姜黄素在大鼠创伤性脑损伤模型中的抗氧化应激作用
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(攀枝花学院医学院基础整合医学教研室,四川攀枝花 617000)

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segone@163. com

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R-33

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Anti-oxidative stress effects of curcumin on rat models of traumatic brain injury
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(Department of Basic Integrated Medicine, Medical College of Panzhihua University, Panzhihua 617000, China)

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    摘要:

    目的 探讨大鼠创伤性脑损伤模型中核因子E2 转录相关因子2(Nrf2)及抗氧化应激相关因子的含量或活性的改变,研究姜黄素对大鼠脑损伤的保护作用及抗氧化应激机制?方法 选取SPF 级雄性SD 大鼠20只,分为正常对照组?脑损伤模型组(TBI 组)?脑损伤溶剂组(TBI + S 组)?脑损伤姜黄素处理组(TBI + C 组),每组各5 只?其中正常对照组仅给予麻醉和生理盐水处理,TBI 组?TBI + S 组和TBI + C 组均使用自由落体式脑外伤造模装置进行造模,随后分别给予等量生理盐水?DMSO 溶剂(0。05%)和姜黄素(5 mg/ kg)处理,1 d 后处死所有大鼠并取脑组织,提取RNA 和蛋白?定量反转录聚合酶连锁反应(qRT-PCR)检测Nrf2 的mRNA 表达,Western Blot 检测Nrf2 蛋白的表达?化学比色法检测大鼠脑组织匀浆液中丙二醛(MDA)和还原型谷胱甘肽(GSH)的含量?过氧化氢酶(CAT)和超氧化物歧化酶(SOD)的活力,酶联免疫吸附法(ELISA)检测诱导型一氧化氮合酶(iNOS)和血红素氧合酶-1(HO-1)的含量?结果< /b> 与正常对照组相比,TBI 组?TBI + S 组脑组织中Nrf2 的mRNA 和蛋白表达均显著增加,MDA 含量?iNOS 和HO-1 的活力均增加,GSH 含量?SOD 和CAT 的活性均降低,差异有显著性( P < 0.05)?与TBI 组?TBI + S 组相比,TBI + C 组Nrf2 的mRNA 和蛋白表达水平显著降低,MDA 含量?iNOS 和HO-1 的活力均降低,GSH 含量?SOD 和CAT 的活性均增加,差异有显著性( P < 0.05),而TBI 组与TBI + S 组之间差异无显著性( P < 0.05)?结论 姜黄素对脑损伤大鼠具有抗氧化应激的作用,它能够通过降低Nrf2 的表达,改变机体抗氧损伤相关指标,由此可能起到对TBI 脑组织的保护作用?

    Abstract:

    Objective To investigate the changes of content or activity of Nrf2 and anti-oxidative stress-related factors in rat models of traumatic brain injury, and explore the mechanisms of protective effect of curcumin on brain damage and oxidative stress in rats. Methods Twenty healthy SPF male SD rats were divided into 4 groups: the control group, brain injury model group (TBI group), brain injury and solvent-treated group (TBI + S group), brain injury and curcumin-treated group (TBI + C group), 5 rats ineach group. The control group received only saline and anesthesia. The TBI, TBI + S and TBI + C groups were given free falling body brain injury modeling device to establish the models and then received curcumin (5 mg/ kg), an equal amount of DMSO solvent (0.05%) and an equal amount of physiological saline, respectively. The rats were sacrificed at the next day and the RNA and proteins of brain tissues were extracted. qRT-PCR and Western blot were used to detect the mRNA and protein expression of Nrf2. Chemocolorimetry was used to detect the content or activity of MDA, GSH, CAT and SOD in the brain tissues of rats. ELISA was used to detect the contents of iNOS and HO-1. Results Compared with the control group,the mRNA and protein expressions of Nrf2, the content of MDA,the activity of HO-1 and iNOS were significantly increased, the content of GSH, the activity of SOD and CAT were significantly decreased in the TBI group and TBI + S group, with a significant difference ( P < 0.05). Compared with the TBI and TBI + S groups, the mRNA and protein expressions of Nrf2, the content of MDA, the activities of HO-1 and iNOS were significantly decreased, the content of GSH, the activity of SOD and CAT were significantly increased in the TBI + C group, showing a sigfnificant difference ( P < 0.05), but there were no significant differences between the TBI group and TBI + S group ( P < 0.05). Conclusions Curcumin has an anti?oxidative stress effect on rats with brain injury. It can reduce the expression of Nrf2, change the anti?oxidant stress?related indicators, therefore to protect the TBI?impaired brain tissues.

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梁岚,韦会平,孙妍,田金凤.姜黄素在大鼠创伤性脑损伤模型中的抗氧化应激作用[J].中国比较医学杂志,2018,28(4):73~80.

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  • 收稿日期:2017-04-18
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  • 在线发布日期: 2018-05-22
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