Objective: To apply electronic tags (RFID) in Biosafety Level II (BSL-2) laboratories and analyze their safety and functionality. Methods: RFID tags were subcutaneously implanted into 21 mice. Tag safety was assessed longitudinally: blood samples were collected into anticoagulant tubes at 3, 21, 30, 56, and 84 days post-implantation for blood routine tests; serum samples were obtained at 3, 7, and 28 days for assessment of hepatic function (alanine aminotransferase [ALT], aspartate aminotransferase [AST]) and renal function (urea). At 3, 7, and 14 days post-implantation, mice were humanely euthanised and spleens were harvested for flow cytometric analysis of T- and B-lymphocyte populations. At 7 and 28 days, the hearts, livers, spleens, lungs, and kidneys were collected from the mice after euthanasia for histopathological examination using haematoxylin and eosin (H&E) staining. Additionally, RFID functionality was concurrently monitored: signal acquisition and recognition sensitivity were evaluated at baseline (day 0, pre-implantation) and across multiple post-implantation time points: 3 days, 7 days, 14 days; 4 weeks, 8 weeks, 12 weeks, 16 weeks.?Results: Implantation of RFID tags induced no statistically significant alterations in murine physiological or biochemical parameters, including hepatic and renal function markers. Histopathological examination revealed no notable tissue lesions attributable to tag implantation. Within the BSL-2 facility, RFID signals were reliably acquired under routine negative-pressure containment conditions, indicating that the laboratory’s environmental shielding did not impair tag sensitivity. Furthermore, as all materials exiting the BSL-2 system must undergo autoclaving (standard high-temperature, high-pressure sterilisation), the study confirmed that RFID tags retained full functional sensitivity following autoclave treatment. Conclusion: The safety and effectiveness of electronic tags were evaluated in a BSL-2 laboratory, and it was found that electronic tags can be used normally in this special experimental environment. These findings support the reliable integration of RFID technology in biosafety laboratories and provide a validated foundation for establishing RFID-based experimental animal traceability systems.