Abstract:Objective The effect of acute perfluorooctane sulfonate (PFOS) exposure on the induction of oxidative stress was studied in the livers of swordtails (Xiphophorus helleri Heckel) in order to explore the toxic mechanism of PFOS in fish. Methods Swordtails were randomly divided into five groups, with one control group and four experimental groups (3.5, 7.0, 14.0, and 28.0 mg/l). Parallel experimental groups were also established. The oxidative stress index (malondialdehyde aldehyde, MDA) and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX) activity in fish livers were determined after 12 h, 24 h, 48 h, and 96 h exposure. Results After 12 h exposure to PFOS, the 28.0 mg/l exposure group exhibited significant inhibition in SOD activity. However, in comparison with the control group, there were no significant differences in the other three groups（p > 0.05）. SOD activity was significantly induced in two exposure groups (7.0 and 14.0 mg/l) after 24 h exposure and this upward trend was maintained to 96 h. SOD activity increased during the early hours of exposure and reached a maximal value at 24 h, at which point there was a significant difference with the control group, before decreasing gradually and eventually resuming an upward trend at 96 h. This pattern suggests that such activity may be related to PFOS in the biological body through the elimination mechanism of enterohepatic circulation. Over the same timeframe, CAT activity decreased as the concentration of PFOS increased. Three groups (7.0, 14.0, and 28.0 mg/l) were significantly inhibited at 12 h. CAT activity in each group exhibited slight upward trend, but remained lower than the level observed in the control group after 24 h exposure. After 48 h, each group showed a decreasing trend that continued until 96 h and their CAT activity returned to the level recorded at 12 h. Although the changing trends in GSH-PX and CAT activity were observed to be similar, GSH-PX activity in the 28 mg/l exposure group exhibited significant inhibition at each time point. MDA content decreased slightly at 12 h exposure, indicating the influence of bio-metabolic changes or other physiological factors rather than oxidative stress. However, as the exposure time lengthened, the MDA content of each group continued to increase and reached a maximal value at 96 h. Conclusions The results of this study show that a relative high concentration of PFOS has an apparent effect on antioxidant enzyme activities in swordtail livers. The swordtail in vivo experiments undertaken therefore show that PFOS could induce oxidative stress in the liver and that PFOS-induced oxidative damage is a major route for this toxic effect.

Abstract:Abstract: Objective To analyzed the genetic background of Atp11c gene trap mice. Methods The entire region PCR, was applied to find the gene trap vector insertion site and the lack state of the host chromosome.The fluorescent competitive PCR technology was used to detect the copy numbers of the integrated vector(s). Results The PCR results by 54 pairs of primers showed the trapping vector insertion site was in the first intron of,Atp11c gene, and the DNA sequencing results displayed 418 bp deletion on the host chromosome and more than 200bp missing on both ends of the vector. Fluorescent competitive PCR confirmed a single copy trapping vector inserted.Conclusion The genetic background of Atp11c gene trap have been resolved successfully and a program has been established to detect the genetic background of gene trap mice accurately and quickly.

Abstract:【Abstract】 0bjective To explore the effects of sarcoendoplasmic reticulum calcium ATPase2a(SERCA2a) overexpression on the protein expression of L-type voltage dependent calcium channel (LVDCCalc) subunit in the rabbit model of atrial fibrillation(AF). Methods Forty-eight New Zealand rabbits were randomized into four groups (n=12),including control group,AFgroup,rAAV9 EGFP AFgroupand rAAV9 SERCA2a EGFP AF group.rAAV9-EGFP and rAAV9-SERCA2a-EGFP(1.0?012v.g/礚 for each animal )were injected into pericardium of the animals of rAAV9 EGFP AF and rAAV9 SERCA2a (enhanced green fluorescent protein)EGFP AF groups before rapid atrial pacing thirty day. Thirty day after gene transfection,AF was induced by rapid atrial pacing(600 beats／min)for 24 hours in each animal of AF group,rAAV9 EGFP AF group and rAAV9 SERCA2a EGFP AF group .Each animal of control group was not paced,Animals were scarified to observe expression of the green fluorescent protein in the myocardial frozen section of rabbits in rAAV9 EGFP AF group under the inverted fluorescence microscope,to assess the right atrial tissue morphology and detect the expressions of SERCA2a protein and LVDCCalc protein in the right atrial tissue in each group by H E staining and immunohistochemistry at 24 hours after pacing. Results Compared with AF group and rAAV9 EGFP AF group(P<0.05),expressions of SERCA2a protein and LVDCCalc protein in the right atrium of rabbits in rAAV9 SERCA2a EGFP AF group was significantly upregulated,but there is no difference on expressions of SERCA2a protein and LVDCCalc protein in the right atrium of rabbit among rAAV9 SERCA2a EGFP AF group,AF group and rAAV9 EGFP AF group (P>0.05). Conclusions The atrial tissue transferred SERCA2a protein could significantly decrease the downregulation of the LVDCCalc protein expression in this rabbit model of AF and reverse acute AF electrical remodeling.

Abstract:Objective To investigate the effects of high-fat diet rich in n-3 or n-6PUFA on the expressions of Srebp2 and Hmgcr in SD rat. Methods Normal male SD rats were randomly divided into 3 groups, including normal control group (NC), high n-3PUFA formula diet group (TUF) and high n-6PUFA formula diet group (SUF). After 8 weeks, serum total cholesterol (TCH) was measured. Srebp2 and Hmgcr gene expressions were detected by quantitative real-time PCR and the proteins were observed by Western blotting. Results Serum TCH in TUF group and SUF group were both significantly lower than the NC group (P <0.001), TUF group was the lowest. Meanwhile, gene expressions of Srebp2 and Hmgcr in TUF group and SUF group were both significantly higher than the NC group (P =0.007, P =0.012). According to Western blotting, HMGCR in the TUF group is higher than NC and SUF, while it wasn’t significantly different between SUF and NC group; as to SREBP2, there wasn’t significant difference of the three. Conclusions Overfeeding of n-3 or n-6PUFA reduces rat’s serum TCH, which is not resulted from the expressions of the Srebp2 and Hmgcr.

Abstract:【Abtract】 Objective Observe the therapeutic effect of Crucian carp decoction on rats with nephrosis induced by adriamycin, and explore the mechanism. Methods Fourty male Wistar rats were randomly divided into Crucian carp decoction group、Irbesartan group、Nephrosis group，and Control group. Nephrosis of rats were induced by a single tail intravenous injection of adriamycin 6.2mg/kg body weight.The rats were administered once oral gavage of Crucian carp decoction group for 9 weeks, urinary protein was checked each week.All rats were killed at week 9.Histological changes were observed by light microscopy and blood biochemical indices were detected. IL-17、IL-23、IL-1β、CXCR2 expression at kidney were measured by immunohistochemistry,serum IL-17、IL-23、IL-1β levels were evaluated by ELISA. Result Rats in Crucian carp decoction group had a higher level of albumin compared with Nephrosis group (P=0.03),a lower level compared with Control group(P=0.01) and the expression of IL-17、IL-23、CXCR2 in kidney were lower compared with Nephrosis group (P=0.01，P=0.01，P=0.02)， higher compared with Control group(P=0.04，P=0.007，P=0.05)。Conclusions Increasing Th17 cell inflammatory factors may be the pathogenesis of rats with adriamycin.Crucian carp decoction can improve the albumin level of rats with nephrosis,and alleviate renal impairment induced by IL-17、IL-23and CXCR2.

Abstract:object: To assess the mechanism of building an orthotopic murine bladder tumor model induced by silver nitrate, HCl, trypsin and ethanol. Methods: C57BL/6 mice were anesthetized and catheterized with 24G IV catheter, then divided into 5 groups (each n=6) after washing the bladders with phosphate-buffered saline (PBS); (1) Ethanol: 22% ethanol, incubated for 20min; (2) Trypsin: 0.2%trypsin, incubated for 30 min; (3) HCl: pretreated with 0.1mmol/L HCl for 15 s and 0.1mmol/L NaOH for 5 s after washing the bladders with PBS; (4) Silver nitrate: 0.15mol/L silver nitrate incubated for 10s; (5) Vehicle: normal saline. The mice were killed obtaining the bladder tissue after 1 and 24 hour. Pathological changes were observed by hematoxylin and eosin staining; microenvironment modification were assessed by electron microscope; mast cells were counted with toluidine blue staining; GAG layer were observed by periodic acid-schiff staining. Forty mice were intravesically pretreated with the former four factors, then incubated MB49 cells and determined the rate of tumor “take”. Results: In the model, partial umbrella cells slough, local muscular layer was incontinuous, and submucous layer appeared after 1 hour of pretreatment with trypsin and ethanol. The damage of muscular layer was more serious in HCl and silver nitrate groups. However, there was no statistically significant between experiment and control groups in inflammatory cells. Little edema mucosa and congestion, tight junction, and continuous muscular layer were observed in pretreatment by trypsin and ethanol 24 hours later. By contrast, thickness of muscular layer was inhomogeneity. Conclusion: Pretreatment method of silver nitrate and HCl caused serious damage in muscular layer, and more submucosal collagen and extracellular matrix exposed, compared with trypsin and alcohol. It maybe regard as the optimal choice in establishing orthotopic murine bladder tumor model.

Abstract:Objective To observe the effect of curcumin on the status of cerebral glucose metabolism in 9 months APP /PS1 double transgenic mice by 18F-FDG Micro PET．Methods Nine-month-old APP /PS1 dtgmice were randomly chose from the model group，the positive Rosiglitazone control group and curcumin high(400 mg/kg?d),medium(200 mg/kg?d)and low(100 mg/kg?d)dose group，with normal C57BL /6J mice with the same background randomly selected( age: 9 months; n= 3) for Micro PET study．Each mouse was anesthetized using 2% isoflurane and was injected through a tail vein with 14.8-16.5 MBq 18F-FDG as a bolus． After 1 hour of 18F-FDG uptake period，PET scans were performed for 10-min． Calculate and compare 18F-FDG uptake rate per gram tissue of the whole brain ( except cerebellum) in the mice of different groups． Results Cerebral 18F-FDG uptake rate per gram tissue of curcumin medium dose group mice and the positive Rosiglitazone control group is higher than both the normal control group and model group mice．Conclusions Curcumin can increase APP /PS1 dtgmice cerebral 18F-FDG uptake and 18F-FDG uptake rate per gram tissue． Curcumin may play a neuroprotective role through improving cerebral glucose metabolism.

Abstract:【Objective】 The aim of this study was to assess changes occurring with plasma metabolite parameters and peripheral blood leukocytes（PBL）mRNA expression profiles of genes, mainly involved with energy homoestasis, such as insulin signaling, adiponectin signaling and lipogenesis. Circulating PBL gene expression can therefore potentially provide early warning of any abnormalities present in tissue they discover. 【Method】Using 59 client-owned dogs of differing breeds, which presented themselves at veterinary clinics for routine checkups, body condition score (BCS) of each dog was assessed using a five point scale (lean: 26; overweight: 28; obesity: 5). The following plasma metabolites were measured: glucose; Blood Urea Nitrogen（BUN）; creatinine（CRE）; total cholesterol（T-Cho）; total protein（TP）; triglyceride（TG）; lactate dehydrogenase（LDH）; alkaline phosphatase（ALP）; aspartate aminotransferase（AST）; alanine aminotransferase（ALT）; Non-esterified fatty acid（NEFA）; adiponectin and immunoreactive insulin（IRI）. In addition, RT-PCR was performed to determine PBL mRNA expression of the following genes: 5-Adenosine monophosphate-activated protein kinase [AMPK] -?1 and 2, -?1 and 2, and, -?1 and 2; glucose-6-phosphate dehydrogenase [G6PDH]; malate dehydrogenase [MDH]), fatty acid synthase [FAS]; adiponectin receptor [ADIPOR] (-1 and 2); insulin receptor substrates [IRS] (-1 and 2); phosphatidylinositol-3 kinase [PI3-K]. 【Result】 As compared to lean dogs, overweight and obese dogs demonstrated a significant increase in plasma insulin levels. Moreover, obese status resulted in a significant increase in NEFA, TG and T-Cho. In addition, obese dog PBL demonstrated a reduced mRNA expression profile for IRS-2; FAS; MDH; AMPK α1、β1、β2 and γ2 subtypes genes. 【Conclusion】 These findings suggest that hyperinsulinemia and dysregulation of lipid metabolic, when associated with obesity, in overweight dogs may result in insulin resistance and alterations in PBL gene expression of genes involved in energy homeostatis and sterol metabolism.

Abstract:Abstract: [Objective]Oct4 gene expression research during the developmental stages of porcine parthenogenetic and IVF preimplantation embryos, which is important for embryonic development. [Method]Mature oocytes, parthenogenetic embryos and IVF embryos were collected as follows: 2-cell stage, 4-cell stage, 8-cell stage and blastocyst stage. We used quantitative, real time PCR to assess the relative amount of each transcript in cleavage stage embryos. [Result] Oct4 transcript levels are highest at 8 cell stage (p <0.05), parthenogenetic and IVF blastocysts are lowest expression level when compared to 2-cell stage, 4-cell stage (p <0.05). Parthenogenetic and IVF embryos Oct4 expression has no difference at the same stage.[Conclusion] Transcript levels of pluripotency gene Oct4 change over the course of cleavage development, parthenogenetic embryos to some extent can be used as a model for in vitro embryo gene expression, and different embryo culture conditions may result in a different gene expression.

Abstract:Objective This paper investigated the effects of the moderate-intensity treadmill exercise on microarchitecture of trabecular bone in ovariectomized (OVX) rats by micro-CT. Methods Thirty female SD rats were randomly divided into following three groups: sham-operation (Sham), ovariectomized (OVX), and ovariectomized exercised (EX, 18 m/minute, 45 min/day, 5 uphill, 4 times/week). At 14wk of the regular exercise treatment, the lumbar vertebrae BMD were detected by DEXA. Micro-CT scanning and three-dimensional structure reconstruction were used to analyze the microarchitecture of the third lumbar vertebrae and bonebiomechanical test was carried on the fourth lumbar vertebrae. Results Compared with the OVX group, the BMD of second lumbar vertebrae, the maximum load, maximum stress, and elastic modulus in third lumbar vertebrae, as well as the volume and number of trabecular bone in fourth lumbar vertebrae were significantly higher in EX group whereas the space of trabecular bone was significantly lower. There were no significant differences in the thickness of trabecular bone. Conclusion The moderate-intensity treadmill exercise could benefit the microarchitecture of lumbar vertebra in OVX rats.

Abstract:Objective Mice model by transverse aortic constriction (TAC) was commonly used in cardiomyopathy studies. But the evaluation of successful ligation is a problem. In this paper, we established the TAC mice model consulting references about this technique at home and abroad, and use Doppler as a early stage evaluation technology. Methods The 0.4 mm reconstructed aortic was established theoretically, and the flow of aorta and branches were analysed bydoppler analysis. The mortality of TAC mice was calculated and analyzed by the Spss 16.0 software. The dependablity between doppler analysis on flow 1 week after reconstruction and echocardiography analysis on left ventricle (LV) wall thickness 4 weeks after reconstruction was essaied. This technology was evaluated on references at home and abroad. Reasults The survival rate was 85% (n=40) 8 weeks after reconstriction, while it was 96.4% in the sham group (n=28). The LV posterior wall at end-diastole (LVPWD) will give significance when the IA/LCCA flow ratio>5.9. The IA/LCCA flow ratio will give liner correlation to LVPWD when it is between 5.9 and 10.7, and the liner correlation will decreased when the ratio>10.7. Conclusions The LVPWD will give significant thickness 4 weeks after reconstruction when the IA/LCCA flow ratio>5.9 1 week after reconstruction, and the Doppler analysis can be used as early stage evaluation technology on transverse aortic constriction mice model.

Abstract:Objective: To observation the dynamic circadian fluctuations of the blood pressure in unbonded spontaneously hypertensive rats using Telemetry technology. Method: Taking eight 3-month-old SPF SHR rats, there were implanted C50-PXT device intraperitoneally. Seven days later, monitoring chainless blood pressure for 24 h continuously time using telemetry system. Analysing 24 h ambulatory blood pressure and blood pressure trend diagram used by EMKA analysis software. Result: Blood pressure and heart rate show the circadian rhythm changes in SHR rats of three months, moreover daytime is obvious lower nighttime(P<0.01).Blood pressure appear two periods of peaks at AM1:30-2:30 and PM8:30-9:30,appear low ebb at PM2:00-2:30.Thereinto average of systolic blood pressure is 166.02mmHg in nighttime,two peak of systolic blood pressure was 172.13mmHg and 171.38mmHg respectively. average of SBP is 162.73mmHg in daytime, valley bottom of SBP was 155.73 mmHg.But heart rates appear two periods of peaks at AM1:30-2:00 and PM8:00-9:00,peak value of heart rates were 375.00time/min and 373.26time/min respectively. Valley bottom of heart rates appear at AM 11 or so, bottom peak value of heart rates was 310.91time/min. Average of heart rates were 328.85 time/min and 346.05 time/min at the daytime and nighttime respectively. Conclusion: Blood pressure and heart rate show the circadian rhythm changes in SHR rats of three months. Blood pressure and heart rates appeared two peaks in the night，but was a low point at daytime.The average blood pressure and heart rate of daytime are lower than nighttime. Circadian Rhythm of blood pressure and heart rate was related with activities in SHR rats. Telemetry can accurately reflect blood pressure circadian rhythmicity in SHR rats, contribute to evaluation of antihypertensive drugs and the physiological mechanism of high blood pressure research correctly.

Abstract:【Abstract】 Objective Accumulating evidences have demonstrated that myeloid-derived suppressor cells (MDSCs) play a pivotal role in tumor associated immune suppression. The main purpose of this study was to compare different antibodies to isolate MDSCs form bone marrow of orthotopic liver tumour mice by flow cytometry. Methods The in situ hepatic tumour model was created by the direct intrahepatic injection of mouse hepatoma cells lines H22 (1×106 cells originating from the BALB/c mice). Then the mice were sacrificed in 10 days for anatomy. The monouclear cells were isolated from bone marrow and stained with CD11b,Gr-1 or double stained with CD11b and Gr-1 and then sorted by flow cytometry. The expression of arginase-1(Arg-1) and inducible nitricoxide synthase (iNOS)of isolated MDSCs were detected by flow cytometry. Cellular reactive oxygen species(ROS) detection assay kit was used to measure ROS production by MDSCs. Results The purity and viability of isolated MDSCs by different antibodies was all greater than 90％. Enrichment of MDSCs by CD11b antibody is much more easy, but relative low purity. MDSCs sorted by Gr-1 antibody have higher purity, but the cell population is hard to definite. MDSCs isolated by double staining have the highest purity. MDSCs express high levels of Arg-1, iNOS and ROS. Conclusion High purity and viability of MDSCs can be sorted from bone marrow of hepatic tumour bering mice by different labelling methods.Enrichment of MDSCs by CD11b is easy to operate, unified and copy, which can be served as the first choice in sorting MDSCs . The successful sorting of MDSCs was important for the further studing the biological and immunological function of MDSCs.

Abstract:Abstract：Objectives: To establish a simple acute cerebral ischemia animal model. Methods: 40 male Wistar rats, weighing 200- 230grams, all rats fasting before operation12 hours, free drinking water, were randomly divided into control group, A, B and C group, D group and model group,4,10 each. A groups: sham operation group, only cut on both sides of the neck skin, separation of bilateral internal carotid arteries and vagus nerve, not cut off, and then stitched; group B: only the section of bilateral cervical vagus nerve; group C: only ligated and transected bilateral common carotid artery (CCA ); group D: combined group, namely the ligated and transected cervical total arterial ( CCA ), at the same time section of bilateral cervical vagotomy. Rats were observed after the operation of cerebral ischemic symptoms, rats were recorded within 8 hours of death, more than 8 hours of the death of the animal in8hours, calculation of mortality and death time. Results: A rats had no symptoms of cerebral ischemia, no death; group B rats without symptoms of cerebral ischemia, slow breathing becomes deep, heart rate and blood pressure to rise, but no deaths; the rats in group C part of cerebral ischemic symptoms, ptosis, activity ability is low, a reduction in spontaneous locomotion, but also some rat postoperative spontaneous exercise increases, in 8 h in no deaths; the rats in group D most appeared more obvious symptoms of cerebral ischemia, in full within 8 hours of death. Conclusion: Take the ligation of the bilateral common carotid artery of rats with bilateral cervical vagotomy and cut method, can establish a rat model of acute focal cerebral ischemia animal model, this method has the advantages of simple operation, high success rate, this method has simple operation, high success rate, the slow death of the characteristics of postoperative animals.

Abstract:Objedtive. To establish an acute lethal liver injury mouse model induced by LPS/D-GalN with NF-κB transgenic mouse. Method. To establish an acute lethal liver injury mouse model by i.p. injection of LPS/D-GalN, then to observe the change of serum inflammatory cytokines and activity of NF-κB. Also, function and pathologic changes of liver were checked. Result The acute lethal liver injury mouse induced by LPS/D-GalN lives for 8-10h, and serum levels of TNF-α, IL-6 and MCP-1 increased significantly and peaked in 2-4 hours. Gross examination of the liver of acute lethal liver injury mouse showed a marked congestion and hemorrhage, Also, the liver sections stained by H&E showed that LPS/D-GalN-induced necrosis of hepatocytes, in which the structure of the liver lobules was destroyed and serious necrosis of hepatocytes and hemorrhage occurred. The levels of serum ALT and AST continued rapid rise in acute lethal liver injury mouse. On the whole, NF-kB activities significantly increased, which peaked in 4-6 hours after challenged with LPS/D-GalN. There no such changes in normal control. Conclusion LPS/D-GalN induced acute lethal liver injury model successfully establishedin NF-κB transgenic mice.

Abstract:【Abstract】 objective Study the establishment of newborn Balb / c mouse biliary obstruction model, and sthe model was compared with reported neonatal Balb / c mice infected rhesus monkey rotavirus (RRV). Methods Postnatal 5 to 7 days Balb / c mice were randomly divided into experimental group and the control group, in the experimental group the common bile duct ligation was taken, and then the abdomen was closed. In the control group the abdomen was opened without the ligation of the common bile duct and then was closed. After the completion of the experiment the body weight of the mice, hairless skin color changes, the mice survival days were Observed daily .the murine liver pathology and immunohistochemistry were taken at 5, 10 days . Results Experimental results show that mice after ligation with the time, the body weight of mice, no skin color of the gross area, the number of days of survival, liver pathology and so exist a certain change。The mice weight gain gradually slow and after two days the no gross area of skin turn yellow , yellow liquid exist in the urethra and then Clay stools. Postoperative 5 days and 10 days there is a statistically significant difference (p ≤ 0.05 ) about hepar somatic indices, Mice death peak occurs around 10 days after operation . Conclusion The establishment of Newborn Balb / c mice common bile duct ligation is a reliable model model of study biliary obstruction . Its survival curves is broadly similar to the reported RRV induced biliary atresia.

Abstract:Campylobacter jejuni is a global concerned zoonosis pathogens. It can cause human and animals a variety of diseases if being infected. Animal models are the foundation of doing research on pathogenesis, vaccine evaluation and drug development. Considering the feasibility, economy and repeatability of animal models fastidious bacteria infected, Campylobacter jejuni has no appropriate animal models and its pathogenesis is unclear. This article will describe the experimental animal models for Campylobacter jejuni infections.

Abstract:Objective To study the effects of the mice UNCV protein on Hela cells apoptosis. Methods The recombinant plasmids containing Uncv gene of BALB/C mice were transfected into Hela cells. The Hela cells which stably overexpressed UNCV protein were selected. The effects of overexpressed UNCV protein on apoptosis of Hela cells induced by doxorubicin and serum starvation were measured by cell counting and flow cytometry. Results The Hela cells which stably overexpressed UNCV protein were obtained. The results of cell counting and flow cytometry showed that overexpression of the UNCV protein inhibited apoptosis of Hela cells induced by serum starvation. The effects of UNCV protein on apoptosis of Hela cells induced by doxorubicin were not significantly different. Conclusions Overexpression of UNCV protein inhibited apoptosis of Hela cells induced by serum starvation.

Abstract:To study the mechanisms of hemeoxygenase-1 (HO-1) in ischemic postconditioning attenuating lung ischemia reperfusion injury and its effects on STAT-3 in postischemic lung of rats. Methods: Forty male SD rats were randomly assigned to shame group (S), ischemia-reperfusion injury group (IR), ischemic postconditioning group (IPO) and IPO with HO-1 inhibitor ZnPP treatment group (IPO ZnPP). The ratio of dry / wet weight (W/D), the level of MDA, and the activity of MPO and HO-1 were measured in postischemic lung tissures. The protein expression of HO-1 and p-STAT-3 were detected by Western blot. Results: The levels of W/D, MDA, MPO, HO-1 activity and its protein expression were all significantly increased as compared with the S group, while the expression level of p-STAT-3 protein was significantly decreased, IPO can reverse these changes, and the specific inhibitor of HO-1 Znpp canceled the effects of IPO on the above indexes. Conclusion: IPO attenuates lung ischemia-reperfusion injury by promoting HO-1 induced activation of STAT-3 signaling pathway.