Traditional basic medical experimental teaching struggles to meet the demands of cultivating medical students’ practical abilities and innovative literacy. Using physiology experiments as an example, this study established a “trinity” experimental teaching model integrating teaching content reform, evaluation system innovation,and ideological and political elements. The model aims to systematically train clinical medical reserves with standardized operational skills, ethical awareness, and cross-species comparative analysis capabilities. By systematically revising the teaching syllabus, three-stage progressive teaching content was designed involving classical animal, functional human, and independent design experiments. The “ one instrument, one code” technology was employed to ensure animal welfare and operational standards, while ethical education centered on the 3R principles and clinical medical thinking were integrated. This model achieves an organic fusion of basic clinical research capabilities and establishes a multidimensional formative evaluation system. Practice has shown that this model effectively enhances students’ comprehensive practical abilities and innovative literacy, providing a pathway for the construction of medical education systems and the cultivation of future medical talents.