Abstract:目的 研究二氢卟吩e6(Ce6)在移植瘤小鼠体内吸收、分布及代谢的动态变化,以期为声动力疗法处理不同部位肿瘤的时间点提供科学依据.方法 艾氏移植瘤小鼠尾静脉注射Ce6后,利用荧光分光光度法和小动物活体成像技术测定Ce6在小鼠不同组织的富集分布变化规律.结果 小鼠尾静脉给药后,Ce6迅速分布于全身各组织,在2 h内,各组织药物浓度均达到峰值,其中以肝含量最高.随后各组织中药物浓度均开始下降,以肝中清除速度最快.肿瘤组织中的Ce6含量在注射后不断上升,2 h时达到最高,随后开始下降,2～10 h代谢比较缓慢,24 h时浓度降至最低,但仍高于其他组织.结论 Ce6在艾氏移植瘤中具有肿瘤组织选择性好、潴留时间长并可迅速从体内排出等优点,有着很好的临床应用前景,同时提出了不同组织类型不同部位的肿瘤应根据各自适当的时间点进行处理.

Abstract:Objective To investigate the influence of Exo-1 gene deletion on the engraftment of hematopoietic stem cell(HSC) transplantation by different methods into the telomerase knockout mice.Methods Bone marrow cells or KSL((c-kit +,Sca-1 +,lineage-) cells were isolated from CD45.1 mice,and then injected into recipients,which were telomer-ase gene knockout or Exo-1 and telomerase gene double knockout mice with or without irradiation.At 1 month later,pe-ripheral blood was collected and analyzed by flow cytometry.Re...

Abstract:目的 探讨雌激素受体α(ERα)基因敲除小鼠的优化繁育方法及ERα基因敲除小鼠子代鼠的鉴定方法,建立ERα基因敲除小鼠模型,为进一步研究ERα蛋白的功能奠定基础.方法 用4种不同的交配方式观察子代鼠的各表型比率及雌、雄性ERα基因突变纯合子小鼠的繁殖能力;从子鼠鼠尾中提取基因组DNA,用PCR方法扩增ERα基因片段,琼脂糖凝胶电泳后观察结果.HE染色观察雌、雄性ERα<'-/->小鼠生殖系统表型变化.结果 WT、ERα<'+/->、ERα<'-/->各表型小鼠互交繁殖结果基本符合孟德尔遗传规律,且雌、雄性ERα<'-/->小鼠无繁殖能力.与WT比较,雄性ERα<'-/->小鼠睾丸脏器系数降低,睾丸病理变化表现为生精小管管腔膨胀,生精细胞层变薄,且排列不规则;雌性ERα<'-/->小鼠子宫脏器系数降低,子宫和卵巢病变明显,表现为:子宫浆膜、肌层、内膜层细胞排列不规则,卵巢有囊性病变、充血,无黄体.结论 雌、雄性ERα<'+/->小鼠交配是繁育ERα<'-/->小鼠的较好方法;实验所用PCR方法能够精确鉴定ERα<'-/->小鼠,ERα<'-/->小鼠的获得为ERα蛋白功能的实验研究提供了较理想的动物模型.

Abstract:Objective To establish a manipulation-easy animal model of post-ischemic myocardial fibrosis and in-vestigate the mechanism of collagen deposition.Methods Twenty BALB /c mice were randomly divided into two groups: the experimental group and control group.The mice in the experimental group were subjected to hypodermic injection with isoproterenol in a dose of 50 mg /kg.The injection was carried out twice a day for consecutive 10 days.Physiological saline was injected with the same method in the control group...

Abstract:Objective To explore the correlation of the hairless nature of Uncv hairless mice with the hairless gene(hr).Methods Based on the nucleotide sequence of hr gene of mice published in GenBank,five pairs of primers were designed and the CDS sequence of the hr gene of Uncv mice were cloned by RT-PCR.Result A full-length CDS se-quences(3546 bp) of the hr gene of Uncv mice and wide-type BALB /c mice were obtained.The length and sequence of hr gene of Uncv mice were fully consistent with that of BALB /c mice,and t...

Abstract:Objective To investigate the urinary metabonomic variation in diabetic rats in order to explore the ap-plication of metabonomics in research of diabetes.Methods Male Sprague-Dawley rats were fed with high-fat and high-su-crose diet for 6 weeks and then injected with vehicle or streptozotocin(STZ) in a dose of 37 mg /kg.Fasting blood glucose(FBG) was monitored weekly after STZ injection.Urines of normal rats and model rats were collected at 1,2,4,6,8 weeks after STZ injection.Serum insulin(INS),triglyceride(...

Abstract:Objective To study the nutrition approach for lumbar intervertebral disc cartilage in normal goat.Methods Eight 24-month-old healthy adult goats were included in this study.There were four lumbar intervertebral discs for each goat,and there were a total of 32 lumbar intervertebral discs.After anesthesia of the goats,The signal changes of regions of interest were observed by dynamic contrast-enhanced magnetic resonance scan.On the time points before en-hancement,and 0 min,5 min,10 min,30 min,1 h,1.5 h,2 h,2....

Abstract:Objective To investigate the effect of curcumin on bronchopulmonary dysplasia induced by hyperoxia in neonatal rats and explore its potential mechanism.Methods Eighty SD newborn rats were randomly divided into 4 groups: room air group,hyperoxia group,hyperoxia + vehicle group,hyperoxia + curcumin group.Pathomorphology of the lungs was observed with hematoxylin-eosine staining.Cell apoptosis in the lung tissue was detected by TUNEL staining.The expression of activated caspase-3 was detected by immunohistoche...

Abstract:目的了解无菌级C3H/OdSlac小鼠的生长、繁殖性能;测定无菌级C3H/OrlSlac小鼠主要脏器重量以及血液生理、生化正常值并进行分析比较.方法 ①统计无菌级C3H/OrlSlae小鼠的1～3胎的繁殖指标数据,包括:平均每胎产子数、离乳率、怀孕率、胎间隔和生产指数;②分别称取60只(雌雄各半)0～112 d的无菌级C3H/OrlSlac小鼠体重,绘制其生长曲线;③随机抽取28、56、112日龄的C3H/OrlSlac小鼠60只(雌雄各半),活体称重,依次剖取心、肝等主要脏器称重;④小鼠眼眶采血,测定其血常规和生化值.结果 无菌级C3H/OrlSlac小鼠的平均胎间隔为(27.7±3.5)d,平均产子数为(6.0±1.3)只,平均怀孕率为(98.1±4.7)%,平均离乳率为(95.2±3.2)%,平均生产指数为(5.3±0.8).1～3胎间差异无显著性(P>0.05).无菌级C3H/OrlSlae小鼠的体重随着日龄的增长而逐渐增加,断奶后1～2周内增重最为迅速,6周后的体重雌雄间出现明显的差异(P<0.05).主要脏器(心、肝、脾、肺、脑等)重量随年龄增大均逐渐增加,而胸腺随着日龄的增大而逐渐萎缩.其重量迅速减轻.无菌级C3H/OrlSIac小鼠的血常规除56日龄的雌雄间无差异外.同一日龄的雌雄间血常规和生化值差异均有显著性(P<0.05).结论 无菌C3H/OrlSlac小鼠体重和主要脏器重量的数据可反映其结构变化以及生理功能状态;性别和年龄都会影响无菌级C3H/OrlSlac小鼠的血液和生化值指标.

Abstract:Objective To establish and evaluate the feasibility of a rat model of chronic obstructive pulmonary dis-ease(COPD) induced by intratracheal instillation of lipopolysaccharide(LPS).Methods LPS or saline was instilled in-tratracheally in rats once a week for consecutive 8 weeks.The rats were anesthetized and taken for the measurement of lung resistance(RL) and dynamic compliance(Cdyn).Total and differential white blood cell counts in bronchoalveolar lavage fluid(BALF) were carried out.The pathological changes...

Abstract:Objective To evaluate the mRNA expression abundance of genes,which are related with embryonic development and ability to undergo successful fertilization,cleavage and blastocyst development,in the GV stage and in vitro matured(IVM) bovine oocytes.Methods The oocytes that vitrified-warmed at GV(n = 224) and IVM(n = 235) were in vitro fertilized and cultured up to blastocyst stage under standard conditions.In parallel,we analyzed the relative a-bundance of Bax,Dnmt1,Hdac1,cyclinb1,Cdc2 and Cu Zn SOD transcrip...

Abstract:目的 研究哺乳期接触双酚A(BPA)对雄性小鼠睾丸结构及雌激素受体β(ERβ)表达的影响.方法 20只妊娠母鼠,待分娩后随机分为高、中、低剂量组,BPA染毒剂量为100、50、5 ms/(kg·d)和对照组,母鼠分娩后第2天开始每天灌服BPA,仔鼠通过乳汁暴露于BPA,一直持续到第22天断奶,研究其成年后睾丸组织形态学改变.并用免疫组织化学、RT-PCR和Western blot方法研究BPA对其成年后睾丸内ERβ表达的影响.结果 睾丸形态学分析表明BPA处理组睾丸发育受到严重影响,生精细胞排列紊乱,支持细胞和各级生精细胞生长受到不同程度的抑制;免疫组化、RT-PCR及Western blot均显示,高、中、低剂量组雄鼠成年后睾丸组织中ERβ的表达均高于对照组,差异均有统计学意义(P<0.05,P<0.01).结论 哺乳期接触BPA影响雄鼠睾丸发育,改变其成年后睾丸形态,提高睾丸组织内ERβ表达,提示BPA可能通过改变雌激素受体表达从而影响睾丸发育.

Abstract:Objective To establish a database of the physiological,biochemical and pathological indexes of the green fluorescent protein(GFP) transgenic mice and provide theoretical evidence for toxicological and other studies.Meth-ods Two hundred and forty one GFP mice and 100 C57BL /6J mice were included in this study.These mice were of 4-6-week-old,6-8-week-old,9-12-week-old,and 13-14-week-old.The body weight and food intake of the mice were recorded regularly.The blood routine examination was performed and blood bi...

Abstract:Objective To explore a more effective method of isolation of adult rat cardiomyocytes.Methods Car-diomyocytes from SD rats were isolated by collagenase type II perfusion in the control group,and by pressure perfusion with collagenase type II in the experimental group.The morphology of cardiomyocytes was observed by optical microscopy and the cardiomyocyte viability was evaluated by trypan blue exclusion test and local field stimulation.Results The ratio of rod-shaped cells and yield of viable cells per rat ...

Abstract:目的 快速、灵敏、可靠的检测与临床多种疾病密切相关的重要基因TNF-α的表达情况,构建TNF-α基因及内参GAPDH基因荧光定量PCR质粒标准品.方法 利用版纳微型猪近交系4～6月龄猪建立动物模型,提取皮肤创面总RNA,设计特异引物.进行RT-PCR扩增.纯化目的片段与pMD18-T载体连接,转化宿主菌DH5α,提取重组质粒DNA,并经酶切、PCR和测序鉴定,计算重组质粒原液拷贝数浓度并制备梯度浓度标准品,进行实时荧光定量PCR,生成标准曲线.结果 建立的TNF-α基因和GAPDH内参基因mRNA表达实时荧光定量PCR检测方法灵敏度分别可达10<'3>和10<'5>拷贝,线性范围分别为10<'3>～10<'9>和10<'5>～10<'9>拷贝,阈值循环数(Ct)与PCR体系中起始模板量的对数值之间存在的线性关系R<'2>分别为0.993和0.999,扩增效率E分别为111.073%和95.948%.结论 成功的构建了版纳微型猪近交系TNF-α基因质粒标准品和标准曲线,并用内参基因GAPDH进行校正,此方法可为探讨TNF-α基因在临床多种疾病中所发挥的分子机理奠定基础.

Abstract:The research on Yersinia pestis greatly focuses on the pathogenetic mechanism,vaccines and treatments.Effective animal models of plague are the basis of these researches.Numerous types of animal models of plague have been developed,including mice,guinea pigs and non-human primates,etc.,and they are useful for understanding,defending and treating this disease.Some classic models are still used at present for different purposes,however,others were discar-ded because of certain problems.The advantages and disa...

Abstract:At present,the Tupaia belangeri used for biomedical studies is mainly sourced from wild field capture.Due to the variation of interindividual differences,there are still some shortages in the homogeneity and the repeatability of experiments on those animals.There are problems demanding prompt solution on a series of basic scientific questions such as the management,critical techniques for breeding and the animal quality control standard in Tupaia belangeri.A lot of re-searches have reported that the Tupaia ...

Abstract:Objective To develop an application software for researchers to design comparative medical animal ex-periments as well as to learn the designing process independently.Methods The technical information of comparative medical animal researches were screened according to thescience,ethic,economic principle of laboratory animal us-age.The candidate data were analyzed and organized on the basis of relational database schema theory.The framework was designed and the modules were set up refered to the rules and fe...