Abstract:Objective To find a new source of human feeder cells which can support the undifferentiated growth of human embryonic stem cells.In this study,human umbilical cord mesenchymal stem cells(HUCMSCs) were tested as feeder cells for supporting the growth of hESCs to prevent the possibility of contamination from animal feeder cells.MethodsMesenchymal stem cells were isolated from human umbilical cord by collagenase IV digestion.Their surface markers were detected by flow cytometry.The cells were induced to differ...

Abstract:目的评价聚羟基脂肪酸(polyhydroxyalkanoates,PHA)、聚乳酸(polylacetic acid,PLA)和聚己内酯(polycaprolactone,PCL)三种膜性高分子材料在兔眼部的生物相容性。方法将24只新西兰兔随机分为4组,每组6只。PHA、PLA、PCL为实验组,材料植入兔右眼结膜下。假手术组结膜下钝性分离,但不植入任何高分子材料。使用裂隙灯显微镜观察并记录植入后不同时间手术眼的反应并评分。裂隙灯下观察材料的吸收时间。术后4周和16周取眼球,行HE染色、Masson染色和天狼猩红染色分别定性观察组织结构和炎症细胞、胶原纤维和胶原纤维的亚型与排列方向。结果术眼刺激性评分等级各组均不高于轻度刺激性。结膜下吸收时间PHA、PLA和PCL组分别是16周,12周和大于16周。组织学观察术后4周PHA、PLA和PCL组均形成材料包裹囊腔,囊壁以纤维组织为主,伴有毛细血管形成和炎性细胞浸润,以中性粒细胞为主。胶原纤维染色与假手术组无明显差异,以Ⅰ型和Ⅲ型为主,大致呈平行排列。术后16周PHA和PLA组材料已不可查及,包裹囊腔结构不规则,而PCL材料整体可查及,包裹囊腔规则。各组未见毛细血管,...

Abstract:Objective To investigate the effect of interleukin-4(IL-4) and interleukin-12(IL-12) on survival of purified retinal ganglion cells(RGCs),and to explore the association of autoimmune dysregulation with RGCs injury.Methods Mouse RGCs were isolated and purified using anti-mouse Thy1.2 monoclonal antibody(two steps of antibody adhesion).The purity of RGCs were verified by immunohistochemistry using double labeled Thy1.2 monoclonal antibody combined with microtubule-associated protein-2(MAP2) polyclonal antibod...

Abstract:目的研究Müller细胞在大鼠视网膜绿光损伤模型中的激活反应。方法 72只出生后8周(约230～280g)的成年雄性Sprague-Dawley大鼠随机分9组。一组作为正常对照,另8组暗适应24 h后置于波长为(530±10)nm的LED灯光箱中照射3 h,并分别于暗恢复3、6、12 h及1、2、3、7、15 d取眼球,光学显微镜下观察同一定位处视网膜组织形态、检测(TUNEL)凋亡细胞、免疫组织化学染色及蛋白免疫印迹分析。结果光损伤后光感受器内外节变短,外核层变薄,细胞排列紊乱;细胞凋亡出现在外核层,暗恢复3 h出现,3 d时达到高峰,7 d时消失;胶质细胞纤维酸性蛋白(glial fibrillary acidic protein,GFAP)表达量于暗恢复12 h开始升高,随暗恢复时间延长逐渐升高;谷氨酰胺合成酶(glutaminesynthetase,GS)总表达量未见明显改变,但可见蛋白一过性重分布,表达部位由内核层移至外核层。pSTAT3蛋白表达量于损伤后12 h出现一过性升高。结论绿光损伤视网膜激活Müller细胞,pSTAT3可能参与了此激活过程。

Abstract:目的观察氧诱导视网膜病模型(OIR)中曲安奈德(TA)对CD14+细胞聚集及VEGF表达的干预作用,初步探讨曲安奈德抑制视网膜新生血管生长的可能机制。方法清洁级C57BL/6哺乳期小鼠36只(共36个眼球),随机将其分为四组:①正常对照组(6个眼球),6只17日龄正常小鼠先行FFA眼底造影,然后每鼠随机摘取一个眼球,行眼球石蜡切片HE染色。②单纯高氧组(6个眼球),6只17日龄OIR模型小鼠处理同单纯对照组。③TA高氧组(12个眼球),12只12日龄OIR模型小鼠随机一眼注射TA 2μL,再于17日龄后行FFA眼底造影后摘除术眼,其中6个眼球行眼球石蜡切片HE染色及视网膜CD14和VEGF免疫组化染色,另6个眼球行视网膜VEGF mRNA的real-time PCR检测。④BSS高氧(12个眼球),12只12日龄OIR模型小鼠随机一眼注射BSS 2μL,余处理同TA高氧组。用t检验两两比较各组视网膜突破内界膜的内皮细胞核数,视网膜CD14与VEGF免疫组化染色的平均吸光度值(IOD/AOI),及VEGF mRNA的相对含量值(2-ΔCt×105)。结果与正常对照组相比,高氧诱导组突破视网膜内界膜的内皮细胞核数...

Abstract:目的研究不同浓度消旋山莨菪碱液对雏鸡形觉剥夺近视眼轴的影响。方法 3日龄雏鸡60只右眼遮盖半透明塑料膜,随机分为6组:给药组4组,不同浓度消旋山莨菪碱液(0.05%组,0.1%组,1%组,2.5%组),隔天行右眼球结膜下注射,每次给药50μL,共4次;对照组为生理盐水组,注射方法同给药组;单纯形觉剥夺组,不做干预。8 d后观察药物对眼轴的影响。结果 2.5%浓度组右眼与左眼眼轴差异显著小于低浓度组及对照组(P<0.05),给药组未发现组织病理毒性。结论消旋山莨菪碱对雏鸡近视眼轴增长有一定抑制作用,无毒副作用,有一定浓度依赖性。

Abstract:Objective To investigate whether exercise-training on a treadmill can induce physiological cardiac hypertrophy in mice,and to determine the difference between the physiological and pathological cardiac hypertrophy.Methods Forty wild-type male C57BL/6 mice were randomly divided into four groups(10 mice in each group): normal control,training group,sham control,and transverse aortic constriction(TAC) surgery group.After treadmill training for 40 days and at 2 weeks after TAC surgery,the heart function and str...

Abstract:Objective To understand the growth characteristics of bovine cumulus cells and the transfection efficiency of cumulus cells,based on the gene vector pGC1-collagen-EGFP-Neor that containing human collagen type I cDNA gene and mammary gland specific promoter,constructed in our laboratory.Methods Bovine cumulus cells were obtained by digestion of bovine cumulus/oocyte complexes(COCs) using hyaluronidase.Gene vector pMSCV-EGFP-Neor and pGC1-collagen-EGFP-Neor was transfected into cumulus cells with cationic lip...

Abstract:Objective To isolate microsatellite markers in tree shrews(Tupaia belangeri chinensis),and to fill the gap of lack of specific genetic markers of tree shrews.Methods A partial genomic library was constructed in tree shrews.Thirty-six positive clones were isolated from screening about 1500 clones of the genomic library with(CA)15 probe labelled at the 5'end with digoxin.Sequences of these clones and 15 microsatellites were isolated which included one repeated clones and one short flank sequence.Results The o...

Abstract:Objective To better understand the mechanism of transparency in adult crucian carp,explore its application and provide more evidence to support it as a novel aquatic animal model.Methods The transparent crucian carp was bred,and the phenotype of offsprings was examined to learn the inheritance of the transparent character.A stereomicroscope was used to examine the types and patterns of chromatophores in transparent and common crucian carps.The fish infected with microsporidia were closely monitored.Tissue s...

Abstract:目的建立小鼠结核分枝杆菌耐利福平株静脉感染小鼠模型。方法 30只BALB/c雌性小鼠经尾静脉感染结核分枝杆菌耐利福平株每只106CFU,观察小鼠一般状况,并分别在感染后6周、10周、14周处死小鼠,进行脾、肺组织病理切片、抗酸染色、计脏器荷菌数。结果感染后小鼠体重呈逐渐上升趋势,脏器病理变化随时间推移由急性炎症逐渐转为慢性炎症反应,抗酸染色均为阳性,脏器荷菌数与感染剂量相对应,并至少可持续14周。结论成功建立了小鼠结核分枝杆菌耐利福平株静脉感染动物模型,为其进一步用于结核病防治研究奠定了一定的基础。

Abstract:Objective To obtain the coding region sequence the SRY gene of Banna mini-pig inbred line(BMI) and construct the molecular phylogenetic tree.Methods The coding region of the SRY gene of BMI was cloned and sequenced by using housekeeping gene GAPDH as internal control,then based on the nucleotide and amino acid sequence to compare with some species(whale,dolphin,deer,sheep,cattle,seal,horse,walrus,panda,human,ass,bear,cat,tiger and leopard) by Lasergene,BioEdit,ClustalX,Mega bio-softs,and also constructed th...

Abstract:Objective To establish a model of retinal dehydrogenase type 2(raldh2) gene knock-down zebrafish embryos,and to explore the effect of insufficient retinoid acid(RA) signaling on the early embryonic cardiac development,especially on the regulation of cardiac development-related nppa and tbx20 gene expression.Methods A morpholino antisense oligonucleotides(MO) targeting zebrafish raldh2 gene was designed to knock down its expression and constructed a raldh2-EGFP plasmid to verify the effectiveness and specifi...

Abstract:Objective To observe the status of cerebral glucose metabolism in normal control group mice,APP/PS1 double transgenic mice of model group and curcumin treatment group by 18F-FDG microPET.Methods APP/PS1 dtg mice from the model group,curcumin treatment group,and normal C57BL/6J mice with the same background were randomly selected(age: 6 months;n=3)for microPET study.Each mouse was anesthetized using 2% isoflurane and was injected through a tail vein with 14.8-16.5 MBq 18F-FDG as a bolus.After 45-minute of 18...

Abstract:Objective To evaluate the inhibitory effect and toxicity of juglone administered via different routes on the nude mice model bearing subcutaneously transplanted tumor.Methods Human hepatocellular carcinoma BEL-7402 cells were subcutaneously injected into nude mice.The resultant tumors were taken and prepared into small tissue blocks,which were implanted subcutaneously into nude mice to establish mouse models bearing human hepatoma.Juglone was administered at different doses by intraperitoneal injection and ...

Abstract:Objective To screen the microsatellite loci of Chinese hamster DNA to serve the genetic studies of germplasm resources,classification and evolution of Chinese hamsters.Methods Genomic DNAs from Chinese hamster was fragmented by ultrasonication.The fragments in size from 500 bp to 1000 bp were recovered by 2% agarose gel electrophoresis and ligated to SNX linkers with T4 DNA ligase,then denatured and hybridized to 14 biotinylated oligonucleotides.The biotinylated hybrids were retained on magnetic beads accor...

Abstract:Objective To develop an easy and simple method of establishing mouse model of paraquat-induced pulmonary fibrosis.Methods Thirty-three C57BL/6J mice were divided randomly into the experimental group(n=30)and control group(n=3).Paraquat(10 mg/kg) was administrated to the animals of the experimental group by a single intraperitoneal injection.The mice were killed on the day 2,5,7,14 and 28 after paraquat administration.The mice in the control group were treated by an intraperitoneal injection of physiological...

Abstract:目的检测转化生长因子-β1(transforming growth factor-β1,TGF-β1)对体外培养大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cell,BMMSCs)增殖及Slug表达的影响。方法采用密度梯度离心结合贴壁法分离、培养大鼠BMMSCs,用免疫组织化学方法对培养第3代的细胞进行鉴定。用MTT法检测不同浓度TGF-β1对细胞增殖的影响;免疫荧光和免疫印迹法检测TGF-β1处理前后Slug的表达情况。结果密度梯度离心结合贴壁法能有效分离、纯化大鼠BMMSCs,免疫组织化学法检测显示CD29、CD44表达阳性,而CD34、CD45表达阴性;低浓度TGF-β1对BMMSCs的增殖有促进作用,高浓度却抑制BMMSCs的增殖。TGF-β1处理24 h,Slug蛋白表达明显增强。结论一定浓度的TGF-β1可以促进BMMSCs的增殖,而且引起Slug蛋白增加。

Abstract:Objective To amplify the resistin gene from adipose tissue of ICR mice for further research of its functions.Methods Total RNA was extracted from the adipose tissue for amplification of resistin gene by RT-PCR.The sequence of amplified nucleotide acids was analyzed by DNAMAN software after gel electrophoresis and sequencing.Results The total RNA had good integrity.The expected 363 bp fragment was obtained by RT-PCR.The base peak was uniform in both directions of sequencing.The amplified fragment showed a 93...

Abstract:大量研究证实帕金森患者黑质铁异常沉积,脑内铁代谢紊乱,铁促进氧化应激是神经细胞损伤的重要病理机制。本文综述了铁代谢异常与帕金森病关系的研究概况,指出从中药中寻找金属螯合活性成分治疗PD是极其诱人的研究方向。

Abstract: