Abstract: Objective　To explore the impact of ten-eleven translocation 2 (TET2) mutations on imiquimod (IMQ)-induced psoriatic skin inflammation using a TET2-knockout (TET2^-/-) mouse model. Methods　Mice were divided randomly into a wild-type (WT) vaseline group, WT imiquimod group, TET2^-/- vaseline group, and TET2^-/- imiquimod group. IMQ was used to establish a psoriasis-like dermatitis model, and the degree of skin lesions and pathological changes in mice in the WT imiquimod and TET2^-/- imiquimod groups were observed and compared daily during the modeling period. The mice were sacrificed when the phenotype had reached the peak and the spleen index was recorded in each group. Gene expression levels of the inflammatory factors tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-17A, and IL-23 in mouse back lesions were detected by quantitative reverse transcription polymerase chain reaction. Skin histopathology was compared in hematoxylin/eosin-stained sections. IL-17, interferon (INF)-γ, and TNF-α protein expression levels in the back skin of mice in the four groups were detected by immunohistochemistry. The ultrastructure of the dermis and epidermis was observed using transmission electron microscopy. Results　TET2 expression was down-regulated in skin lesions in WT imiquimod group. Dermatitis lesions were more severe and progressed faster in TET2^-/- imiquimod group compared with WT imiquimod group, and the psoriasis area and severity index score and spleen index were both higher. mRNA expression levels of TNF-α, IL6, IL-17A, and IL-23 in skin lesions were higher and epidermal thickening and inflammatory cell infiltration were increased, and protein expression levels of IL-17, INF-γ, and TNF-α were significantly higher in skin lesions in TET2^-/- imiquimod group compared with WT imiquimod group. In addition, cell junctions were absent in skin lesions in TET2^-/- imiquimod group and mitochondrial ridges were broken and dissolved, mitochondrial vacuoles were present, and the texture of the mitochondrial membrane was darker. Conclusions　Loss of TET2 promotes the inflammatory response and exacerbates IMQ-induced psoriasis-like dermatitis injury in mice.

Abstract: Objective　To compare the success rate and stability of rat models of comorbid chronic pain and depression induced by different doses of complete Freund’s adjuvant (CFA). Methods　Sixty SD rats were divided randomly into a control group, low-dose CFA group (CFA-L), and high-dose CFA group (CFA-H) (n = 20 rats per group). Rats in the CFA-L and CFA-H groups were injected with 50 and 100 μL CFA, respectively, and rats in the control group were injected with 0.9% sodium chloride solution. The general state, body weight, mechanical withdrawal threshold (MWT), and thermal withdrawal latency (TWL) were observed at 0, 7, 14, 21, and 28 days after modeling. Depressive behavior was evaluated using the open field test (OFT), forced swim test (FST), and tail suspension test (TST). Glutamate (Glu) and γ-aminobutyric acid (GABA) levels in the anterior cingulate cortex were detected by enzyme-linked immunosorbent assay. Brain-derived neurotrophic factor (BDNF) expression in the anterior cingulate cortex was detected by immunohistochemistry, and pathological changes in the anterior cingulate cortex were observed by HE staining. Results　(1)Regarding the general condition of the rats, the left ankle joint and toes were obviously red and swollen in the CFA-L and CFA-H groups on the 7th day after modeling, and the swelling was more severe in the CFA-H group. The redness and swelling of the left hind foot and ankle joint and toes gradually recovered in the CFA-L group on days 14, 21, and 28 after modeling, but were still obvious in the CFA-H group, and the water and food intake decreased. (2)The body mass was significantly lower in rats in the CFA-H group compared with those in the blank and CFA-L groups on days 14, 21, and 28 after modeling (P<0.05, P<0.05). (3)Regarding pain-related behavior, the MWT and TWL were significantly decreased in the CFA-L and CFA-H groups on the 7th and 14th days after modeling, compared with the control group (P<0.05, P<0.05). On day 21 after modeling, MWT was significantly lower in the CFA-H group than in the blank and CFA-L groups (P<0.05, P<0.05), and TWL was significantly lower in the CFA-L and CFA-H groups than in the blank group (P<0.05, P<0.05). On day 28 after modeling, MWT and TWL were significantly lower in the CFA-H group than in the blank and CFA-L groups (P<0.05, P<0.05). (4)In terms of depression-related behaviors, the total OFT movement distance was significantly lower in the CFA-H group than in the blank and CFA-L groups on day 7 after modeling (P<0.05, P<0.05). The total OFT distance and central dwell time were significantly lower in the CFA-H group than in the blank and CFA-L groups on days 14, 21, and 28 after modeling (P<0.05, P<0.05), and the result in the FST and TST were significantly higher than in the blank and CFA-L groups (P<0.05, P<0.05). (5)Glu, GABA, and BDNF expression levels were significantly higher in the CFA-H group than in the blank and CFA-L groups (P<0.05, P<0.05), while GABA, Glu/GABA, and BDNF levels were significantly lower in the CFA-H group than in the blank and CFA-L groups (P<0.05, P<0.05, P<0.05). (6)The CFA-L group showed less damage in the anterior cingulate cortex, more pyramidal cells, more arranged cells, clear nucleoli, and a small number of cells with karyokynesis and deep staining. Compared with the CFA-L group, rats in the CFA-H group showed a disordered cell arrangement in the injured area of the anterior cingulate cortex, a large number of pyknotic and hyperchromatic neurons, significantly fewer or absent pyramidal cells, and vacuoles, red blood cells, and neurofibrillary tangles in the interstitial space. Conclusions　Injection of CFA 100 μL can be used to establish a rat model of chronic inflammatory pain and depression, showing hyperalgesia, depression-like behavioral changes, changes in levels of Glu, GABA, and BDNF in the anterior cingulate cortex, and pathological changes in the anterior cingulate cortex, consistent with the pathophysiological characteristics of chronic pain and depression.

Abstract: Objective　Cigarette smoke (CS) exposure combined with Klebsiella pneumoniae (KP) infection in mice was used to establish a model of chronic obstructive pulmonary disease (COPD) to investigate the mechanism of airway remodeling. Methods　Male BALB/c mice were randomly divided into a Control group, CS group, KP group, and CS + KP group. The mice were exposed to CS, KP, and CS + KP from weeks 1 to 8, and were sacrificed in weeks 4, 8, 16, and 24. MV, Penh, MLI, MAN, and changes in lung pathological structure were detected. The expression levels of IL-1β and TNF-α in lung tissue were detected by ELISA. Collagen deposition was observed by Masson staining and immunohistochemistry. α-SMA and TGF-β1 expression in lung tissue was detected by immunofluorescence. Human bronchial epithelioid cells (16HBE) were also stimulated by CS and lipopolysaccharide (LPS) in vitro, and the expression levels of airway epithelial junction proteins, autophagy-related protein, and mTOR signaling proteins were detected. Results　Compared with the Control group, the CS + KP group mice had significantly decreased MV from weeks 4 to 24 (P<0.05 or P<0.01) and significantly increased Penh from weeks 8 to 24 (P<0.05 or P<0.01); while the CS group had markedly decreased MV and markedly increased Penh from weeks 8 to 16 (P<0.05 or P<0.01). Compared with the Control group, massive inflammatory cell infiltration, alveolar wall thickening, alveolar rupture and fusion, and airway wall thickening were observed by HE staining in CS + KP group from weeks 4 to 24. The CS + KP group mice had significantly decreased MAN and significantly increased MLI, IL-1β and TNF-α in their lung tissue from weeks 4 to 24 (P<0.05 or P<0.01). The aforementioned inflammation and tissue damage were observed in the CS group and the KP group from week 8 to 16. Compared with the Control group, COL Ⅰ, COL Ⅲ, α-SMA, and TGF-β1 were significantly increased in lung tissue of mice in the CS + KP group from weeks 8 to 16 (P<0.01); COL Ⅰ was significantly increased in the CS group and KP group from weeks 8 to 16 (P<0.01). In addition, increased E-cad and decreased N-cad (P<0.05); significantly decreased LC3B and Beclin-1 (P<0.05); and significantly increased p-mTORC1, p-P70-S6K, and p-4E-BP1 expression were observed in 16HBE cells exposed to CS and LPS (P<0.05 or P<0.01). Conclusions　Pulmonary functional decline, pathological changes in lung tissue, and airway remodeling appeared to occur early and persist in COPD mice induced by CS and KP. The mechanisms may be related to the activation of mTORC1 signaling pathway and subsequent inhibition of autophagy.

Abstract: Objective　To observe the characteristic symptoms in breast cancer-bearing mice and the beneficial effect of ginger-processed Jiangxiangru polysaccharides on traditional Chinese medical symptoms. Methods Thirty-eight mice were used for modeling and divided into normal, model, positive, and low-, medium-, and highdose ginger-processed Jiangxiangru polysaccharide groups. Mice in the normal group were not inoculated with tumors and mice in the normal and model groups received physiological saline intragastrically. Mice in the positive group received celecoxib solution intragastrically, and mice in the low-, medium-, and high-dose groups received the same dose but different concentrations of ginger-processed Jiangxiangru polysaccharide solution intragastrically. Changes in body weight and tumor size were recorded after 4 weeks of continuous administration. Symptoms were observed at the end of the experiment. RGB values in photographs of the tongues, tails, and claws from mice in each group were analyzed and recorded. The degrees of blood deficiency, yin deficiency, and tumor phlegm stasis were calculated based on the method of quantitative dialectical diagnosis. The tumors were isolated and weighed, and the tumor volume and inhibition rate were calculated to determine the beneficial effect of ginger-processed Jiangxiangru polysaccharides on traditional Chinese medical symptoms. Results　Mice in the breast cancer model group showed signs of blood deficiency, yin deficiency, and phlegm stasis. Tumor size was significantly reduced in mice in the ginger-processed Jiangxiangru polysaccharide groups. Ginger-processed Jiangxiangru polysaccharides inhibited tumor growth and improved blood deficiency, yin deficiency, and tumor phlegm stasis in breast cancer-bearing mice, with the best result in the high-dose group. Conclusions　Ginger-processed Jiangxiangru polysaccharides can improve the symptoms of blood deficiency, yin deficiency, and tumor phlegm stasis in breast cancer-bearing mice, especially at high doses.

Abstract: Objective　To evaluate the feasibility of establishing a portal vein thrombosis (PVT) model in cirrhotic rats. Methods　Fifty male SD rats aged 10 ~ 12 weeks and body mass about 300 ~ 350g were divided randomly into a model group and a blank group. Cirrhosis was initially established in the model group. PVT was then established by intermittent portal vein ligation with clamping, and was confirmed by hepatic color Doppler ultrasonography 1 week after modeling. The model group was then divided randomly into a model control group and a model recovery group. Liver and portal vein tissues were extracted from the model control and blank groups after laparotomy, and from the model recovery group after continued feeding for 2 weeks. Liver and portal vein samples in each group were stained with hematoxylin eosin (HE) and Masson stain and portal vein samples were stained with Elastica van Gieson (EVG) stain. Results　Ultrasound examination showed stable thrombus formation in the portal vein in the model group 1 week after surgery, with a modeling success rate of 68%. HE and Masson staining showed false lobules and PVT, media edema and thickening, and collagen fiber adhesion, and EVG staining showed portal vein intimal injury in the model and model recovery groups. In contrast, there was no PVT and the vascular structure was intact in the blank group. Transmission electron microscopy showed collagen fiber bundles in the hepatic sinuses of cirrhotic rats, and hepatocyte mitochondria were heterogeneous in size, with focal aggregation. Portal vein endothelium exfoliation, apoptosis, phenotypic migration of smooth muscle cells to the protoendothelium, and subintimal fibrous tissue proliferation were also detected. No rats in the model recovery group had died 3 weeks after surgery and PVT remained stable. Conclusions　Shedding of the portal vein endothelium, intimal fibrosis, phenotypic smooth muscle cells and migration to the intima are important pathologic findings of PVT in cirrhosis. Intermittent ligation combined with clamping can be used to establish a stable model of PVT in rats with liver cirrhosis, and the pathological changes, including vascular endothelial injury, intima thickening and fibrosis, and slow blood flow, are consistent with the formation mechanism of PVT in liver cirrhosis. Model rats can survive for at least 3 weeks, thus providing a suitable model and survival time for further studies of PVT in liver cirrhosis.

Abstract: Objective　To investigate the establishment and evaluation of an idiopathic pulmonary fibrosis (IPF) mouse model induced by the intratracheal infusion of bleomycin (BLM) of different concentrations. Methods Male C57BL/6J mice were randomly divided into a control group, Model-L group (1.5 mg/kg, BLM), Model-M group (2.5 mg/kg, BLM), and Model-H group (3.5 mg/kg, BLM). An IPF mouse model was constructed by onetime intratracheal infusion of BLM. The general status, body mass, survival rate, and lung coefficient of mice in different groups were compared. Pathological changes in lung tissue, the hydroxyproline content, fibrosis markers and inflammatory factor levels were observed. Results　Compared with the control group, the survival rate decreased and body weight showed a downward trend in the low-, medium-, and high-dose model groups, with significant increases in lung coefficients. Inflammatory infiltration (P<0.01) and collagen deposition (P<0.0001) were observed in the lung tissues of all model groups. Hydroxyproline levels in lung tissue and serum were significantly elevated (P<0.05). The mRNA levels of fibrosis markers α-Sma, Fn1, and Col1a1 were upregulated (P<0.001), with significant increases in corresponding protein expression (P<0.05). The mRNA expression of the inflammatory factor Tgfb1 also increased (P<0.0001). Conclusions　1.5, 2.5 and 3.5 mg/kg BLM can induce an IPF model in C57BL/6J mice. Based on the Results observed for survival rate, body mass, lung coefficient changes, lung tissue gross and pathological changes, and fibrosis-related biomarkers, 2.5 mg/kg BLM is the optimal concentration for inducing an IPF mouse model.

Abstract: Objective　The myocardial injury was induced by hypobaric hypoxia through regulating the expression of various proteins. The expression of proteins was mainly detected by western blot, but the selection of internal reference proteins and their variations have not been systematically studied. Methods　Myocardial injury was induced in a low-pressure, low-oxygen chamber simulating an altitude of 6000 m, for 24 and 72 h. Establishment of the myocardial injury model was confirmed by hematoxylin eosin(HE) staining. Expression levels of internal control proteins, including vinculin, α-tubulin, eukaryotic translation initiation factor-5 (EIF5), β-actin, glyceraldehyde-3phosphate dehydrogenase (GAPDH), cyclophilin B, and cofilin, were detected by Western Blot and total protein expression was detected by Ponceau S and Coomassie Blue staining. An adult mouse cardiomyocytes (AMCMs) injury model was induced by hypoxia for 12 and 24 h and confirmed by terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL staining). Internal control proteins were detected by Western Blot, as in the in vivo model, and total protein expression was detected by Ponceau S and Coomassie Blue staining. Results　A myocardial injury model was established by hypobaric hypoxia for 24 and 72 h, the total protein expression levels remained consistent. The expression of internal control proteins including vinculin, EIF5, β-actin, cyclophilin B, and cofilin was consistent between the control and model groups. Expression levels of α-tubulin were similar in the plain control and 24 h hypobaric hypoxia group, but were significantly lower in the 72 h hypobaric hypoxia group compared with the plain control group. GAPDH expression was significantly higher in the 24 and 72 h hypobaric hypoxia groups than in the plain control group. An AMCM injury model was established by hypoxia for 12 and 24 h. Total protein levels and expression levels of the internal control proteins EIF5 and β-actin were consistent, but vinculin, α-tubulin, GAPDH, cyclophilin B, and cofilin expression levels were higher in both hypoxia groups compared with the normoxic control group. Conclusions　EIF5 and β-actin may be the suitable loading control proteins for studies of hypobaric hypoxia induced myocardial injury using Western Blot. Total protein is also a good choice for hypobaric hypoxia studies.

Abstract: Objective　This study aimed to investigate the effects of sleep interruption (SI) cycles on emotional behavior in ICR mice, and to establish a mouse model of comorbid anxiety and depression induced by SI. Methods　Seventy-two male ICR mice (4 ~ 5 weeks old) were divided randomly into a blank group and a model group. Mice in the model group were subjected to SI stress modeling for 1, 2, and 3 weeks, respectively. After modeling, emotional behaviors were evaluated using open-field, elevated plus maze, light-dark box, marble-burying, and forced-swimming tests. Serum corticosterone levels were detected by enzyme-linked immunosorbent assay. Results　Mice in the model group buried significantly more marbles after 1 week of SI stress, compared with the blank group (P<0.05). After 2 weeks of stress, mice in the model group also showed a significant decrease in the number of crossings in the light-dark box (P<0.05) and a significant increase in the number of marbles buried (P<0.01) compared with the control group. After 3 weeks of stress, mice in the model group showed a significant increase in the number of marbles buried (P<0.05), a significant decrease in the number of crossings in the light dark box (P<0.05), and a significant increase in immobility time in the forced-swim test (P<0.01). Conclusions　ICR mice exhibited significant anxiety-related behaviors after 2 weeks of SI modeling and significant anxiety- and depressive-related behavioral changes after 3 weeks. Three weeks of SI stress can be used to establish a model of comorbid anxiety and depression.

Abstract:Idiopathic pulmonary fibrosis (IPF) is a chronic progressive disease with a short survival time after diagnosis. Current treatments using western medicines have shown poor efficacy and lung transplantation is costly, highlighting the need to find new, safe, and effective treatments. Animal experiments are important for investigating the mechanisms of drug action, and suitable animal models of IPF are required for experimental research. Bleomycin is commonly used to induce IPF models. Traditional Chinese medicine (TCM) has the advantages of multiple therapeutic targets and few adverse reactions, and is gradually gaining attention for the treatment of IPF. Ongoing experimental research is being carried out to verify the specific targets and mechanisms of TCM in the treatment of IPF, utilizing animal models of IPF. This review considers the selection of animal models of IPF, the method used to induce, establish, and evaluate the models, and the interventional effects of TCM. We also summarize the best modeling method for animal models of IPF and the current status of TCM treatments to provide a basis for further scientific research and the clinical treatment of IPF.

Abstract:Organoids have become an important technological platform in cancer research, but simulating the primary tumor tissue structure and function still presents problems. The development of gene-editing technology, especially when combined with tumor organoids, provides a new approach for accurately and comprehensively simulating the in vivo characteristics of tumor models. Introducing specific gene mutations or correcting mutations in tumor organoids through gene-editing technology can allow detailed analysis of the mechanisms of tumor initiation and progression, as well as exploring potential therapeutic targets, accelerating the drug-screening process, and providing new insights for personalized cancer treatment. This article reviews the formation of tumor organoids and the technical aspects of gene-editing strategies, emphasizing their unique applications and prospects in tumor organoids. We also propose that accurately simulating the in vivo microenvironment, promoting the standardization and stability of organoid gene-editing technology, and optimizing the efficiency of gene editing can accelerate the application of organoids in precision medicine research.

Abstract:DNA repair is essential for the successful replication of genetic material and for transcriptional fidelity. Excision repair cross-complementation group 1 (Ercc1) is a structure-specific nucleic acid endonuclease that repairs DNA damage by participating in the nucleotide excision repair and DNA double-strand break repair pathways. The accumulation of various types of molecular and cellular damage over time lead to aging. Defects in Ercc1 are associated with malfunctions in DNA damage repair, Results ing in the accumulation of cellular damage and ultimately inducing aging. This paper summarizes the biological functions of Ercc1 during DNA damage and the phenotypes of Ercc1-deficient mouse models, and discusses the biological effects of Ercc1 in different tissues associated with senescence and age-related degenerative diseases. This review highlights potential intervention targets and provides a theoretical basis for the development of innovative therapeutics, animal models, and drug discovery for senescenceassociated diseases.

Abstract:Chronic pain has emerged as a prevalent medical challenge in contemporary society. Patients suffering from chronic pain frequently develop comorbid psychological disorders, including anxiety, depression, posttraumatic stress disorder, and various psychiatric syndromes. These psychological complications not only affect patients’ pain perception and responses, but may also constitute critical obstacles during pain management interventions. Acupuncture is a long-established clinical practice that has demonstrated remarkable efficacy in alleviating diverse pain types and has shown favorable therapeutic outcomes in ameliorating emotional disturbances such as anxiety and depression. The precise mechanisms underlying acupuncture-induced analgesia and anxiolytic effects, however, remain to be fully elucidated. In this context, it is essential to establish suitable and stable animal models to allow in-depth investigations into the pathogenesis of pain-related emotional disorders and the mechanistic foundations of acupuncture. This article presents a comprehensive review of recent literature regarding the selection of experimental animals, model-establishment method ologies, and behavioral-assessment paradigms pertaining to animal model platforms of chronic pain with comorbid anxiety. We also provide an in-depth discussion of research advancements regarding acupuncture intervention parameters, including needling techniques, acupoint selection, treatment duration, and efficacy evaluation within these animal models. This review proposes comprehensive and reference strategies for constructing preclinical animal models to investigate the mechanisms of acupuncture in managing chronic pain with comorbid anxiety, thus supporting scientific advancements in related research fields.

Abstract:As a chronic progressive lung disease with poor prognosis, the pathogenesis of idiopathic pulmonary fibrosis (IPF) has not yet been clarified, and its treatment is still being explored. Animal models are important tools for studying the pathogenesis and therapeutic effects of diseases. Based on the similarity between animal models and human of IPF in terms of disease phenotype, this paper will review the research progress of IPF animal models in terms of the fibrosis mechanism induced by drugs and environmental factors, histopathological alterations, fibrosis stage, modelling time, etc., and describe the advantages and disadvantages of different animal models, their characteristics and application profiles.

Abstract:The phenol glue modeling method is a widely used modeling technique in animal experimental models of gynecological diseases. Phenol glue is injected into the rat uterus as an adhesive agent, causing chemical burns to the pelvic organ tissues, to construct animal models of pelvic inflammatory disease, cervicitis, endometritis, salpingitis, vaginitis, and other gynecological diseases. The burns induce hyperemia, edema, inflammatory exudation, inflammatory adhesions, and blockages, leading to chronic inflammation. The corrosive and adhesive properties of phenol paste are also used to create animal models replicating gynecological diseases in traditional Chinese medicine, based on the syndrome of dampness and blood stasis. The successful establishment of the phenol glue model provides a crucial experimental basis for studying the pathological mechanisms and treatment method of gynecological diseases.