞免疫染色结果看 ,高糖高脂饲料对贵州小香猪胰腺组织结构、β细胞结构和功能都

Abstract:Objective Thirty three indexes of hematological and biochemical parameters in eighty mongolian gerbils were mensurated and compared between different sexes and regions. Methods The blood of laboratory animals was taken from eyeball after fasting of 12 hours for measuring hematological and biochemical parameters. Results Most hematological and biochemical parameters were influenced by age difference,and there were no significant differences by sex except GLU,CK,CRE,UA,TG,GOT,CHO,ALP,LDH,RBC,HGB,MCHC,PLT and WBC. Most hematological and biochemical parameters were also different by region difference. Conclusion The rearing condition and age difference were important influencing factors of these indexes.

Abstract:Objective To observe the effects of aspirin on insulin sensitivity in high fat fed rats. Methods Before and after ingesting aspirin, intraperitoneal insulin tolerance test was used to measure the insulin sensitivity, and fasting blood glucose, insulin and blood lipid were determined. Results Fat fed rats had similar glucose concentration to chow fed rats, but significantly higher levels of insulin, triglyceride(TG) and 1 h,1 5 h,2 h blood glucose concentration after intraperitoneal insulin tolerance test. Serum levels of insulin,TG and 1 h,1 5 h,2 h blood glucose concentration after intraperitoneal insulin tolerance test of fat fed rats treated with aspirin were significantly lowered. Conclusion It is confirmed that aspirin could raise insulin sensitivity of high fat fed rats.

Abstract:Objective To observe effect of pasiniazid gel against Mycobacterium tuberculosis in vitro and the safety of bronchial intervention therapy. Methods\ The MIC and MBC of pasiniazid and pasiniazid gel were measured by handwork and instrument methods,the safety of pasiniazid gel was assessed by bronchial intervention therapy using rabbits as experiment animals. Results\ The MIC of pasiniazid gel to M. tuberculosis H 37 Rv, M. bovis and M. phlei was 0 1, 0 1 and 0 4 mg/L; the MBC of those was 0 2, 0 2 and 1 6 mg/L respectively. The MIC and MBC of pasiniazid gel and pasiniazid had no significant difference without any harmful effect. Conclusion\ The effect of pasiniazid gel and pasiniazid against Mycobacterium tuberculosis was equivalent, and carbomer in the formula did not affect the activity of pasiniazid against Mycobacterium tuberculosis;meanwhile pasiniazid gel which contains carbomer was safe in bronchial intervention therapy.

Abstract:目的　研究不同分割液和分割前胚胎去致密化与否对昆明白系小鼠的桑椹胚和囊胚的徒手分割以及分割后胚胎移植的影响。方法　在mPBS、1 2 5 %蔗糖液和进口分割液三种不同分割液中对桑椹胚和囊胚进行徒手分割。结果和结论　在蔗糖液与进口分割液中分割桑椹胚 ,成功率显著高于mPBS处理组 (6 9 5 3%、77 4 0 %vs5 6 82 % ) (P <0 0 5 ) ,而半胚的囊胚发育率及囊胚细胞数三组差异均无显著性 (P >0 0 5 ) ;在蔗糖液与进口分割液中分割囊胚 ,分割后半胚培养的囊胚发育率显著高于mPBS处理组 (72 38%、74 2 9%vs 5 6 2 0 % ) (P <0 0 1 ) ,而分割成功率及囊胚细胞数三组差异均无显著性 (P >0 0 5 ) ;各处理组半胚的囊胚发育率及囊胚细胞数都显著低于对照组体外培养桑椹胚的囊胚发育率 (98 70 % )和囊胚细胞数 (6 3 6 7± 5 78) (P <0 0 1 ) ;桑椹胚经去致密化处理后分割 ,其分割成功率显著高于未处理组 (82 90 %vs 5 6 6 0 % ) (P <0 0 1 ) ,处理组半胚培养的囊胚发育率也显著高于未处理组 (73 80 %vs 4 6 80 % ) (P <0 0 1 ) ;共移植 1 4 2枚 2分胚形成的囊胚移植于 1 1只受体 ,其中 3只妊娠 ,分别产仔 2只、3只和 4只

Abstract:目的　动物的生长繁殖性能是其生理学数据的重要组成部分 ,对实验动物的开发和应用具有指导意义。方法　选择离乳的长爪沙鼠 80只 (雌雄各半 ) ,一雌一雄长期同居。结果　每胎产仔 3～ 7只的居多 ,占总胎数的 77% ;平均每胎产仔 (5 .0 2± 2 .1 1 )只 ;每年产仔胎数 5～ 9胎的较多 ,占总胎数的 91 .7% ,平均 (7.4 9± 2 .0 1 )胎 /年 ;最早的初产周龄为 1 3周龄 ,最长的 5 2周龄 ,在 1 3～ 2 0和 2 5～ 2 8周龄的居多 ,占总对数的 80 %。长爪沙鼠的平均出生重为 3.5g ,成年鼠平均体重雌性 5 5 .6g ,雄性 6 7.2g。结论　普通级长爪沙鼠封闭群配种日龄最早在 6 5日龄 ,初产日龄超过 2 0 0日龄的长爪沙鼠应淘汰 ;性别对长爪沙鼠体重无显著影响