用于实验用猫群体遗传分析的微卫星位点筛选
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(中国食品药品检定研究院,北京 100050)

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R-33

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Selection of microsatellite loci for genetic analysis of experimental cat populations
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(National Institutes for Food and Drug Control, Beijing 100050, China)

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    摘要:

    目的 筛选适用于猫遗传质量检测的微卫星位点?方法 从相关文献和GenBank 中备选了74 个微卫星位点,摸索了最佳退火温度优化PCR 反应条件,以琼脂糖凝胶电泳以及STR 扫描结果为依据,选取条带清晰稳定?等位基因丰富?均匀分布于猫18 对常染色体上的一组微卫星位点用于实验用猫遗传质量检测?结果 从74 个备选的微卫星位点中筛选到55 个具有多态性且稳定扩增的STR 位点?并在猫的每条常染色体(除了A1?B1染色体)适当选择1 ~2 个多态性较高的STR 位点,最终得到31 个分布于18 对常染色体且等位基因多的微卫星位点?结论 成功筛选到一组适用于猫群体遗传结构分析的微卫星位点?

    Abstract:

    Objective To screen a panel of suitable microsatellite loci to analyze the genetic structure of experimental cat populations. Methods Seventy-four candidate microsatellite loci on 18 pairs of autosomes in cats were chosen from GenBank and related literature. Then, the PCR conditions were optimized by exploring the optimal annealing temperature. Based on the results of agarose gel electrophoresis and STR scanning, a set of microsatellites with clear and stable bands, abundant alleles, and even distribution on the 18 pairs of autosomes in cats were selected. Results Fiftyfive polymorphic and stably amplified STR loci were screened from 74 alternative microsatellite loci. Then, one to two higher polymorphic STR loci were selected in each autosome (other than A1 and B1 chromosomes) of the cat. Finally, 31loci with multiple alleles and even distribution on the 18 pairs of autosomes in cats were screened from 74 candidate microsatellite loci. Conclusions A set of microsatellite loci suitable for genetic structure analysis of cat populations is successfully screened.

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贾松华,王洪,魏杰,岳秉飞.用于实验用猫群体遗传分析的微卫星位点筛选[J].中国比较医学杂志,2018,28(12):55~60.

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  • 收稿日期:2018-07-02
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  • 在线发布日期: 2019-01-08
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