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刘 权.食蟹猴奇异变形杆菌的分离鉴定及药敏试验[J].中国比较医学杂志,2020,30(6):88~92.
食蟹猴奇异变形杆菌的分离鉴定及药敏试验
identification, and drug susceptibility testing of Proteus mirabilis from cynomolgus monkeys
投稿时间:2019-11-29  
DOI:10. 3969 / j.issn.1671-7856. 2020. 06. 013
中文关键词:  食蟹猴  奇异变形杆菌  分离培养  药敏试验
英文关键词:cynomolgus monkey  Proteus mirabilis  isolation culture  drug susceptibility test
基金项目:
作者单位E-mail
刘 权 中国医学科学院/ 北京协和医学院医学生物学研究所,云南省重大传染病疫苗研发重点实验室,昆明 650118 liuquan678@ imbcams.com.cn 
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中文摘要:
      目的 通过对引进食蟹猴肠道微生物检测分离到的奇异变形杆菌进行鉴定和药敏实验,为引进的实验猴微生物检测和如何开展饲养管理、疾病防控提供理论和技术支撑。 方法 肛拭子采集食蟹猴粪样,进行细 菌分离培养、生化鉴定、药敏试验和小鼠致病性试验。 结果 60 只食蟹猴中有 8 只分离到可疑细菌,经鉴定为奇异变形杆菌,表明该群体食蟹猴奇异变形杆菌感染率为 13. 3%;药敏试验结果显示该菌对头孢曲松、头孢赛肟,头孢 哌酮、头孢唑啉、青霉素等高度敏感,对阿米卡星、庆大霉素、氨苄西林钠、呋喃妥因等中度敏感,对诺氟沙星、四环 素、低度敏感、对杆菌肽、环丙沙星、卡那霉素、林可霉素和呋喃唑酮产生耐药性;小鼠致病性试验表明分离到的菌 株对小鼠有较强的致病性。 结论 食蟹猴奇异变形杆菌感染相对较高,该菌对头孢菌素类抗生素较敏感,可在临 床中作为首选药物来进行疾病的治疗。 该菌有较强的致病性,对食蟹猴群体有潜在的危害性,在平时饲养管理工 作中应引起重视。
英文摘要:
      Objective Proteus mirabilis was isolated from the intestines of introduced cynomolgus monkeys and identified and tested for drug sensitivity to provide theoretical and technical support for detecting microorganisms from introduced experimental monkeys and to conduct breeding management and disease control. Methods Fecal samples were collected from cynomolgus monkeys via anal swabs, and bacterial isolation and culturing, biochemical identification, drug sensitivity tests, and mouse pathogenicity tests were performed. Results Suspicious bacteria were isolated from 8 of 60 cynomolgus monkeys and identified as Proteus mirabilis, indicating a 13. 3% infection rate of Proteus mirabilis in these monkeys. Susceptibility tests of this bacterium showed that it was highly sensitive to ceftriaxone, cefotaxime, cefoperazone, cefazolin, and penicillin; moderately sensitive to amikacin, gentamicin, ampicillin sodium, and furantoin; sensitive to norfloxacin and tetracycline; lowly sensitive to bacitracin, ciprofloxacin, kanamycin, and lincomycin; and resistant to furazolidone. Mouse pathogenicity tests showed that the isolated strains were highly pathogenic to mice. Conclusions The Proteus mirabilis infection rate was relatively high in cynomolgus monkeys. This bacterium was sensitive to cephalosporin antibiotics; therefore, cephalosporins can be used to clinically treat infections with this bacterium. Proteus mirabilis is highly pathogenic and potentially harmful to cynomolgus monkey populations and should be considered in their normal breeding management.
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