Abstract:Objective To establish a SKH-1 hairless mouse model of skin and lung infection by methicillin-resistant Staphylococcus aureus(MRSA) aerosol and its evaluation system. Methods 48 SKH-1 hairless mice were randomly divided into PBS control group, infection group and infection with scald group. After the dorsal skin of the infection with scald group were thermally injured, mice were challenged with aerosolized MRSA(ST-239) through the respiratory tract, then the models were evaluated by body weight, blood tests, bacteraemia rate, skin and organs infection measuring, histopathological observations and multi-PCR for the nuc and mecA genes detection. Results Compared with the other two groups, the infection with scald group had evident weight loss, remarkable increase of WBC count and a bacteraemia rate of 100%. The number of viable S. aureus in the skin and lungs was 108CFU/g and 106CFU/g respectively, inflammatory lesions of the infected tissues were obvious under the light microscope. The results of immunohistochemistry showed that CD138 expressed positively in the skin, and positive results were also found with both nuc and mecA genes in the infected tissues of the infection with scald group. Conclusion We established a scald SKH-1 hairless mouse model, challenged with aerosolized MRSA, which can well simulate clinical burn patients with secondary infection of MRSA pneumonia. This mouse model is of important clinical application value for the evaluation of MRSA-related antimicrobial treatments.

Abstract:Objective To isolate and identify Murine Norovirus Virus (MNV) from the infected mice breeding in four facilities in Guangdong Province. To illuminate molecular genetic features and evolutionary origin of MNV isolates by analysing nucleotide sequence of MNV capsid protein (VP1) gene. Methods RAW264.7 cell line was used to virus isolation from the cecal contents of the infected mice tested by RT-PCR. Cytopathic effect (CPE) assay, RT-PCR technology, indirect immunofluorescence assay and nucleotide sequencing was used to identified the MNV isolates. Fragments of 1626 nucleotides including VP1 genes from 15 MNV isolates were amplified by RT-PCR, the PCR products were ligated into pMD18-T vector and cloned to DH5α cell. By ampicillin plate selection, the positive clones were sequenced and analyzed. Based on the 1626 nucleotides of VP1 gene, the phylogenetic analyses were processed with 19 MNV strains down loaded from GenBank and 15 MNV isolates from Guangdong Province. Results 15 MNV isolates were isolated from 80 clinical samples. Cytopathic effect (CPE) was observed by microscopy, the isolates was identified as MNV by RT-PCR technology, indirect immunofluorescence assay and nucleotide sequencing. The length of the VP1 gene was 1628 nucleotides. 15 MNV isolates showed a nucleotide and amino acid similarity of 89.7~100% and 94.8~100% respectively. The homology of nucleotide and amino acid between 15 isolates and other MNV isolates which were registed in GenBank database were 87.5~92.9% and 92.4~98.2% respectively. Phylogenetic analysis showed that there was a close genetic relationship between strains isolated from mice of Facility A and Facility D, all the 13 isolates belonged to a the same evolutional branch. The strains ZD-1（isolated from mice of from Facility B ）and strains ZYY-163（isolated from mice of from Facility C）belonged to an another evolutional branch, this branch also included K162, S7-P2, S7-PP3, K4, Berlin/04/06/DE and Berlin/05/06/DE. Conclusion 15 MNV isolates were isolated and identified successfully. Evolutionary origin of 15 MNV isolates from Guangdong region were different. Two MNV isolates from Facility B and Facility C showed a close genetic relationship with the foreign MNV isolates, but 13 MNV isolates from Facility A and Facility D are likely to be the local strains already existing in Guangdong Province.

Abstract:Objective Through the preliminary study of immunology on rabbit model of allergic purpura, it’s hopeful to explore the immunological mechanism of the disease. Methods Blood routine to detect immune cells; enzyme-linked immunosorbent assay (ELISA ) to measure the content of lymphocytes and cytokines CD3, CD4, CD8, CD4/CD8, IL-2, TNF-α; immunofluorescence assay for detecting IgA, IgG and C3 in skin and kidney; special dyeing included masson staining and PAS staining of the renal glomerulus, Luna dyeing of skin and lung tissue. Meanwhile, this will be compared with the control group for analysis. Results Compared with the control group, the rabbits of model group showed the increased number of white blood cells (WBC), neutrophil (NEU) and the percentage, eosinophil(EOS) and the percentage, basophilic granulocyte(BAS), and the difference had statistical significance; the content of peripheral blood CD3+T, CD4+T lymphocytes decreased, CD8+T lymphocytes increased, the ratio of CD4/CD8 dropped, the level of IL-2 decreased and TNF-α increased, the difference of all had statistical significance; IgA, IgG, C3 expression in skin and kidney increased; glomerular performed that collagen fibers proliferation, mesangial thickening, mesangial matrix increased; the numbers of eosinophils in dermis and lung tissue had more positive expression. Conclusion Through the study of the immune cells and cytokines, we found that immune function disorder existed in the allergic purpura rabbit model. These results will provide valuable basis for clarifying pathogenesis of HSP, finding out the new indicators for clinical diagnosis, curative effect observation and selecting the appropriate treatment schedule.

Abstract:【Abstract】 Objective: Explore the method and evaluation of establishment of the stress-induced rats of intestinal dysfunction. Methods: Sprague-Dawley rats were assigned to two groups randomly: the normal group and the model group . The model group was treated by using chronic astricting, excess fatigue and out of constant diet .It would continue for three weeks. At the last day of the third week, the content of GAS, IL-2, in serum, MTL, SS, ACTH, in plasma were measured by the method of ELISA kit. Results: Compared with the normal group, the model group had many different and apparent changes of appearance and action, their weight and the mass of their livers and spleens are much less than the normal group, the level of these biomarkers are reducing apparently. Conclusion: Stress-induced rats of intestinal dysfunction can be successfully established by using chronic astricting, excess fatigue and out of constant diet in the SD rats, and the biomarkers such as GAS,IL-2,ACTH,MTL,SS can be used to evaluation of the model.

Abstract:【Abstract】 Objective To explore the effect of gain-of-function mutation SHP-2D61G/+ and SHP-2D61G/D61G on adhesion, migration and proliferation of mice MEFs cell, and to study the expression levels of p-ERK in gain-of-function mutation SHP-2 of MEFs cells. Methods The mice were mated to establish SHP-2D61G/+ 、SHP-2D61G/D61G gain-of-function mutation MEFs, and were immortalized by SV40T antigen. The effect of Gain-of-Function Mutation SHP-2 D61G/+ 、SHP-2D61G/D61G on the adhesion ability of MEFs cell was detected by cell adhesion assay. The transwell migration in vitro was applied to detect the effect of Gain-of-Function Mutation SHP-2 D61G/+ 、SHP-2D61G/D61G on migration ability of MEFs cell. The effect of gain-of-function mutation SHP-2 D61G/+ 、SHP-2D61G/ D61G on proliferation ability of MEFs cell was tested by MTT, and Western Blot was used to check the p-ERK expression. Results (1) Compared with control group, SHP-2D61G/+ 、SHP-2D61G/D61G gain-of-function mutation of MEFs cell significantly increased in the number of adherent cells, the difference had statistically significant. (2) Compared with control group, SHP-2D61G/+、SHP-2D61G/D61G gain-of-function mutation of MEFs cell significantly increased in the number of migration cells, there was significant difference. (3) The results of MTT showed that the proliferative capacity of the MEFs cell after SHP-2D61G/+ 、SHP-2D61G/D61G activating mutations were stronger than the control group, the difference had statistically significant. (4) Western Blot showed that compared with control group , whether just adherent or adherent after 30min and 60min, the p-ERK expression level of SHP-2D61G/+、SHP-2D61G/D61G were all increased. Conclusion The adhesion, migration and proliferation ability of mouse MEFs cell can be promoted by SHP-2 D61G/+ 、SHP-2D61G/D61G gain-of-function mutation. The level of p-ERK was increased in SHP-2 activating mutations MEFs cells.

Abstract:Objective To study the anticancer effects and efficacy enhancing and toxicity reducing of Ginseng polysaccharide (GPS) on mice who are treated with chemical therapeutic drug like cytoxan(CTX). Methods KM mice were used to established mice liver H22 tumor model, and randomly divided into negative control group, GPS group, CTX group, compounds of GPS and CTX group. Administration of GPS and CTX was by intravenous injection and intraperitoneal injection respectively. After 10d consecutive administration, measuring its tumor inhibitory rate, Hematological indexes, spleen index and thymus index. Results The tumor inhibition rate was higher in each GPS , GPS CTX group than that in control group ( P<0. 01)． The tumor inhibition rate of medium-dose and high-dose GPS CTX group was higher than that in CTX group (P < 0. 05, P < 0. 01) ．The indexes of spleen and thymus as well as body weight in CTX group were lower than those in the high-dose GPS CTX group (P<0.05，P<0.05，P<0.05). Conclusion GPS can enhance the efficacy of chemotherapy and reduce its toxicity in the H22-burdened mice exposed to CTX．

Abstract:This research builds the improved method of atherosclerosis rats mold based on the literature, which aims to establish a suitable coronary heart disease wistar rats model for cardiovascular research. To overcome the wistar rats’s resistance to cholesterol induce atherosclerosis, animals were given D3 vitamin at 150000 IU :rat per month associated with cholesterol-enriched diet for first 3 months, following 2 months of high cholesterol diet alone. Blood serum parameters (at days 0,30,90,120 and 150) and coronary artery, aortic and heart morphology (at days 120 and 150)were examined. Other animals receiving a normal diet were used as a control group. Results showed that at day 90 severe hypercholesterolemia, elevated blood serum LDL-C and CHO, oxidized LDL, AngⅡ,sICAM-1.Lesions were characterized by widening of the first interlamellar spaces in the aorta, fibrosis of coronary arterial arterial wall and recent foci of myocardial fibrosis. At day 150d oxidized LDL, AngⅡ,sICAM-1 and ET-1were more enhanced. The D3 vitamin administration induced advanced atherosclerotic lesions in arterial wall, represented by the rupture of elastic lamellae, smooth muscle cell proliferation and lipid-calcic core. The complicated plaque frequently evolved into ulcerations. The ischaemic effects were represented by acute myocardial infarction. D3 vitamin is an atherogenic agent which, when associated with hypercholesterolemia, allows the development of advanced atherosclerotic lesions in wistar rat which resembles human plaque.

Abstract:Abstract : Muscle mass and strength are well known to decline in response to actual and simulated microgravity exposure. Until now, the mechanism of muscle atrophy under microgravity is still unclear and there is no specific medical intervention. The review examines the effects and mechanisms of actual and simulated microgravity on muscle wet weight, muscle fiber cross-sectional area, muscle fiber type, muscle ultrastructural changes, and adaptive changes in muscle spindle, which is to summarize the major effects of space travel or simulated microgravity on skeletal muscle atrophy.

Abstract:Objective, This paper introduces the working principle and the method of animal lung function measure system, and establishes lung function normal reference values of the Wistar rat. Methods ,Small animals lung function detector is utilized to detect the normal lung function index in rats , According to the test results of lung function index, through statistical analysis, wen can determine the normal reference range of the Wistar rat.Results, The normal range of the inspiratory resistance (Ri) 、the expiratory resistance (Re)、the compliance (Cl)、the maximal voluntary ventilation (MVV)、the forced vital capacity (FVC)、the ratio of forced expiratory volume at 0.2 second (Fev0.2)、the ratio of forced expiratory volume at 0.2 second and forced vital capacity (Fev0.2/FVC)、the forced expiratory flow (PEF25-75%)、the peak expiratory flow (PEF) is 1.81(0.94～4.10)cmH20/mL•s、1.83(0.71～3.57)cmH20/mL•s、0.15（0.05～0.29）mL/cmH20、144.65 (77.28～256.20)mL/min、8.49(5.82～12.70)mL、5.72(3.62～7.01)mL、68.12(39.14～85.28) %、34.11(28.25～46.87)mL/min、38.32（30.86～50.28）mL/min。 Conclusions, Researching and determineing the normal reference rang of lung function of Wistar rats,could provide reference basis for clinical and scientific research work and establishment of national standards and specifications in the future.

Abstract:Cerebral ischemia has become a research focus in recent years. Within various animal models, gerbil has becoming the ideal cerebral-ischemia model because of its incomplete basilar artery rings. Gerbil model has many advantages in both unilateral and bilateral cerebral ischemia research. So it has a wide application to the research of cerebral pathological after ischemia, the injury mechanism of reperfusion, the innovation of anti-cerebral ischemia new drugs. The applications of animal model especially the gerbil model in the field of cerebral ischemia were reviewed in this paper.

Abstract:Objective To understand various behaviors of Microtus fortis under different environment lighting stimulation and collect the basic data for formulating the environmental control standard in breeding Methods Three groups of Microtus fortis were observed using the special apparatus for animal behavior observation under different environment light stimulation. Results The 24D group was at most in locomotor activity and the 24L group was at least. The 12L/12D group was at near the 24D group. No obvious and clear rhythmicity of locomotor, whether in group 24L or in group 24D,was found during lighting treatment; but under 12lL/12D condition, the locomoter appeared quite regular and increased with the time of lighting, The 12L/12D group was several times as much as in the other two groups in play behavior and the 24D group was more than the 24L group . The vole was very few in the center under condition of 24L. but animals were shuttled to the central in 24D and 12L/12D lingting. The 12L/12D group was much more at feeding and drinking while the 24L group was very little. Conclusion Indoor regular environmental light treatment, the behavior of Microtus fortis has inherence in photoperiod response. The 12L/12D group is the most suitable light environment for Microtus fortis. 〔Key words 〕Microtus fortis；environment lighting；locomotor；rhythmicity；suitable environment

Abstract:With the deepening of miniature pigs research, as the advantages of human disease model has become increasingly evident, the application in the biomedical research is also on the rise. Comparative anatomy and physiology studies have shown that many biological characteristics of miniature pigs are very similar to humans, especially in the anatomy and function of the kidneys is almost a replica of the human, make it in duplicate kidney disease model, research the pathogenesis of the disease and evaluate treatment strategies has an irreplaceable role. In this paper, we will review miniature pigs as the disease animal model application in kidney disease research.

Abstract:Objective: To investigate the methods and experimental conditions of constructing myocardial endoplasmic reticulum stress (ERS) model in vitro. Methods: Langendorrf perfusion device was used to make rat heart ischemia/reperfusion model in vitro, and left ventricular end-systolic pressure (LVESP), left ventricular end-diastolic pressure (LVEDP), heart rate (HR) and the maximum rate of change of left ventricular pressure (+/-LVdp/dtmax) were continuously monitored by the PowerLab system. The expression of ERS classical marker glucose-regulated protein (GRP) 78 in myocardium was detected by western-blot analysis after ischemia (stop perfusion) different times (30min, 35min, 40min and 45min) / reperfusion 120min, the expression of C/EBP homologous protein(CHOP) was detected, too, and the mRNA level of GRP 78 and CHOP were detected by reverse transcription-polymerase chain reaction（RT-PCR）; myocardial tissue slices were incubated in vitro and treated by ERS stimulant tunicamycin (Tm) or dithiothreitol (DTT) at different concentrations for 3h and 6h, respectively, and the expression of GRP78 and CHOP were detected by western-blot. Results: The highest expression of GRP78 protein was detected in myocardium from the isolated perfused rat heart with ischemia 40min / reperfusion 120 min(P <0.01), CHOP protein、GRP78 mRNA and CHOP mRNA increased significantly（P <0.01，P <0.05和P <0.05） and at the same time, compared with control group, HR, ±LVdp/dtmax, coronary perfusion flow(CPF), and left ventricular pressure(△LVP, the difference between LVESP and LVEDP) decreased, and LVEDP increased (all P <0.01) . Compared with control group, myocardial tissue slices incubated with Tm / DTT for 3h, GRP78 in Tm10μg/mL group and DTT 2 mmol/L group significantly increased (both P <0.001); GRP78 in DTT 10 mmol/L group increased (P <0.05), but not as much as that in DTT 2 mmol/L. The expression of CHOP increased significanly, too（P <0.05 and P <0.01）. Conclusion: Using both isolated rat heart ischemia/reperfusion and myocardial tissue slices incubation methods, the in vitro myocardial ERS model can be successfully constructed.

Abstract:Objective :To observe the effects of rehabilitative training on cAMP and PKA expression, motor function in cerebral ischemia-reperfusion rats, for explore its mechanisms. Methods:The middle cerebral artery ischemia-reperfusion model was established by suture-embolus method in rats. 42 rats were randomly assigned to ischemia group, rehabilitative group and group. The changes of cAMP and PKA expression were determined by enzyme-linked immunosorbent assay (ELISA). Behavioral recovery was quantitated by scores. Results :（1）The expression of cAMP and PKA showed significant higher in rehabilitative group than the ischemia group.（2）The motor functional recovery of rehabilitative group was significantly better than the ischemia group . Conclusion: Rehabilitative training promote motor function recovery in cerebral ischemia-reperfusion rats that it was concerned with cAMP-protein kinase A signal pathway.

Abstract:Objective To establish a mouse model of type II diabetes mellitus complicated by tuberculosis and make a evaluation to the process of lesions in lung tissue. Methods Feed the mice with high-fat and high-sugar, inject intraperitoneally STZ to C57BL/6J mice, create the type II diabetes mellitus mouse model. Then, infected with Mycobacterium tuberculosis H37Rv by nasal drip, to establish a mouse model of type II diabetes mellitus complicated by tuberculosis. Dissect the mice separately at 1-8th, 10th and 12th week after infection, make visual observation to the lesions of lung, colony counting and pathological examination, HE and acid-fast staining. Results The visual observation results showed that the lung infection has deteriorated as the infection time increased. The focus of the infection reached to 80% of the whole lung at the 8th week, and the scope of lesions began to shrink at the 10th week. The typical granulomatous consolidation appeared in lung tissue after 8 weeks of the infection, and till the 10th week, the part of the lesions of lung tissue began to repair. The mycobacterium tuberculosis in the lungs were revealed by acid-fast stain. Conclusions Compare with the tuberculosis mouse model in gross inspection, colony counting, pathological changes, the type II diabetes mellitus complicated by tuberculosis mouse model is characterized with early onset , fast disease progression, and similar to the patients with clinical performance. Therefore, the experiment proves that the establishment of the type II diabetes mellitus complicated by tuberculosis mouse model is successful.

Abstract:As the world population continues to age, Alzheimer's disease has become one of the major diseases that threaten the health of the elderly. To research and establish a reliable animal model for Alzheimer's disease is very important to clarify the disease etiology, pathogenesis and develop the prevention drugs. This article reviews the most widely used and the most in-depth studied transgenic mouse models with their characteristics of pathology and behavior changes、with their application and discoveries in Alzheimer's disease research currently.

Abstract:To determine the transfection of tyrosine phosphatase SHP2（Src homology phosphyrosyl phosphatase 2） eukaryotic expression (pcDNA3.1SHP2) vectors in mouse Lewis lung carcinoma (LLC) cells by ultrasound mediating and to observe SHP2 overexpression on Lewis lung carcinoma (LLC) cell proliferation and invasion and molecular mechanism of influence. Methods After addition of pcDNA3.1SHP2 vectors and 5μL LipofectamineTM 2000, mice Lewis lung carcinoma cells (LLC) is added to each well of 6-well plates were explored to diagnose ultrasound irradiation dose 60s in the presence(200μL) ultrasound agent. Expression of SHP2 and P38 protein was determined y by Western blotting. MTT, scratch test and Transwell system were used to detect the proliferation, migration and invasion of LLC cells, after 48h. Results Overexpression of SHP2 gene in LLC cells could be detected and phosphorylation p38 up-expression by Western blotting. Overexpression of SHP2 increased significantly cell proliferation, migration and invasion. Conclusion Overexpression SHP2 enhances tumor cell proliferation, migration and invasion, and preliminarily confirmed SHP2 overexpression may activate mitogen-activated protein (MAPK) signaling pathway promotes malignant progression of tumor cells.

Abstract:【Abstract】 Objective Carrying out the preliminary research on the value of Biotechnology Fulvic Acid (BFA) as nutritional additives in functional feed, based on the biochemistry and intestinal flora index of mammals(SD rats) affected by BFA. Methods Feed four group of mammals (SD rats) with normal feedingstuff mixed of four kind of BFA(lactobacillus fermented BFA, bacillus fermented BFA, yeast fermented BFA and Mixed fermentation BFA) with the proportion of 1.5%, compare with the group of mammals (SD rats) feeding without BFA, observe the indicator of nutrition and health condition, including weight gain, feed weight ratio, routine blood test, blood biochemical indices and quantitative analysis of intestinal flora. Results 1. Routine blood test: WBC, MON, NEU decreased, the difference is significant(P<0.05,P<0.01);NEU of bacillus fermented BFA group decreased，the difference is significant(P<0.05). 2.Blood biochemical indices: TP, Alb, A/G of lactobacillus fermented BFA group increased, the difference is significant(P<0.05,P<0.01,P<0.05),TBIL decreased, the difference is significant(P<0.05);TBIL of bacillus fermented BFA group decreased, the difference is significant(P<0.01). 3.weight gain: the Total Weight Gain and the Daily Weight Gain of Mixed fermentation BFA group is evidently higher (P<0.05);Weight ratio of lactobacillus fermented BFA group, bacillus fermented BFA group, yeast fermented BFA group, Mixed fermentation BFA group to the normal group is 6.70,6.74,6.49,6.09,6.77. 4.Quantitative analysis of intestinal flora: The number of Escherichia coli of lactobacillus fermented BFA group and Mixed fermentation BFA group decreased，the difference is significant(P<0.05),meanwhile the number of bacillus acidi lactici evidently increased(P<0.05). Conclusion BFA improves the nutriture and health indicator of SD rats. the nutritional effects of Mixed fermentation BFA is the best of all. The experiment proved the mixed fermentation BFA is remarkably valuable for improving health condition of mammals as nutritional additives in functional feed.

Abstract:Neuralepithelial transplantation plays a critical role in axon pathfinding of motor neural and migration of neural crest cells during early development of vertebrate embryo. The animal model of chick embryo and chick-quail chimera was used to clarify a mature method of neuralepithelial transplantation in our study. Many drawbacks such as embryo death, less repeatability and cumbersome processes etc. exist in traditional transplantation. Hence, the advantages of foreign microsurgery transplantation were used as the mirror, as well as our trial-and error in our methods. Embryo pre-treatment, microneedle preparation and some details were present in this paper to highlight how to achieve a successful micro-transplantation. The protocol will dedicate to developmental neurobiology, such as migration of neural stem cells and axon pathfinding research early embryo development.

Abstract:Animal welfare plays a very important role in atrial fibrillation animal model experiment.Laboratory animals suffer pain of physical and mental experience.Animal welfare also influents the outcome data from the experiment.This review describes a a variety of of refinings in before during and after surgery of atrial fibrillation animal model experiment.including the improvement of Pre-operative animal acclimatisation Surgical expertise technique Post-operative animal management and evaluation Anesthesia and pain management.

Abstract:【ABSTRACT】Objective Observation of rotenone Parkinson's disease (PD) rat brain iron accumulation area whether glial cell is activated. Methods Male Wistar rats are daily subcutaneous injection of rotenone sunflower oil emulsion (2mg/kg) in 4 to 6 weeks(once per day) for preparation rotenone rat model of Parkinson's disease, making frozen sections, and immunohistochemical staining, Observation of PD rats brain iron accumulation zone microglia (OX42) and astrocyte (GFAP). Results PD rat's substantia nigra pars compacta(SNC), striatum globus pallidus(SGP), dentate gyrus granular layer of the hippocampus(HDG), dentate-interpositus and the facial nucleus of the cerebellum(CDI,CFN) area of iron staining microglia and astrocyte stained integral optical density values were significantly increased compared with normal group. Conclusion Glial cells are activated in rotenone PD rat brain iron accumulation zone.

Abstract:Objective To study the method of insulin resistance model and effect of 1,1-Diphenylethylene on the regulation of blood sugar. Methods 1. Fat emulsion was given the Wistar rats and made animal models of insulin resistance. 2.Insulin was given HepG2 cells and made HepG2/IR cells model of insulin resistance. 3. Three were given a model in rats and HepG2/IR cells 1,1-Diphenylethylene observed regulation effect of 1,1-Diphenylethylene on blood sugar. Results Level of blood glucose, TG, the TC and LDL HDL of rats after giving the fat emulsion, were increased by 72.87% and 16.21%，139.93%，56.93%，18.32%,compared with the modeling before, the difference was significant (P<0.01); after giving 1,1-Diphenylethylene,levels of blood glucose and lipid significantly reduced than before administration (P<0.05 to 0.01); glucose consumption of HepG2/IR group increased significantly than that of control group(HepG2). Conclusions The insulin resistance models were copied by given to Wistar rats fat emulsion or given to HepG2 cells insulin; 1,1-Diphenylethylene has effects of reduce level of blood glucose and lipids of animal models, increase glucose consumption of HepG2/IR cells