Objective To set up an assay method of heart rate and noninvasive blood pressure determination in tree shrews，the data will be valuable reference values for developing tree shrews as an experimental animal. Methods 180 tree shrews were randomly divided in three groups，which included wild adult group, F1 generation artificial breeding adult and juvenile groups. Each group included 60 animals ( half female and male). The smart noninvasive blood pressure monitor mouse instrument（BP-98A）was used to measure HR(heart rate), SBP (systolic blood pressure), DBP (diastolic blood pressure) and MBP (mean arterial pressure). Results The heart rates of wild adult tree shrews, artificial breeding adult and juvenile tree shrews were 394.33?7.74BPM, 351.61?2.76BPM, 378.19?9.04BPM, respectively. There were be significant differences between wild adult and artificial breeding adult tree shrew groups (P<0.05). The SBP, DBP and MBP of artificial breeding adult group were lower than those of juvenile group, and there were great significant differences between two groups(P<0.01). There were no differences between wild adult and artificial breeding adult groups in SBP, DBP and MBP(P> 0.05). Conclusion We have established a technology for noninvasive blood pressure determination and obtained the reference values of heart rate and blood pressure from wild adult, artificial breeding adult and juvenile tree shrews. It will enrich the data of physiology in tree shrews, which provides scientific reference for studying on the animal experiment.