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1 A comparative study of auxiliary lipid-lowering function of Antarctic krill oil preventive and therapeutic administration

sunchanghua , zhuqingfen , wangna , zhaodeming

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[Abstract](115272) [HTML](0) [PDF 0.00 Byte](2092)

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Abstract: To study the effect of auxiliary lipid-lowering function of preventive and therapeutic administration of Antarctic krill oil on experimental hyperlipidemic rats. Methods: We established hyperlipidemia model in SD rats that administrated of Antarctic krill oil for prevention and treatment respectively, then measured and analyzed the serum concentration of total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C). Results: Antarctic krill oil preventive administration can significantly reduce serum TC, LDL-C(p＜0.05), which confirms with the results of liver pathology mutually, while no significant change of serum TG and HDL-C(p＞0.05). There was no significant statistical difference in serum concentration of TC, TG, HDL-C and LDL-C(p＞0.05) and no effect on auxiliary lipid-lowering after Antarctic krill oil therapeutic administration.Conclusion: There was significant effect on auxiliary lipid-lowering and reducing of TC after Antarctic krill oil preventive administration.

2 Current understanding of the intestinal microbiota of piglets

XIA Yao-yao , REN Wen-kai , HUANG Rui-lin , ZENG Ben-hua , WEI Hong , YIN Yu-long

2017, 25(6):681-688. DOI: 10.3969/j.issn.1005-4847.2017.06.018

[Abstract](53147) [HTML](0) [PDF 3.50 M](4003)

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The role of intestinal microbiota in mammals and humans are gaining increasing attention.The health of piglets requires a dynamically balanced intestinal microbiota.However,there are temporal and spatial changes in the distribution and composition of microbiota from the esophagus to the rectum during the life cycle of the pig.The intestinal microbiota has various beneficial functions in pig,like nutrient metabolism,intestinal mucosal barrier,immune responses and pathogen infection.A variety of factors play an important role in the formation and stabilization of intestinal microbiota,including the ways of delivery (vaginal or caesarean),the diet during infancy (breast milk or formula feeds) and the usage of antibiotics or antibiotic-like molecules.In this review,we mainly discussed the relationship between intestinal microbiota and the intestinal health of piglets from the aspects of the composition and colonization of intestinal microbiota,as well as the functions and influencing factors of intestinal microbiota,so as to further understanding the importance of intestinal microbiota in intestinal function of piglets.

3 Application of Chinese Bama minipigs in medical research：a literature review

PANG Lin-lin , ZHANG Hui-yong , YANG Guan-lin

2014, 22(1):94-98. DOI: 10.3969/j.issn.1005-4847.2014.01.020

[Abstract](10980) [HTML](0) [PDF 711.74 K](5532)

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As one of the major domestic minipig strains, Guangxi Bama minipigs have the following characteristics: genetic stability, fecund species, lighter weight, large area of body surface covered with white hairs, many tissues and organs and biochemical indicators similar to those of humans and so on. All these characteristics make them being widely applied in medical research. Because of the similar anatomy and physiology in cardiovascular system, Bama minipigs have been used in the research of cardiovascular system. In our country, Bama minipigs are used for establishing models of cardiovascular diseases such as myocardial ischemia, patent foramen ovale and so on. Pigs are omnivore-animals and similar to human beings in lipid metabolism, so they can be used in the study of endocrine diseases. Bama minipigs have been used in modeling, genetic susceptibility and complication prevention of diabetes. Bama mini-pigs' digestive system is similar to that of humans, so they can be used as a model of digestive system diseases including chronic pancreatitis, rupture of colon and bilioenteric anastomosis. The aspect that large area of their body surface is covered with white hairs except their head and tail makes them ideal model of skin wound and healing of burns. Bama minipigs can be used for stomatological research for their similarity to human beings in the structure of the teeth and large crack. Bama minipigs have been applied for the research of pulp necrosis and the way of maxillary expansion. They are similar to human beings in anatomy, physiology and pathology, which makes them an appropriate provider for zenotransplantation. In the research of traditional Chinese medicine, Bama minipigs has been used as liver, spleen and femoral arteriovenous fistula hemorrhagic model to study curative effect and mechanism of traditional Chinese preparation.

4 Comparison of the tumor growth and pulmonary metastasis in mice transplanted with different number of luciferase-labeled mouse breast cancer 4T1 cells

LI Xiao-ying , MA Yuan-wu , ZHANG Xu , ZHANG Lian-feng

2012(1):14-17. DOI: 10.3969/j.issn.1005-4847.2012.01.004

[Abstract](10648) [HTML](0) [PDF 0.00 Byte](68)

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ObjectiveTo study the tumor growth and pulmonary metastasis in mice transplanted with different number of luciferase-labeled mouse breast cancer cells, and to provide important information for drug screening and cancer mechanism research. MethodsThe vector containing luciferase gene was constructed and transfected into mouse breast tumor cell line 4T1 cells and selected with G418 to obtain stable Luc-expressing clones. The cells in logarithmic phase was collected and diluted to 1×10⁸,5×10⁷,2×10⁷ and 1×10⁷ cells/mL. Then 0.1 mL cell suspension was inoculated into the second mammary fat pad on the right side of normal BALB/c mice to establish the tumor models. Their tumorigenesis and metastasis were analyzed in vivo.ResultsThe stable Luc-expressing cell lines were obtained which had no obvious difference with parental cells in tumorigenicity. The whole-body optical imaging found that tumor could be formed after the cells were inoculated orthotopically. After 28 days, the tumor sizes were similar among the four concentrations, while there were two mice each died at 5×10⁶ and 1×10⁷ cells concentrations. After 31 days, the tumor metastasis showed different degree among the four concentrations. The metastasis became more seriously with time passed. However, after 42 days, deaths of mice occurred in succession.ConclusionsThe number of 1×10⁶ cells is the best concentration, not only induces obvious pulmonary metastasis but also shows a survival time longer than 45 days, compared with that induced at other higher concentrations. 

5 Establishment of a mouse model of H7N9 avian influenza A virus infection

ZHU Hua , XU Li-li , BAO Lin-lin , DENG Wei , CHEN Ting , LU Qi , LI Feng-di , YUAN Jing , XU Yan-feng , HUANG-Lan , LI Yan-hong , LIU Jiang-ning , YAO Yan-feng , YU- Pin , YONG Wei-dong , WEI Qiang , ZHANG Lian-feng , QIN Chuan

2014, 22(1):18-21. DOI: 10.3969/j.issn.1005-4847.2014.01.004

[Abstract](9896) [HTML](0) [PDF 739.37 K](3774)

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Objective The aim of this study was to establish a mouse model of H7N9 avian influenza A virus infection. Methods Seventy SPF healthy female BALB/c mice were used in this study. A/Anhui/1/2013 (H7N9) avian influenza virus was administered by intranasal instillation to BALB/c mice, inoculated in a dose of 50 μL 1×10⁸, 1×10⁷ or 1×10⁶ TCID₅₀, respectively, 10 mice in each group. Other 30 mice were used for virus titration and pathological examination. Ten mice were given saline as control group. The changes of body weight, clinical signs and death of the mice were observed every 24 h from 1 to 14 d. Blood and several organ samples were taken for pathological examination, and avian influenza A (H7N9) virus was detected with virus titration and immunohistochemistery (IHC). Results The mice developed typical clinical signs including body weight loss, ruffled fur and humped back. The peak of virus shedding from respiratory tract was observed on 2 days post inoculation (d.p.i.), and histopathological examination observed interstitial pneumonia. The virus was also detected in the brain, liver, spleen, kidney and intestine from inoculated mice. The inoculation of H7N9 virus elicited seroconversion titers up to 160. There was reduction of lymphocytes and increase of neutrophils in the blood. Conclusions The mouse model of H7N9 avian influenza virus infection established in this study show similar signs of human avian influenza. Therefore, it provides a useful working basis for research of the pathogenesis, drug development, and vaccine evaluation of this disease.

6 Evaluation of the efficiency and toxicity of AAV9-SERCA2a gene transfected by different cardiac gene delivery methods at the same titer in rats

徐晓霞 , 周祁娜 , 姜涛 , 王红丽 , 王欢 , 季萌 , 王凌鹏 , 张广伟 , 侯月梅

2013, 21(3).

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Objective To assess the efficiency and toxicity of adeno-associated virus serotype 9 (AAV9) mediated sarcoplasmic reticulum Ca2+ATPase 2a (SERCA2a) gene transferring to myocardium in SD rats by different cardiac gene delivery methods. Methods The AAV9-SERCA2a-EGFP virus victor system was constructed in vitro successfully. According to the three cardiac gene delivery methods, 90 normal SD rats were randomly divided into three groups: TVI (Tail vein injection，TVI)group, IMI（Intramyocardial injection，IMI）group, and IPI (Intrapericardial injection，IPI)group. Each group was respectively transferred with AAV9-SERCA2a-EGFP, AAV9-EGFP, NS 200µL at the titer of 1×1011 vg/mL using the appropriate method. After 30 days of gene delivery, the expression of EGFP in the tissue of heart, liver and kidney was observed by inverted fluorescence microscopy. Western blotting was performed to detect relative expression levels of SERCA2a gene in rats' tissue. Surface 12-lead ECG was used to record the incidence of arrhythmia. HE staining observed the histopathological changes. The changes of cardiac function was measured by echocardiogram. Blood chemistry indicators were used to assess the changes in liver and kidney. Results Left ventricular could observed a large number of green fluorescent in three groups and IMI group of green fluorescent confined in the injection point, liver and kidney were seen faint green fluorescence in three groups; The relative expression of SERCA2a protein of myocardium in the three groups was significantly higher than liver and kidney (P<0.01), and the myocardial relative expression of SERCA2a protein in TVI group and IMI group was significantly higher than IPI group (P<0.01). ECG was normal in TVI group and IPI group, but frequent premature ventricular beats were observed in two rats of IMI group.There were no significant difference in cardiac function, histopathology and blood chemistry indicators between transferring AAV9-SERCA2a-EGFP, AAV9-EGFP and transferring NS (P>0.05). Conclusion Transfected with a titer of 1×1011 vg/mL AAV9-SERCA2a gene, the efficiency in TVI group and IMI goup was better than IPI goup, and the toxicity in TVI group and IPI goup was lower than IMI group. Considering the evaluation of efficiency and toxicity by 1×1011 vg/mL AAV9-SERCA2a gene delivering in three methods, the TVI method is better than IMI and IPI method.

7 Differential analysis of the bacterial community composition in mouse feces and intestinal contents

WU Ya-qi , ZHONG Gen-shen , WU Min-na

2015, 23(3):249-255. DOI: 10.3969/j.issn.1005-4847.2015.03.006

[Abstract](8995) [HTML](0) [PDF 1.18 M](6876)

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Objective To explore the objectivity and scientificity of fecal sampling, and to provide reference for investigating the relationship between intestinal microbes and diseases. Methods Terminal restriction fragment length polymorphism, degeneration gradient gel electrophoresis and real time fluorescent quantitative PCR techniques were applied to differentially analyze the bacterial community composition and abundance of intestinal contents and feces taken from different sites of BALB/c mouse intestine. Results The predominant T-RFLP fragments (T-RFs) in feces in the rectum and colon were 244 bp, 255 bp and 449 bp, however, those in feces of the small intestine including duodenum, jejunum and ileum were 60 bp, 73 bp, 261 bp, 268 bp and 272 bp, and with a larger variation of the bacterial community composition in various parts of the small intestine. The bacterial abundance in the contents of duodenum and jejunum were 6.9 log (copies)/g and 8.3 log (copies)/g, fewer than in the other parts of the intestine, while the bacterial abundance in the feces was as high as 11.8 log (copies)/g, being about 2 times higher than that in the duodenum and jejunum (P<0.05), and similar to that in the ileum and colon content (P>0.05). Conclusions The inter-mouse variations of bacterial community composition in the large intestine contents are small. The bacterial composition and abundance are similar suggest that studies on the relationship between large intestine especially colorectal microbiota and diseases may be conducted via fecal sampling.

8 Establishment of a method for culture in vitro of peripheral blood monocytes from rhesus macaque

SANG Ming , DAI Ming , ZHOU Li , LIU Jin-biao , GUO Ming , MA Tong-cui , XIAO Qian-hao , HO Wen-zhe

2015, 23(1):18-24. DOI: 10.3969/j.issn.1005-4847.2015.01.004

[Abstract](8985) [HTML](0) [PDF 985.49 K](4176)

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Objectives To establish a simple, inexpensive and efficient technique for in vitro culture of monocyte-derived macrophages (MDM) from rhesus macaques of Chinese origin. Methods Peripheral blood of healthy rhesus macaques (Macaca mulatta) were obtained in heparinized vacutainer collection tubes. Peripheral blood mononuclear cells (PBMCs) were isolated from blood by Ficoll gradient centrifugation. Serum was isolated from peripheral blood of the autologous animals. PBMCs were plated in 48-well-plate (3×10⁶ cells/well) or 96-well-plate (0.8×10⁶ cells/well) for 24 h. After removal of non-adherent cells from the culture, monocytes were cultured in RPMI 1640 supplemented with different proportions (2%, 4%, 8%, 10%) of autologous serum or fetal calf serum (FCS) for 7 days. To examine the biological function of MDM, lipopolysaccharide (LPS) was added to MDM culture to determine inflammatory cytokine production. Also, MDM cultures were tested for the susceptibility to simian immunodeficiency virus (SIV) or simian-human immunodeficiency virus (SHIV) infections. Results The cell cultures with RPMI1640 containing 2% autologous serum yielded the best results with regard to macrophage morphology, the response to LPS stimulation and susceptibility to SIV or SHIV infection. The purity of adherent macrophages under condition of 2% autologous serum culture was higher than 96%. Conclusions RPMI 1640 with 2% autologous serum is suitable for culture in vitro of peripheral blood monocytes from rhesus macaques. This technique is simple, inexpensive, no need for growth factor and highly effective to obtain adherent and well differentiated macaque monocytes. Therefore, this method provides an important tool for culture of macaque AIDS viruses and for related immunological research.

9 Sequence analysis of the complete mitochondrial genome and molecular evolution of Cricetulus griseus

SONG Guo-hua , LIN Qiang , YUE Wen-bin , LIU Tian-fu , HU Song-nian

2012(1):70-75. DOI: 10.3969/j.issn.1005.4847.2012.01.005

[Abstract](8576) [HTML](0) [PDF 0.00 Byte](45)

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ObjectiveTo obtain the nucleotide sequence of the complete mitochondrial DNA (mtDNA) of Cricetulus griseus to provide molecular data for mitochondrial disease models. MethodsThe complete mitochondrial genome of Cricetulus griseus was sequenced with 27 primers and their PCR amplications, the genomic structural characteristics and gene mapping were analyzed, and to discuss the phylogenetic relationship among 5 rodent species whose complete mitochondrial genome sequences are available in the published databases. ResultsThe complete genome contains 16 283 base pairs and encodes 37 genes,including 13 protein-coding,2 ribosomal RNA and 22 transfer RNA genes. The base composition for the four nucleotides is 33.53 % A, 30.50% T, 12.98 %G and 22.80% C. ConclusionsThe genome of Cricetulus griseus is very similar to that of other vertebrate mitochondrial genomes. Some of the protein-coding regions and the tRNA genes are highly homologous to those of other rodents. The evolution tree built from the homology of 5 kinds of laboratory animals is consistent with the traditional taxonomic results.

10 Cloning and sequence analysis of tyrosinase gene in Cricetulus barabensis and albino mutant Cricetulus barabensis

ZHAO Yan-bin , SUN Zhao-zeng , YE Hua-hu , WANG Dong-ping , HU Zhong-ming , ZENG Lin

2012(1):5-9. DOI: 10.3969/j.issn.1005-4847.2012.01.002

[Abstract](7856) [HTML](0) [PDF 0.00 Byte](53)

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ObjectiveIn order to reveal the molecular mechanism of the albino mutant Cricetulus barabensis, the tyrosinase gene of Cricetulus barabensis and the albino mutant Cricetulus barabensis was cloned, sequenced, and comparatively analyzed. MethodsThe Tyrosinase cDNA was amplified from the total RNA of the skin of Cricetulus barabensis and its albino mutant Cricetulus barabensis by reverse transcriptase (RT-PCR). According to the conservative region of tyrosinase gene of mice and rats, three pairs of primers were designed to link three fragments to a long epitope by overlap extension. Then the three fragments were amplified and sequenced and finally spliced into the complete sequence. The mutation site was identified through DNA matching software.ResultsThe tyrosinase genes of Cricetulus barabensis and the albino mutant Cricetulus barabensis were cloned and the mutation site was measured by DNA sequence analysis software. The results showed that they have the same coding region and there is no mutation.ConclusionsThe reason of the albino trait of the albino mutant Cricetulus barabensis is different from that of albino trait known in mice, not by the mutation of tyrosinase gene coding region, and reqires further investigation.

11 Research progress in animal models and mechanism of depression

HAN Yuan-yuan , DAI Jie-jie

2016, 24(3):321-326. DOI: 10.3969/j.issn.1005-4847.2016.03.020

[Abstract](7570) [HTML](0) [PDF 774.13 K](11662)

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The incidence of depressive illness is high worldwide, and the inadequacy of currently available drug treatments contributes to the significant health burden associated with depression. Animal models of depression used as the main methods to study the pathogenesis mechanism and select effective drugs receive increasing concerns. Current popular models of depression creatively merge ethologically valid behavioral assays with the latest technological advances in molecular biology. In this context, this study aims to review the animal models of depression and pathogenesis related with face validity, construct validity, and predictive validity of these models. These models include stress-induced models, injury-induced models, drug-induced models and transgenic models which all mimic the depression symptoms of human to some degree and are of great value for developing new antidepressant drugs and studying the pathogenesis of this disease.

12 Determination and analysis of blood biochemical parameters of wild and breeding rhesus macaques in Anhui Province

XU Yu-rui , LI Jin-hua , SUN Bing-hua , XIA Dong-po , ZHU Yong , WANG Xi

2014, 22(1):87-90. DOI: 10.3969/j.issn.1005-4847.2014.01.018

[Abstract](7443) [HTML](0) [PDF 701.51 K](3833)

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Objective To determine the blood biochemical parameters of wild and cage reared rhesus macaques in Anhui Province, and compare the differences between the two kinds of macaques and B virus (BV) positive and negative infection. Methods Fourteen blood biochemical indexes of Anhui rhesus macaques were measured with an automatic blood biochemical analyzer. The differences of biochemical parameters between wild and breeding, and BV positive and negative infection were analyzed. Results The blood biochemical indexes of males were higher than those of females. ALP, TG and GGT showed significant difference between males and females of wild macaques. ALP, ALB, Ca, TG, Cr and GGT showed significant difference between males and females of breeding macaques. The biochemical indexes of BV positive infected monkeys were higher than that of BV negative infected monkeys. Conclusion The blood biochemical parameters are different between wild and breeding macaques, males and females, and BV positive and negative infected monkeys.

13 Establishment of a mouse model of aorta dissection induced by β-aminopropionitrile drinking combined with angiotensin II infusion

LIU Yu-ting , GAO Yan-xiang , WANG Shan-shan , REN Wei , SUN Wei-liang , YU Chang-an , ZHENG Jin-gang

2017, 25(4):399-403. DOI: 10.3969/j.issn.1005-4847.2017.04.010

[Abstract](7053) [HTML](0) [PDF 3.32 M](5701)

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Objective To establish a mouse model of aorta dissection (AD) by β-aminopropionitrile (BAPN) in drinking water + subcutaneously pumped angiotensin Ⅱ (Ang Ⅱ) infusion. Methods Forty 3-week-old C57B1/6J male mice were randomly divided into two groups. All animals received 0.1 g/kg/d BAPN in drinking water for 4 weeks. Then the BAPN drinking + saline infusion group and BAPN drinking + Ang Ⅱ infusion group received continuous saline or Ang Ⅱ (1,000 ng/kg/min) infusion, respectively, via subcutaneous osmotic minipump for 72 hour. The mice were restricted in a noninvasive computerized tail-cuff system and their arterial systolic blood pressure and heart rate were monitored. Autopsy was performed if a mouse died during the experiment. At the end of the experiment, mice were sacrificed by injection with an overdose of sodium pentobarbital and the aortas were harvested. The formation of aortic false lumen was observed by pathology using hematoxylin-eosin staining. Results The overall incidence of AD in the BAPN drinking administration +Ang Ⅱ infusion group was 95%, whereas the incidence of AD in the BAPN drinking administration +saline infusion group was only 5%. The mortality from dissecting aneurysm rupture was 24% in the BAPN drinking administration +Ang Ⅱ infusion group during the experiment. Pathological examination of the aortic cross-sections clearly showed the formation of blood-filled false lumens induced by Ang Ⅱ. Conclusions A mouse model with high incidence of aortic dissection is successfully established.

14 Oxidative stress in the rat models of Parkinson's disease dyskinesia with Yin deficiency stirring wind pattern, and the interventional effect of compound formula Rehmannia on the disease condition

TENG Long , HONG Fang , HE Jian-cheng

2015, 23(1):25-29. DOI: 10.3969/j.issn.1005-4847.2015.01.005

[Abstract](6559) [HTML](0) [PDF 756.47 K](4007)

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Objective To explore the oxidative stress in Parkinson's disease dyskinesia with Yin deficiency stirring wind pattern, and the interventional effect of compound formula rehmannia on the disease condition. Methods One hundred and twenty healthy male SD rats (body weight 180-200 g) were randomly divided into four groups: the control group, sham-operated group, model group, and Rehm group. The rat model with Parkinson's disease (PD) was established by injection of 6-hydroxyl dopamine to destroy the substantia nigra using the brain stereotaxic apparatus. Dyskinesia was induced in the PD rats by injection of levodopa into the brain. The dyskinesia PD model rats received intraperitoneal injection of levodopamine 50 mg/kg and benserazide 12.5 mg/kg for 2 weeks to induce Yin deficiency stirring wind pattern. The rats of Rehm group were given gastric gavage of compound formula rehmannia 2 mL/kg once a day for 4 weeks or 6 weeks. At the end of experiment (4 weeks and 6 weeks), neurobehavioral examination was performed, and the contents of SOD, MDA, GSH, and GSH-Px in the striatum tissue were assessed by colorimetry. Results In the LID group, the contents of SOD, GSH, GSH-Px in the striatum tissue were significantly lower than those in the normal control group and sham-operated group, and the malondialdehyde (MDA) content showed an increasing trend. In the compound rehmannia prescription group, the concentrations of SOD, GSH, GSH-Px in the striatum tissue showed an increasing trend, even more obvious along with the time course, and the malondialdehyde (MDA) content showed an decreasing trend, also even more obvious along with the time course. Conclusions The compound rehmannia prescription can improve the oxidative stress in rat models of Parkinson's disease dyskinesia with Yin deficiency stirring wind pattern. The interventional effect of compound rehmannia prescription may be mediated by scavenging free radicals and reducing the damage to the cells, thus, to alleviate the symptoms of Parkinson's disease dyskinesia.

15 Exploration of the establishment of a rat model of chronic diabetic skin ulcer simulating the traditional Chinese syndrome

LI Yuan , ZHAO Guang-ming , DONG Jian-xun , LU Guang-lin , ZHANG Xu-hui , WANG Le-ping

2014, 22(1):63-66. DOI: 10.3969/j.issn.1005-4847.2014.01.012

[Abstract](6552) [HTML](0) [PDF 718.78 K](4969)

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Objective To observe the effects of different modeling methods on the wound morphology and healing time of chronic skin ulcers in diabetic rats. Methods SD rats (n=50) were randomly divided into five groups, 10 rats in each group: skin defect group (group QS: dermal deficiency), diabetes group (group DM: STZ injection+skin excision), diabetes plus Staphylococcus aureus group (group DMJJ: STZ injection+skin excision+bacterial infection), diabetes plus hydrocortisone group (group DMJS: STZ injection+skin excision+hydrocortisone intervention) and diabetes plus hydrocortisone and implantation of foreign body group (DMYW group: STZ injection+skin excision+hydrocortisone intervention + foreign body embedded). The rats were measured for body weight and wound healing every day, and blood glucose after stable diabetes once a week. The rats were sacrificed 12 days later and the skin lesions were examined by histopathology.Results The healing rate of the DMYW group was significantly slower than that in the other groups (P<0.01). At 12 days after modeling, the healing rate of the DMYW group was significantly lower than that of the remaining groups (P<0.01), while the healing rates were not significantly different among the remaining groups.Conclusions The modeling method of DMYW group can show skin wounds similar to the clinical characteristics of "Yin syndrome", and the addition of foreign body implantation significantly prolongs the rat skin healing time.

16 Study of Genetic Polymorphism of Plasma Prealbumin, Transferrin, MDH and ADH from Laboratory-Bred Rhesus Monkeys

Huang Ren , et al

1993(1).

[Abstract](6496) [HTML](0) [PDF 0.00 Byte](40)

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Genetic polymorphism of two plasma proteins prealbumin ( PA ) and transferrin ( Tf ) , and two plasma isozymes, malate dehydrogenase ( MDH) and alcohol dehydrogenase ( ADH ) from 118 laboratory-bred Rhesus monkeys ( M.mulatta ) was analysed by thin-layer polyacry lamide gel electrophoresis.The result suggested that ADH showed the monomorphism, the PA.Tf and MDH showed the polymorphism.The electrophoric pattern of PA can be divided into 7 phenotyp s, that is AA, AB, AC, AD and BB, CC. EE. The genes frequencies of A, B, C, D and E were 0.343, 0.072, 0.042, 0.009 and 0.081 respectively .The Tf can be divided into 17 phenotypes, that is CC, DD, EE, FF, GG, CD, CE, CG, CH, DE, DF, DG, EF, EG, EH, FG.The genes frequencies of C, D, E, F, G and H were 0.064, 0.38, 0.133, 0.111, 0.244, and 0.014 respectively. The MDH isozyme can be divided into MDH1-1 and MDH2-1 two phenotypes MDH1 and MDH2 frequencies were 0.953 and 0.042. The experimen tal results were compared to those of previously reported experiments.

17 Comparison of the rat models of liver cancer spleen deficiency established by Dachengqi and Xiaochengqi decoctions

SUN Bao-guo , XIANG Ting , LI Yu-long , CHEN Ze-xiong

2014, 22(2):20-25. DOI: 10.3969/j.issn.1005-4847.2014.02.005

[Abstract](5222) [HTML](0) [PDF 801.31 K](3459)

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Objective To explore the establishing methods and differences of rat models of spleen deficiency and spleen deficiency liver cancer using the traditional Chinese medicine Dachengqi and Xiaochengqi decoctions.Methods Spleen-deficiency rat models were developed by multifactor methods: bitter-cold purgation (Dachengqi or Xiaochengqi decoction), cold-wet environment, tiredness, and fasting on alternate days for 30 days. Seven days after spleen-deficiency modeled,liver cancer in the spleen-deficiency rats and normal rats was developed by subcutaneously inoculation of Walker-256 carcinoma cell line in nude mice and then transplanted into rat livers. Liver cancer models were observed for 35 days. Sixty 3-week old male Wistar rats were randomly distributed into 4 groups: normal group, liver cancer model group, and Dachengqi and Xiaochengqi decoction groups. Degree of spleen deficiency, changes of the body-weight, survival time and tumor formation were recorded. Results Spleen deficiency rat models were successfully established. The weight gain of rats in the spleen-deficiency groups was significantly inhibited (P<0.01), and during the first 20 days (but not later) the average body weight of the Dachengqi decoction group was significantly higher than that of the Xiaochengqi decoction group (P<0.05). Spleen-deficiency scores of rats in the Xiaochengqi and Dachengqi groups were higher than those in the blank tumor group, especially in the Xiaochengqi group (P<0.01). The total tumor formation rate was 91.1% and 80% in the blank tumor groups, and 93.3% in both Xiaochengqi and Dachengqi groups, respectively. The average survival time of Xiaochengqi group was lower than that of the blank tumor and Dachengqi groups (P<0.01 and P<0.05). The cumulative survival rate of the Xiaochengqi group and rats with a higher spleen-deficiency score was lower than that of the other groups (P<0.05). Conclusions Xiaochengqi decoction may induce spleen deficiency more seriously than Dachengqi decoction, and spleen deficiency may be an important unfavorable prognostic factor for rat models of liver cancer.

18 Progress in Research on Animal Models of Hemorrhoids

MA Zong-guo , JIANG Nan , YU Meng-yao , XU Xiao-yan , CAO Ding-zhi , LUO Xia

2010(5).

[Abstract](5186) [HTML](0) [PDF 0.00 Byte](168)

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随着对痔疮新药药效评价的需求,痔疮模型的建立有了初步的发展.目前,大鼠、小鼠、家兔、卷尾猴等动物已被成功的用于痔疮模型的建立.所用方法主要有:巴豆油法、醋酸法、感染法、创伤法、静脉阻断法等,动物模型的成功建立和合理应用将有利于推动痔疮新药的研发,本文将现有痔疮模型创建的原理和方法进行了归纳、总结.

19 Hepatitis B Virus Acute Infection and High-replication Tree shrew Model by Transduction with a Recombinant Adeno-associated Virus 8 Carrying 1.3 Copies of HBV Genome

曾扬 , 孙世惠

2013, 21(3).

[Abstract](4414) [HTML](0) [PDF 0.00 Byte](314)

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【Abstract】 Objective To establish a tree shrew model of hepatitis B virus acute infection by the injection of a recombinant adeno-associated virus 8 vector carrying 1.3 copies of HBV genome (ayw subtype)(rAAV8-1.3HBV) into the liver of tree shrews. Methods The serum and liver tissues of tree shrews were collected at indicated times after the i.v injection of rAAV8-1.3HBV. The HBsAg、HBeAg、HBsAb、HBeAb、HBcAb、ALT and HBV virus load were measured by ELISA and real-time PCR respectively. The expression of HBcAg and the pathological changes in liver were also detected after the rAAV8-1.3HBV infection. Results The HBsAg and HBeAg in serum were all positive 2 weeks after the injection and the HBcAg positive hepatocytes were even detected in the liver 55days after rAAV8-1.3HBV injection. The HBV DNA copies reached 104 -105 in liver on the day55 and could be detected in serum for over one month after rAAV8-1.3HBV infection. Mild pathological changes were observed with ALT kept rising after rAAV8-1.3HBV injection. Conclusion Acute HBV infection model in tree shrew was developed successfully by the administration of rAAV8-1.3HBV. Our study contributes to the establishment of a novel and facility animal model.

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