As a model organism, the zebrafish is widely used in life science research. However, its systematic integration into undergraduate experimental teaching systems requires further development. Based on the training objective of cultivating research orientation, systematic thinking, and innovation ability in undergraduates, this study designed a comprehensive zebrafish experimental course focused on the functional evaluation of anti-fatigue and anti-wrinkle effects. The course transformed industry consortium standards (T / ZHCA 014-2022) and established scientific research models into comprehensive experimental projects suitable for undergraduate education. It effectively fostered scientific thinking and practical skills by guiding students through the entire process of experimental design, model establishment, data collection and analysis, and academic report writing. Teaching practice has demonstrated that this experimental teaching model not only significantly enhanced students’ experimental operational skills, data analysis capabilities, and teamwork, but also achieved the teaching goals of integrating research into teaching and linking theory with practice. The teaching effectiveness evaluation demonstrated significant improvements in students’ abilities in experimental operations, data analysis, and scientific research thinking (P<0. 05), with course satisfaction exceeding 95%. This model provides a replicable and scalable practical example for implementing innovative experimental teaching involving advanced model organisms at the undergraduate level, representing a valuable exploration for undergraduate teaching quality improvement projects.