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李贝贝.一种改良的大鼠颈静脉自主给药插管及其应用[J].中国比较医学杂志,2020,30(6):113~120.
一种改良的大鼠颈静脉自主给药插管及其应用
Improved intubation of rat jugular veins for self-administering drugs
投稿时间:2019-11-28  
DOI:10. 3969 / j.issn.1671-7856. 2020. 06. 017
中文关键词:  依赖性  颈静脉插管  自主给药
英文关键词:dependence  jugular vein intubation  self-administration
基金项目:
作者单位E-mail
李贝贝 中国医药工业研究总院上海益诺思生物技术股份有限公司,上海 201203 bbli@ ncdser.com 
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中文摘要:
      目的 介绍一种改良的大鼠颈静脉插管制作方法和颈静脉插管手术方法,并应用于一项大鼠自主 给药实验研究中,评估该插管设计的使用寿命和应用效果。 方法 对 29 只已完成食物训练的雄性 SD 大鼠用改良 的插管进行颈静脉插管手术,进行丙泊酚和可卡因的自主给药模型建立,分析术后 5 个月的动物淘汰情况。 结果 丙泊酚自主给药模型和可卡因自主给药模型均建立成功,术后动物淘汰原因主要有濒死、堵管、漏液,术后 第 1~ 5 个月,每个月与插管直接相关的淘汰率分别为 7%、7%、10%、31%和 11%,在月淘汰率在 10%以内尚能接受 的情况下,该插管组件的使用寿命预计至少 3 个月,部分插管使用寿命能够长达 5 个月甚至可能 5 个月以上。动物 濒死的原因与肝肾严重损伤、电解质紊乱和体内炎症有关。 结论 改良的用于大鼠颈静脉自主给药的插管有诸多优点,本文所设计插管组件的插管大鼠最佳使用寿命可长达 3 个月,能够满足自主给药实验的基本需求,故应用效果良好,可推广使用。
英文摘要:
      Objective To introduce an improved method for intubating the jugular vein and for jugular vein intubation surgery in rats and to apply this method to a rat self-administration experimental study to evaluate the service life and application effect of this intubation design. Methods Twenty-nine male Sprague-Dawley rats who had completed food training were intubated with a modified cannula in the jugular vein. Self-administration models of propofol and cocaine were established, and elimination from the rats was analyzed at approximately 5 months postoperation. Results Both the propofol and cocaine self-administration models were successfully established. The main reasons for postoperational drug elimination were death of the rats, blocked tubes, and fluid leakage. In months 1-5 after surgery, the intubation-related elimination rates for each month were 7%, 7%, 10%, 31%, and 11%, respectively. With an acceptable monthly elimination rate of <10%, the service life of the intubation is expected to be at least 3 months, and some can be as long as 5 months or more. Rat deaths were related to severe liver and kidney damage, electrolyte disturbances and inflammation. Conclusions Improved intubation of rat jugular veins for drug self-administration has many advantages. The optimal service life of this intubation technique was up to 3 months, which meets the basic requirements of self-administration experiments. Therefore, this application effect is good and should be promoted.
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