Abstract:Objective To screen new microsatellite loci in Mongolian gerbils to develop genetic markers for genetic analysis.Methods 536 mouse loci were randomly selected from GenBank and amplified in Mongolian gerbils genetic DNA.The positive PCR products were sequenced and confirmed as simple sequence repeat(SSR) loci.Result Of these 536 mouse markers,313(58.39%) were discretely amplified from the laboratory gerbils.Of these 313 sequenced markers,130 were confirmed as SSR loci in the gerbil,in which 105 loci(80.77%) ...

Abstract:目的探讨罗格列酮(rosiglitazone,Ros)和血清脂(serum lipid,Lip)对绵羊前体脂肪细胞分化的影响及不同组织来源的前体脂肪细胞分化影响的差异。方法用不同浓度的Ros和(或)Lip培养绵羊皮下前体脂肪细胞和肾周前体脂肪细胞,通过测量3-磷酸甘油脱氢酶(GPDH)活性和油红O染色萃取液A值分析前体脂肪细胞的分化程度和脂肪细胞充脂量的变化,应用实时荧光定量PCR检测PPARγ和LPL mRNA的表达水平。结果 Ros和Lip提高细胞GPDH活性和脂滴的沉积量(P<0.05),上调LPL mRNA表达(P<0.05),最佳浓度分别为100nmol/L和20μL/mL;最佳浓度条件下Ros的诱导作用强于Lip(P<0.05),Ros显著提高了PPARγmRNA表达量(P<0.05),而Lip对PPARγmRNA的表达没有明显影响(P>0.05);Ros和Lip共同诱导与Ros单独作用之间没有明显差异(P>0.05);在相同诱导分化条件下,皮下前体脂肪细胞的分化程度高于肾周前体脂肪细胞(P<0.05)。结论研究结果表明Ros和Lip可促进绵羊前体脂肪细胞的分化,在相同条件下,皮下前体脂肪细胞的分...

Abstract:Objective To explore a technique to improve the efficacy of generation of transgenic sheep by injection of lentivirus into the perivitelline space of oocytes.Methods Recombinant EGFP lentivirus was injected into the perivi-telline space of oocytes.The growth status and gene expression of oocytes at different stages of embryonic development were assessed to analyze whether the injection doses of the lentivirus or the skill of different operators influence the embryonic de-velopment.Results(1) The lentivirus ...

Abstract:Objective To establish a model of hepatic fibrosis in mice labeling bone marrow cells with green fluo-rescent protein(GFP).Methods Thirty-two ICR mice were randomly divided into normal group and transplantation group.The mice in the transplantation group were lethally irradiated and received bone marrow transplants from GFP trans-genic ICR mice.The normal group was not irradiated and transplanted.Blood smear was prepared and examined after two months.The hematopoietic reconstitution animals were further div...

Abstract:目的 探讨miR-106b在阿尔茨海默病(Alzheimer's disease,AD)发病中的作用.方法 取3月龄和6月龄APPswe/PSΔE9小鼠脑组织,进行microRNA芯片的检测;利用real-time PCR检测3、6、9月龄APPswe/PSΔE9小鼠脑组织中miR-106b的表达,对芯片检测结果进行验证;通过构建miR-106b稳定转染细胞系和miR-106b knockdown研究miR-106b与TGFBR2表达之间的关系; 构建TGFBR2 3'UTR-荧光素酶报告载体,验证miR-106b是否可以直接调控TGFBR2蛋白的表达;采用Western blot的方法检测APPswe/ΔPSΔE9小鼠和对照小鼠脑组织中TGFBR2蛋白的表达情况.结果 miR-106b在3月龄和6月龄AD模型小鼠脑组织中表达升高,在9月龄模型小鼠脑组织中表达降低;通过体外实验,我们发现miR-106b与TGFBR2蛋白的表达呈负相关;荧光素酶报告实验表明TGFBR2 3'UTR序列中包含miR-106b的结合位点;TGFBR2蛋白在3、6、9、12月龄AD模型小鼠脑组织中表达均降低.结论 miR-106b可能通过调控TGFBR2蛋白的表达影响TGF-β信号通路,从而参与AD的发病.

Abstract:Objective To establish an experimental rabbit model for studying the effect of the continuous compres-sive stress on reconstruction of a large bone defect.Methods Twenty adult Japanese rabbits were used and randomly di-vided into two groups.Artificial bone made of foam silicon carbide was implanted in a large bone defect in length of 13 mm at mid-distal humeral shaft of right side.Ni-Ti shape memory connector with both fixation and continuous compressive stress was used as the experimental group,and that wi...

Abstract:Objective To explore the effects of berberine on the weight,blood glucose,lipid metabolism and ser-um insulin in KKAy mice and to investigate its possible glucose-lowing and lipid-regulating mechanism.Methods KKAy mice were randomly divided into two groups: berberine group(treated with 250 mg /kg /d berberine,n = 8) and control group(n = 8).The fasting blood glucose,weight,TC,TG,HDL-c,LDL-c and fasting serum insulin were tested.The OGTT test was done.RT PCR array experiment was done using skeletal muscle of...

Abstract:Objective To establish a rat model of chronic renal failure in two ways and observe the leptin expres-sion in the renal tissue of the rats.Methods 1.Chronic renal failure was induced by 5 /6 nephrectomy(Platt Method).2.Rats were fed with 2.5% adenine to cause chronic renal failure.The changes of blood BUN,Ca2 +,P5 + and Scr were as-sayed.HE and Masson staining were used for pathological examination,and immunofluorescence microscopy was used to ob-serve the leptin expression in the renal tissue of the model ...

Abstract:Objective To establish a rat model of abdominal heterotopic working-heart transplantation.Methods Thirty Wistar rats were obtained from Shanghai SLAC Laboratory Animal Company.Heterotopic hearts were transplanted u-sing end-to-side anastomosis between the donor's pulmonary artery and the donor's left atrium,and between the donor's as-cending aorta and the recipient's abdominal aorta.Results Fifteen heterotopic working-heart transplantation models were performed and 11 rats survived.The mean ischemic time wa...

Abstract:Objective To explore a suitable culture environment for tissue-engineered teeth and to evaluate the developmental ability of full tooth germs,dental papilla and enamel organ in sub-renal capsule space.Methods Rat fetus at embryonic day 18(ED18) were obtained by Cesarean section.The full first molar germs were separated from fetus,and to separate them further into enamel organ and dental papilla by microsurgery.Subsequently,full tooth germ,enamel organ and dental papilla were transplanted into sub-renal caps...

Abstract:目的 探讨GnRH-A激动剂(阿拉瑞林)主动免疫对垂体GnRHR基因表达的影响和生物信息学特性.方法 30只日本大耳白兔(Oryctolagus cuniculus)随机均分为三组,在EG-Ⅰ和EG-Ⅱ皮下注射1.0 mL(100 μg/mL)阿拉瑞林抗原,EG-Ⅱ于20 d 以原剂量加强免疫1次;从兔垂体中提取总RNA,PCR扩增GnRHR基因,进行反转录、克隆和测序;实时荧光定量PCR分析垂体中GnRHR、FSH-β和LH-β mRNA的表达,用DNAman、Tmpred、Signal P 3.0、Target P 1.1、Expasy、PSORT II prediction等生物信息学分析软件和在线工具,对GnRHR序列及其蛋白的理化特性、跨膜结构、信号肽和二级结构等进行分析与预测.结果 ①雄兔GnRHR的核苷酸为1179 bp,同源性达96%.ssDNA分子量362.66×103,dsDNA 726.78×103.②GnRHR二级结构中151(40.27%)个氨基酸组成α-螺旋,15(4.00%)个氨基酸组成β-折叠.③GnRHR由389个氨基酸组成,蛋白质的序列长度为375;理论等电点(pI)5.02,不稳定指数58.08,脂肪指数28.67,疏水性平均值(GRAVY) 0.869,表明该蛋白为不稳定的疏水性蛋白.④GnRHR蛋白TM螺旋长度为17～23,有34个强跨膜螺旋区,从内到外有35个螺旋.⑤GnRHR信号肽的概率0.999,最可能的酶切部位在17和18位点.结论 阿拉瑞林免疫可以明显降低垂体GnRHR、FSH-β和LH-β基因表达,加强免疫效果更佳.GnRHR是一种含有信号肽序列的不稳定的疏水性跨膜蛋白,具有明显的生物信息学特征.

Abstract:Objective To estimate the genetic diversity and genetic differentiation of a Dunkin Hartley guinea pig outbred stock and providing valuable information and references for standardization of the population.Methods A total of 72 samples from a Dunkin Hartley guinea outbred pig stock were genotyped using 8 microsatellite DNA markers by PCR-de-naturing polyacrylamide gel electrophoresis.Genetic variation of the population was evaluated and Hardy-Weinberg equilib-rium was tested for each locus.Results 28 alleles...

Abstract:Objective To investigate the feasibility of hemi-thyroid electrocoagulation in the establishment of a rat model of subclinical hypothyroidism(SCH).Methods Thirty-six young Wistar rats were randomly divided into three groups including sham,subclinical hypothyroidism,and clinical hypothyroidism(CH) groups.The rats in SCH group were given hemi-thyroid electriocoagulation,rats in CH group were given total thyroid electrocoagulation,while rats in the sham group only exposed thyroid without electrocoagulation.2 w...

Abstract:目的 探讨心肌缺血-再灌注损伤中趋化因子CXCL10的产生机制.方法 分别用LPS、H2O2、Ca2+载体A23187刺激原代培养的心肌细胞、骨髓来源的巨噬细胞或二者混合培养的共培养系统后,ELISA检测培养基上清中的趋化因子CXCL10和促炎性细胞因子IL-1β、IL-6、TNF-α的含量,观察其表达动力学.结果 ①大剂量(10 μg/mL)的LPS刺激心肌细胞主要产生趋化因子CXCL10;刺激骨髓来源巨噬细胞主要产生促炎性细胞因子IL-1β、IL-6、TNF-α.②H2O2、Ca2+通道激活剂并不能使产生趋化因子CXCL10或IL-1β、IL-6、TNF-α这些促炎性细胞因子.③骨髓来源的巨噬细胞促进心肌细胞表达趋化因子CXCL10;心肌细胞促进骨髓来源的巨噬细胞表达IL-6、TNF-α,但抑制IL-1β的表达.结论 心肌细胞是心肌缺血-再灌注损伤中CXCL10潜在的细胞来源;CXCL10的表达,主要依赖于TLR4的激活.

Abstract:目的 对长型瘦素受体在雌性昆明鼠生殖周期中的表达情况进行研究.方法 采用蛋白免疫印迹实验方法对下丘脑、胃、十二指肠组织中的瘦素受体进行了分析,并用免疫组织化学染色法对雌性KM鼠生殖周期不同阶段的下丘脑、胃、十二指肠组织中表达的瘦素长型受体进行定位分析.结果 与结论瘦素受体六个亚型的分子量大约分别为(120、90、77、66.2、54、47)×103;下丘脑神经元胞质中有棕褐色阳性颗粒,且数目随妊娠日龄增加而增加;胃底腺中下部分胞质和细胞核有棕褐色阳性颗粒,且阳性率随妊娠日龄的增加而增加;十二指肠腺中胞质和细胞核有棕褐色阳性颗粒,且阳性率随妊娠日龄的增加而增加.

Abstract:Objective To assess the effect of transportation stress on autonomic nervous function in beagle dogs based on heart rate variability(HRV) analysis,and define its recovery.Methods Sixteen beagle dogs were randomly di-vided into two groups(n = 8 in each): the control group and transportation stress group.Using a non-invasive telemetry system in conscious and unrestrained beagle dogs,electrocardiograms(ECG) were recorded in both groups after a 4 h transportation stress and after a recovery of 1,2 and 3 weeks.H...

Abstract:Objective To explore the optimal dosage of 2-deoxy-glucose(2-DG) in the establishment of a mouse model of endoplasmic reticulum stress.Methodes 108 healthy male SD rats were choosen and randomly divided into 6 groups: sham-operation group,ischemia-reperfusion group,2-DG 50 mg /kg group,2-DG 100 mg /kg group,2-DG 150 mg /kg group,2-DG 200 mg /kg group,using different dosage of 2-DG to set up models of endoplasmic reticulum stress.2-DG was dissolved in double distilled water on working concentration of 50 mg ...

Abstract:Objective To explore the effects of multi-intervention RAAS on ventricular remodeling and serum K+ concentration in the chronic pressure overload heart due to cardiac insufficiency in rats.Methods To establish chronic cardiac insufficiency animal models with suprarenal abdominal aortic banding in rats.Six-week old female Sprague-Dawley rats were enrolled and the suprarenal abdominal aorta was ligated by 4-0 nylon suture against a needle(outer diameter 0.7 mm).Twenty female rats were randomized into four gro...

Abstract:Huntington's disease(HD) is an autosomal dominant genetic disease characterized by dance-like invol-untary movements and progressive cognitive impairment.Excitatory toxicity is recognized as one of the mechanisms of HD pathogenesis.Quinolinic acid(QA) is an excitotoxic drug,which can lead to brain dysfunction by killing the related neu-rons in the brain,and being a further mimic of changes in HD.This article presents the merits and drawbacks of animal HD models induced by QA as well as the current applicati...

Abstract:Objective To establish a mouse model of hypoxic pulmonary hypertension and to explore the expres-sion of proliferating cell nuclear antigen(PCNA) in aorta and pulmonary artery smooth muscle cells of rats with hypoxic pulmonary hypertension.Methods SPF SD mice were randomly divided into 2 groups: normal group(n = 10) and hypox-ia group(n = 10).The mouse model of hypoxic pulmonary hypertension was constructed by intermittent ordinary pressure hypoxia.Histology of HE stained lung tissue sections and image anal...