心房钠尿肽对基因敲除小鼠移植黑色素瘤生长的抑制作用
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(广东药科大学,基础学院血管生物学研究所,广州 510006)

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R-33

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国家自然科学基金(31500966,81773095);广州市科技计划项目(201607010135);广东省科技计划项目(2015A020211029,2014A020212314)


Inhibitory effect of atrial natriuretic peptide (ANP) on the tumor growth of ANP knockout mouse and C57BL / 6J mouse models of subcutaneous xenotransplanted melanoma
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(Vascular Biology Research Institute, School of Basic Course, Guangdong Pharmaceutical University, Guangzhou 510006, China)

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    摘要:

    目的 探讨心房钠尿肽(atrial natriuretic peptide, ANP) 对黑色素瘤生长的作用?方法 将体外培养的黑色素瘤B16F10 细胞分别接种于ANP 敲除小鼠和C57BL/6J 小鼠的皮下,建立黑色素移植瘤模型,从第7 天开始测量肿瘤的长径和短径,计算肿瘤的体积;接种肿瘤后的第12 天断颈处死小鼠,分离移植瘤并称重?肿瘤组织石蜡包埋后,用免疫组化检测肿瘤细胞增殖和微血管密度?结果 ANP 敲除小鼠的肿瘤体积明显比C57BL/6J小鼠小,肿瘤重量也明显轻?ANP 敲除小鼠的肿瘤组织中细胞增殖数目和微血管密度也明显比C57BL/6J 小鼠少?结论 ANP 缺失可能通过抑制肿瘤细胞增殖和微血管形成而明显地抑制了黑色素瘤的生长?

    Abstract:

    Objective To study the effect of atrial natriuretic peptide (ANP) deletion on melanoma growth. Methods A subcutaneous xenotransplanted melanoma model was established in ANP knockout mice and C57BL/6J mice. The tumor volume was measured at the sixth day after establishment of the subcutaneous transplantation. Tumor cell proliferation and tumor-induced angiogenesis were detected by immunohistochemical staining. Results The volume and weight of xenotransplanted melanoma were significantly decreased in the ANP knockout mice compared with those in the C57BL/6J mice. The proliferative tumor cells and microvessel density were significantly decreased in the tumor tissues of ANP knockout mice compared with those in the C57BL/6J mice. Conclusions ANP deletion significantly suppresses xenotransplanted melanoma growth through inhibiting the tumor cell proliferation and angiogenesis.

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亓翠玲,曹静桦,何亚军,李梦诗,李倩明,杨扬,王丽京.心房钠尿肽对基因敲除小鼠移植黑色素瘤生长的抑制作用[J].中国比较医学杂志,2018,28(4):15~18.

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  • 收稿日期:2017-09-19
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  • 在线发布日期: 2018-05-22
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