青蒿琥酯对阿霉素致大鼠肾损伤的保护作用
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山东省医学科学院附属医院,山东第一医科大学,济南 250031

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R-33

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Protective effect and mechanisms of artesunate on adriamycin-induced kidney injury in rats
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Affiliated Hospital of Shandong Academy of Medical Sciences,Shandong First Medical University, Jinan 250031, China

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    摘要:

    目的 观察青蒿琥酯(ART)对阿霉素致大鼠肾损伤的保护作用,并初步探讨其作用机制。 方法 32 只雄性 Wistar 大鼠,随机分为空白对照组(NC 组)、阿霉素模型组(ADR 组)、青蒿琥酯低剂量组(ART-L)、ART 高剂量组(ART-H),每组 8 只。 其中 ADR 组、ART-L 组和 ART-H 组均给予大鼠单次注射阿霉素(7. 5 mg / kg)构建 阿霉素肾损伤模型。 造模成功后,ART-L 组和 ART-H 组分别给予 25 mg / kg 和 50 mg / kg 的 ART,NC 组给予等体积的生理盐水。 连续灌胃 3 周后,全自动生化分析仪检测各组大鼠血清中白蛋白(ALB)、胆固醇(CHOL)、甘油三酯 (TG)和肾功能指标血尿素氮(BUN)和血肌酐( Scr)的含量; HE 染色观察各组大鼠肾组织病理学改变;使用试剂盒检测各组大鼠肾组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性以及丙二醛(MDA)含量。 结果 与 NC 组相比,ADR 组大鼠肾功能指标血清 BUN、血清 Scr 和肾组织 MDA 水平明显升高(均 P<0. 01),血清 ALB 和肾组织 SOD、GSH-Px 活力下降(均 P<0. 01)。 与 ADR 组相比,ART-L 组和 ART-H 组大鼠肾功能指标血清 BUN、血清 Scr 和肾组织 MDA 水平明显降低(均 P< 0. 01),血清 ALB 和肾组织 SOD、GSH-Px 活力升高(均 P< 0. 01)。 HE 染色显示 ART 可减轻 ADR 致大鼠肾损伤程度。 结论 ART 灌胃可以降低 ADR 致肾损伤大鼠的蛋白 尿,改善肾功能和肾组织病理损伤,这种作用可能是通过青蒿素抑制肾组织氧化应激实现的。

    Abstract:

    Objective To investigate and explore the potential mechanism of the protective effects of artesunate on adriamycin-induced kidney injury in rats. Methods Thirty-two male Wistar rats were randomly divided into four groups of 8 rats each: negative control ( NC), kidney injury model ( ADR), low-dose artesunate ( ART-L), and high-dose artesunate (ART-H). The model group, ART-L and ART-H treatment groups received a single tail vein injection of adriamycin (7. 5 mg / kg body weight).After modeling, the rats in the treatment groups were given 25 mg / kg (ART-L) and 50 mg / kg (ART-H) artesunate, intragastrically for 3 consecutive weeks. The NC and ADR groups were injected with isotonic saline. Blood urea nitrogen ( BUN) and serum creatinine ( Scr) were measured by an automatic biochemical analyzer. Hematoxylin and eosin ( HE) staining was used to observed renal morphological changes. Kits were used to measure the levels of superoxide dismutase ( SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) in renal tissues of rats in each group. Results Serum levels of BUN and Scr, and the content of MDA in renal tissues were significantly higher in the ADR group than the NC group (P< 0. 01), while the serum levels of ALB and the activities of SOD and GSH-Px in renal tissues were significantly lower (P< 0. 01). Compared with the ADR group, serum levels of BUN and Scr, and the content of MDA in renal tissues were significantly lower in the ART-L and ART-H groups, while the serum levels of ALB and the activities of SOD and GSH-Px in renal tissues were significantly higher ( P< 0. 01). HE staining revealed that artesunate reduced renal injury induced by adriamycin. Conclusions Artesunate reduced urine protein in rats with adriamycin-induced kidney injury, improving renal function and pathological tissue damage. The underlying mechanism may be related to the inhibition of oxidative stress induced by adriamycin in renal tissue.

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陶 磊,李婷婷,马旖旎,陈仁兵,李雪芹,王金娜.青蒿琥酯对阿霉素致大鼠肾损伤的保护作用[J].中国比较医学杂志,2020,30(11):47~51.

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  • 收稿日期:2020-09-02
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  • 在线发布日期: 2020-12-25
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