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秦艳萍,李晓龙,李慧茹,陆春果,杨 粤,张钧雄.α-倒捻子素对免疫抑制小鼠免疫功能的影响[J].中国比较医学杂志,2021,31(5):65~71,94.
α-倒捻子素对免疫抑制小鼠免疫功能的影响
The effect of α-mangostin on immune function in immunosuppressed mice
投稿时间:2020-07-26  
DOI:10. 3969 / j.issn.1671-7856. 2021. 05. 011
中文关键词:  α-倒捻子素  免疫抑制小鼠  免疫功能
英文关键词:α-mangostin  immunosuppressive mice  immune function
基金项目:
作者单位E-mail
秦艳萍 广西医科大学,南宁 530021 1278415620@ qq.com 
李晓龙 广西医科大学,南宁 530021 xlongli@ outlook.com 
李慧茹 广西医科大学,南宁 530021  
陆春果 广西医科大学,南宁 530021  
杨 粤 广西医科大学,南宁 530021  
张钧雄 广西医科大学,南宁 530021  
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中文摘要:
       目的 探讨 α-倒捻子素对环磷酰胺致小鼠免疫抑制模型免疫功能的影响,为后续的机制研究及开发增加免疫功能的新药提供实验依据。 方法 将小鼠随机分成 5 组,每组 10 只:空白对照组、免疫抑制组、α-倒捻子素高剂量组、中剂量组和低剂量组。采用灌胃给药的方法,前 7 d,除空白对照组外,其他 4 组给予环磷酰胺 (CTX)创建免疫低下模型;后 14 d,空白对照组继续灌胃生理盐水,免疫抑制组灌胃玉米油,三个剂量组分别灌胃不同剂量的 α-倒捻子素。末次给药后 24 h 处死小鼠,进行迟发型变态反应(DTH)检测、血清溶血素测定(HC50 )、 巨噬细胞吞噬功能的测定(碳廓清实验)及免疫器官胸腺和脾指数测定、自然杀伤细胞(NK 细胞)活性测定(MTT 法)、外周血白细胞计数的测定,以及脾淋巴细胞增殖实验,研究 α-倒捻子素对小鼠免疫功能的影响。 结果 小鼠免疫抑制模型创建成功。灌胃 α-倒捻子素后,三个剂量组能不同程度提高免疫抑制小鼠胸腺和脾指数、半数溶血值、脾淋巴细胞增殖率、NK 细胞活性,其中高剂量组,总 α-倒捻子素灌胃剂量为 100 mg / ( kg·d),其效果最为突出, 在多个实验中,与免疫抑制组相比,差异极显著,具有统计学意义(P<0. 01);而在迟发型变态反应中,除高剂量组与空白对照组及免疫抑制组比较具有显著差异(P<0. 05),其他剂量组均无统计学意义(P>0. 05);在外周血白细胞计数测定实验中,高剂量组与免疫抑制组比较具有极显著的差异(P<0. 01),其他组均无统计学意义(P>0. 05);在碳廓清实验中,各剂量组与空白对照组、免疫抑制组比较均无统计学意义(P>0. 05)。 结论 倒捻子素对免疫抑制小鼠的免疫功能具有调节作用,其对免疫抑制小鼠免疫功能改善的最适宜剂量更偏向于高剂量组,在一定范围内具有剂量依赖性。
英文摘要:
       Objective To investigate the effect of α-mangostin on immune function in a cyclophosphamide (CTX)-induced immunosuppressive mouse model, so as to provide experimental basis for further study of the mechanism of α-mangostin and its potential for development as a new drug to enhance immunity. Methods Mice were randomly divided into five groups: normal control group, immunosuppressive group, and high, middle, and low dose groups. All groups except the normal control group were given CTX to create the immunosuppressive model in the first 7 days. In the latter 14 days, the normal control group was given normal saline, the immunosuppressive group was given corn oil, and the three dose groups were given different doses of α-mangostin. The mice were killed 24 h after the last treatment. We assessed the effect of α-mangostin on immune function in mice by detecting delayed allergic reaction ( DTH), serum hemolysin ( HC50), macrophage phagocytosis (carbon clearance test), thymus and spleen index of immune organs, natural killer cell (NK cell) activity (MTT method ), peripheral white blood cell count, and splenic lymphocyte proliferation. Results The immunosuppressive mouse model was established by intragastric administration of CTX. After administration, the thymus and spleen index, hemolytic value, spleen lymphocyte proliferation rate and NK cell activity in immunosuppressive mice were increased by different doses of α-mangostin. The effect was most prominent in the high dose group, in which the total dose of α-mangostin was [100 mg (kg,d)-1 ]. In many experiments, the difference was statistically significant compared with the immunosuppressive group (P< 0. 01). In the delayed allergic reaction, there was significant difference between the high dose group and the normal control group and immunosuppressive group (P< 0. 05), but there was no significant difference between the other groups (P> 0. 05). There was a significant difference in peripheral white blood cell counts between the high dose group and the immunosuppressive group (P< 0. 01), but no conspicuous difference between the other groups ( P> 0. 05 ). Macrophage phagocytosis, measured by carbon clearance assay, was similar in the immunosuppressive group and each dose group ( P> 0. 05). Conclusions mangostin had a dose-dependent regulating effect in immunosuppressed mice, and the high dose of α-mangostin demonstrated the strongest improvement of immune function in immunosuppressed mice.
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