2023, 31(7):928-934.DOI: 10. 3969 / j.issn.1005-4847. 2023. 07. 012
Abstract:With the increasing age of the population, and a large amount of bone calcium loss, osteoporosis has become a major public health problem. Osteoporosis is a “silent disease”, with a high incidence, high disability, and high death rates, which seriously affect quality of life. There are many animal models of osteoporosis, but there is a lack of relevant TCM disease and syndrome factors. Therefore, it can be combined with models that are highly consistent with clinical practice to make it closer to clinical research. In this article, we systematically review the advantages and disadvantages of various method of osteoporosis modeling, the advantages and disadvantages of animal species selection, the time of modeling, the drugs selection, and the application of assessment indicators to provide a reference for the standardization and improvement of animal model research.
2023, 31(10):1324-1333.DOI: 10. 3969 / j.issn.1005-4847. 2023. 10. 010
Abstract: Objective To study the characteristics and application status of animal models of ovarian cancer and provide guidance for standardized preparation of ovarian cancer models. Methods Using “ovarian cancer” and “animal model” as the main topics in CNKI, Wanfang, VIP, and PubMed databases, the databases were searched for animal experimentation literature on ovarian cancer from establishment of the database to March 1, 2023. A total of 1428 relevant articles were collected. From the experimental animal species, age, experimental modeling method, detection indicators, and other aspects of the summary, we established a database for systematic analysis. Results A total of 178 studies were obtained after screening. Female BALB/ c mice were selected to establish the animal model of ovarian cancer. Most choices were 4 ~ 6 and 6 ~ 8 weeks of age. The most common modeling method was ectopic transplantation, which was mostly subcutaneous injection in the axilla. In terms of modeling method of ovarian cancer in animals, transplantable models were the most used, and ectopic transplantation was more often employed than orthotopic transplantation. Ovarian cancer cell lines used were mostly human. Most detection indexes were the appearance of tumor tissue, tumor pathology, and immunohistochemistry. Conclusions Animal models of ovarian cancer are widely used in ovarian cancer research, but standard preparation and evaluations are lacking. We analyzed the application status of animal models of ovarian cancer in detail through literature collation and data classification analysis to provide a reference to establish standardized animal models of ovarian cancer. BALB/ c mice are the most selected, and human ovarian cancer cell line SKOV3 at 1 × 107 cells/ mL is commonly injected into the subcutaneous axillary area for 10 days. A large number of models can be obtained in a short time with a high tumor formation rate and small individual differences, which provide a reference to study ovarian cancer.
2023, 31(11):1439-1446.DOI: 10. 3969 / j.issn.1005-4847. 2023. 11. 008
Abstract:Objective To study the application of an animal model of glaucoma and provide a reference for improved animal experimental method and models. Methods We searched the CNI and PubMed databases using the terms “glaucoma ”and “animal models” to identify studies related to animal models of glaucoma from 2012 to 2022. The study species, sex, modeling method , and detection indexes were summarized, and a database was established for statistical analysis. Results In total, 400 articles conforming to the criteria were selected. Most of the experimental animals were C57BL/ 6J mice and most of them were male. The most common modeling method were anterior chamber injection-induced, transgenic, and laser photocoagulation-induced models. The most frequent detection indicators included intraocular pressure measurement, histopathology, Western Blot analysis, and immunohistochemistry. Conclusions Numerous method have been used to construct glaucoma animal models, but the intervention of related traditional Chinese medicine factors is less. It is suggested to increase the glaucoma animal model combined with disease and syndrome. This study evaluated different animal models of glaucoma by data mining to provide references for the construction of animal models with high modellingsuccess rates, good reproducibility, and high clinical coincidence, as well as providing ideas for model improvement.