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贾 鹂.贞芪扶正颗粒通过 miR-200c / ZEB 通路抑制 肝癌模型大鼠血管形成[J].中国比较医学杂志,2020,30(6):31~38.
贞芪扶正颗粒通过 miR-200c / ZEB 通路抑制 肝癌模型大鼠血管形成
Zhenqi Fuzheng granules inhibit angiogenesis in rats with hepatocellular carcinoma via the miR-200c / ZEB pathway
投稿时间:2019-11-13  
DOI:10. 3969 / j.issn.1671-7856. 2020. 06. 005
中文关键词:  肝癌  贞芪扶正颗粒  血管形成  锌指蛋白  微小 RNA
英文关键词:liver cancer  Zhenqi Fuzheng granule  angiogenesis  zinc finger protein  microRNA
基金项目:
作者单位E-mail
贾 鹂 唐山职业技术学院基础医学部,唐山 063300 jlj313j@ 163.com 
摘要点击次数: 118
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中文摘要:
      目的 探究贞芪扶正颗粒对肝癌模型大鼠血管形成的抑制作用及可能的作用机制。 方法 将 Wistar 大鼠分为对照组(Control)、模型组(小剂量二乙基亚硝铵(DEN)诱导法制备)、贞芪扶正颗粒组(2. 62 g / kg)、si- miR-200c 组(20 pm)、贞芪扶正颗粒+si-miR-200c 组,每组 12 只大鼠,各组连续处理 8 周。HE 染色观察大鼠肝组 织病理形态变化;酶联免疫法检测血清中血管内皮生长因子(VEGF)、谷丙转氨酶(ALT)、谷草转氨酶(AST)水平; 荧光实时定量 PCR 法检测肝组织中 miR-200c 表达;免疫印迹法检测肝组织中锌指蛋白(ZEb-2)、VEGF 蛋白表达; 免疫组化法检测 ZEb-2、VEGF、微血管密度(MVD)阳性表达情况。 结果 与 Control 组相比,Model 组 VEGF、ALT、 AST 水平升高,肝组织中出现较多变形、坏死细胞,肝组织中 miR-200c 表达降低,ZEb-2、VEGF 蛋白表达升高,ZEb- 2、VEGF、MVD 阳性表达率升高(P< 0. 05)。与 Model 组相比,贞芪扶正颗粒组 VEGF、ALT、AST 水平降低,细胞变形程度减轻,癌细胞分化程度较高,miR-200c 表达升高,ZEb-2、VEGF 蛋白表达降低,ZEb-2、VEGF、MVD 阳性表达率降低;si-miR-200c 组 VEGF、ALT、AST 水平升高,细胞癌变程度增加,肝组织中 miR-200c 表达降低,ZEb-2、VEGF 蛋白表达升高,ZEb-2、VEGF、MVD 阳性表达率升高(P< 0. 05)。 贞芪扶正颗粒+si-miR-200c 组 VEGF、ALT、AST 水平 低于 si-miR-200c 组,ZEb-2、VEGF 蛋白表达、ZEb-2、VEGF、MVD 阳性表达率低于 si-miR-200c 组,miR-200c 表达高于 si-miR-200c 组(P< 0. 05)。 结论 贞芪扶正颗粒能够抑制肝癌模型大鼠血管形成,缓解癌症的发展,其机制可能与上调 miR-200c 表达,进而负调控 ZEb-2 蛋白表达有关。
英文摘要:
      Objective To explore the inhibitory effect of Zhenqi Fuzheng granules on angiogenesis in hepatocellular carcinoma (HCC) model rats and the possible mechanisms. Methods Wistar rats were divided into the control, model [discontinuous low-dose (Diethyl ammonium nitrite, DEN) induction method , Zhenqi Fuzheng granule (2. 62 g / kg), si-miR-200c (20 pm), and Zhenqi Fuzheng granule + si-miR-200c groups ( n= 12 rats per group) and were treated for 8 weeks. Hematoxylin and eosin staining was used to observe the pathomorphological changes in the rat liver tissues. Serum vascular endothelial growth factor ( VEGF ), alanine aminotransferase ( ALT ), and aspartate aminotransferase (AST) levels were detected via enzyme-linked immunosorbent assay ( ELISA). Real-time fluorescence quantitative PCR was used to detect miR-200c expression in the liver tissues; zinc finger protein ( ZEb-2) and VEGF protein were detected via western blot, and the positive expressions of ZEb-2, VEGF and microvessel density (MVD) were detected via immunohistochemistry. Results Compared with the control group, VEGF, ALT and AST levels were increased in the model group, and the model group livers contained more polymorphic and necrotic cells and showed decreased miR-200c expression, increased ZEb-2 and VEGF expressions, and increased ZEb-2, VEGF and MVD positivity rates (allP< 0. 05). Compared with the model group, the Zhenqi Fuzheng granule group had lower VEGF, ALT and AST levels, reduced cell deformation, higher cancer cell differentiation, increased miR-200c expression, decreased ZEb-2 and VEGF expressions, and decreased ZEb-2, VEGF and MVD positivity rates. The si-miR-200c group presented increased VEGF, ALT and AST levels, increased cell carcinogenesis, decreased miR-200c expression in the liver, increased ZEb-2 and VEGF expressions, and increased ZEb-2, VEGF and MVD positivity rates ( allP< 0. 05). VEGF, ALT and AST levels in the Zhenqi Fuzheng granule + si-miR-200c group were lower than those in the si-miR-200c group, while the ZEb- 2 and VEGF expressions and the ZEb-2, VEGF and MVD positivity rates were lower, and the miR-200c expression was higher than that of the si-miR-200c group (allP< 0. 05). Conclusions Zhenqi Fuzheng granules inhibited angiogenesis and alleviated HCC development in rats. Its mechanism may be related to upregulating miR-200c expression and downregulating ZEb-2 protein expression.
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