Abstract:【Abstract】 Objective Establish a rat model of doxorubicin induced nephrotic syndrome and observe its dynamic development.Methods Twenty six male Wistar rats were randomly divided into model group and control group,the model group received a single tail intravenous injection of doxorubicin 6.2mg/kg body weight,the control group were injected with isotonic saline.12-hour urines 10 weeks in a row were collected and checked once a week,blood biochemical index was checked when the rats were killed,the kidney was observed under light and electron microscopy.Results Urinary protein excretion in the model group significantly increased(P <0.01) at 7th day,peaking at 5th week;serum total protein and albumin were decreased, triglyceride, cholesterol and blood urea nitrogen were increased(all P <0.05),serum creatinine was not significantly changed(P =0.64).Renal pathological changes:at 5th week minimal change disease was developed,at 10th week focal segmental glomerulosclerosis can be seen.Conclusion A single tail intravenous injection of doxorubicin 6.2mg/kg,can establish a progressive model of nephritic syndrome in rats.

Abstract:Objective To explore the effect of bone marrow mesenchymal stem cells (BMSCs) transplanted into the unilateral lesioned substantia nigra (SN), changes of postural asymmetry and expression of tyrosine hydroxylase in SN of Parkinson’s disease(PD) model rats, as well as the survival and differentiation of BMSCs. Methods 6-hydroxydopamine(6-OHDA) was injected into rat right SN and medial forebrain bundle, performed the behavioral test. Then the PD rats were randomly divided into transplant group and control group. The PD rats rotational behavior were induced by apomorphine (APO) after BMSCs transplanted into SN for 4 weeks and 8 weeks, and the survival and differentiation of BMSCs transplanted into SN of PD rats were detected by immunohistochemical and immunofluorescent methods. Results After BMSCs being transplanted into the SN of PD rats, the number of rotations reduced from 10.62?.97 r/min to 4.65?.08 r/min(P<0.01), while the number of positive TH cells in SN and the density of positive TH fibers in striatum were increased significantly. The transplanted BMSCs in PD rats can survival at least 8 weeks, and part of cells could differentiate to neural stem cells, neurons and glial cells. Conclusion Transplanted BMSCs into SN of PD rats may have therapeutic effects for PD model rats.

Abstract:Objective To compare the different role of 5 behavioral tests in amotrophic lateral sclerosis transgenic mouse Methods This study was divided into model group (SOD1-G93A mice) and negative control group (littermates neg ative control). Behavior changes were evaluated by 5 behavioral assessments (Assessment of general condition, weighing, rotarod test, grip strength measurement and step length analysis). Results (1) Assessment of general condition: The general condition of the model group began to decline at day 89. In day 101, the differences between negative control group and model group were significant for the first time, P=0.000. (2) Weighing: Weighing of the model group began to decline at week 15 (day 105), and the differences between negative control group and model group were significant for the first time, P=0.026. (3) Rotarod test: The time of rotarod test began to decline at week 11(day 77). In week 13 (day 91), the differences between negative control group and model group were significant for the first time, P=0.047. (4) Grip strength measurement: Grip strength for hindlimb began to decline at week 10 (day 70). In week 13 (day 91), the differences between negative control group and model group were significant for the first time, P=0.000. (5) Step length analysis: Step length for hindlimb began to decline at week 14 (day 98). In week 15 (day 105), the differences between negative control group and model group were significant for the first time, P=0.000. Conclusion Grip strength measurement is better than others in behavioral tests of the SOD1-G93A mice.

Abstract:objective To study the effects of large ionizing radiation exposure on murine lung tissues . Methods The mice was exposed to 60Co γ ray at 16 Gy. The pulmonary injury was observed after hematoxylin-eosin staining and the expression levels of TGFβ1 and ICAM1 were detected with immunohistochemistry . Results The murine lung showed evident injury three days after large ionizing radiation exposure and the expression levels of TGF-β1 and ICAM1 increased significantly. Conclusion The endothelial cell injury and the intravascular material leakage would be the early important events，and early detection of ICAM1 might be helpful to predict the severity of acute radioactive lung injury.

Abstract:Objective: To bulid the methodology of acute animal model of atrial fibrillation (AF) induced by increased sympathetic nerve activity. Methods: Sixteen mongrel dogs weighing 18 to 25kg were divided into 3 groups. Control group (n=4) underwent 6-hour rapid atial pacing only. Right stellate ganglion(RSG) group (n=6) and Left stellate ganglion(LSG) group (n=6). AF induction rate, and AF duration in left atrium(LA), right atrium(RA), left superior pulmonary vein(LSPV) and left inferior pulmonary vein(LIPV) sites were measured. Results: (1) The effect on AF inducibility: In RSG group, the induction rate of AF was significantly increased in RA sites(P＜0.05), compared with baseline. However, there was no significant changes in LA, LSPV and LIPV sites. In LSG group, the induction rate of AF was significantly increased (P＜0.05), compared with baseline in LA, LSPV and LIPV respectively. Compared with RSG stimulation, right stellate ganglionectomy can markly decrease AF induction rate of RA(P＜0.05). Compared with LSG stimulation, left stellate ganglionectomy can markly decreased AF induction rate of LA, LSPV and LIPV(P＜0.05). (2) The effect on AF duration: In RSG group, the duration of AF was significantly prolonged in RA sites(P＜0.05), compared with baseline. In LSG group, the duration of AF was significantly prolonged(P＜0.05), compared with baseline in LA, LSPV and LIPV respectively. Compared with RSG stimulation, right stellate ganglionectomy can markly shorten AF duration of RA(P＜0.05). Compared with LSG stimulation, left stellate ganglionectomy can markly shorten AF duration of LA, LSPV and LIPV(P＜0.05). Conclusion: Unilateral stellate ganglion electrical stimulation puls rapid atial pacing for 6 hours can successfully establish canine model of acute AF mediated by sympathetic nerve. Stellate ganglion stimulation promote AF induction and prolong AF maintenance in atrial and pulmonary sites. The inhibition sympathetic nerve activation by unilateral stellate ganglionectomy can reduce the AF initiating and sustaining.

Abstract:Murine Hepatitis Virus（MHV）is a corona RNA virus, usually subclinical infection. The quality of the experimental animals and the results of animal experiments are affected. The epidemic scope can be controlled and the impact on the results of animal experiments can be prevented by timely detection of MHV. This article reviews MHV detection and diagnosis methods in recent years, analyzed MHV infection in experimental animals and animal experiments, and analyzed the pros and cons of those detection methods to provide a theoretical basis for further research.