• 首页
  • 期刊介绍
  • 编委会
  • 投稿指南
  • 期刊订阅
  • 广告合作
  • 留言板
  • 联系我们
  • English
李 凡,张学林,冯静茹,郝 洁,周 蕾.四君子汤含药血清通过抑制 RhoA/ Rac1 / Cdc42 通路影响卵巢癌细胞的上皮间质转化[J].中国比较医学杂志,2021,31(8):29~37.
四君子汤含药血清通过抑制 RhoA/ Rac1 / Cdc42 通路影响卵巢癌细胞的上皮间质转化
Sijunzi Decoction containing serum affects the epithelial mesenchymal transition of ovarian cancer cells by inhibiting the RhoA / Rac1 / Cdc42 pathway
投稿时间:2020-07-03  
DOI:10. 3969 / j.issn.1671-7856. 2021. 08. 005
中文关键词:  四君子汤含药血清  人卵巢癌细胞  Ras 同源基因家族成员 A  Ras 相关的 C3 肉毒素底物 1  细胞分裂周期蛋白 42  上皮间质化
英文关键词:Sijunzi Decoction containing serum  human ovarian cancer cell  Ras homolog gene family member A  Ras-related C3 botulinum toxin substrate 1  cell division cycle 42  epithelial mesenchymal
基金项目:
作者单位E-mail
李 凡 河北北方学院附属第二医院,张家口 075100 lifanfan492@ 163.com 
张学林 河北北方学院附属第二医院,张家口 075100  
冯静茹 河北北方学院附属第二医院,张家口 075100  
郝 洁 河北北方学院附属第二医院,张家口 075100  
周 蕾 河北北方学院附属第二医院,张家口 075100  
摘要点击次数: 196
全文下载次数: 84
中文摘要:
       目的 探索四君子汤含药血清对卵巢癌细胞增殖迁移、侵袭及上皮间质化的影响,并初步研究其作用机理。 方法 体外培养人卵巢癌细胞(SKOV3)和人卵巢上皮细胞,随机分为空白对照组、舒尼替尼组、四君子汤 I 组、II 组和 III 组。噻唑蓝法检测各组 SKOV3 细胞和人卵巢上皮细胞增殖变化情况;划痕实验和侵袭( transwell) 实验检测各组 SKOV3 细胞迁移和侵袭能力;蛋白免疫印迹分析法检测各组 SKOV3 细胞上皮型钙粘蛋白( E- cadherin)、神经性钙黏附蛋白(N-cadherin)、波形蛋白(Vimentin)、Ras 同源基因家族成员 A(RhoA)、Ras 相关的 C3 肉毒素底物 1(Rac1)和细胞分裂周期蛋白 42(Cdc42)蛋白表达情况;实时荧光定量 PCR( qRT-PCR)法检测各组 SKOV3 细胞 RhoA、Rac1 和 Cdc42 mRNA 表达变化。 结果 各组人卵巢上皮细胞存活率差异无统计学意义(P> 0. 05)。 与空白对照组相比,舒尼替尼组 SKOV3 细胞存活率、划痕愈合率、侵袭数量、N-cadherin、Vimentin 蛋白、 RhoA、Rac1、Cdc42 mRNA 和蛋白表达显著降低(P<0. 05),E-cadherin 蛋白表达显著升高(P<0. 05)。 四君子汤 I、 II、III 组 SKOV3 细胞存活率、划痕愈合率、侵袭数量、N-cadherin、Vimentin 蛋白、RhoA、Rac1、Cdc42 mRNA 和蛋白表达依次降低(P<0. 05),E-cadherin 蛋白表达依次升高(P<0. 05),呈剂量依赖性。 四君子汤 III 组上述指标和舒尼替尼组比较差异无统计学意义(P>0. 05)。 结论 四君子汤含药血清能够抑制人卵巢癌细胞增殖、迁移、侵袭和上皮间质化,可能与 RhoA/ Rac1 / Cdc42 信号通路激活受阻相关。
英文摘要:
       Objective To explore the effects of Sijunzi Decoction containing serum on the proliferation, migration, invasion, and epithelial mesenchymal transition of ovarian cancer cells, and the mechanism involved. Methods Human ovarian cancer cells (SKOV3) and human ovarian epithelial cells were cultured in vitro and randomly divided into blank control, sunitinib, and Sijunzi Decoction I, II, and III groups. The methyl thiazolyl tetrazolium assay was used to detect the proliferation of SKOV3 cells and human ovarian epithelial cells. The migration and invasion abilities of SKOV3 cells were examined using the scratch test and transwell test. The protein expressions of E-cadherin, N-cadherin, vimentin, RAS homolog gene family member A (RhoA), Ras-related C3 botulinum toxin substrate 1 (Rac1), and cell division cycle 42 (Cdc42) in SKOV3 cells were detected by western blotting. In addition, the mRNA expressions of RhoA, Rac1, and Cdc42 in SKOV3 cells were detected by real-time quantitative PCR ( qRT-PCR). Results There was no significant difference in the survival rate of human ovarian epithelial cells among the groups (P> 0. 05). Compared with the blank control group, the survival rate, wound healing rate, invasion number, protein expressions of N-cadherin and vimentin, and mRNA and protein expressions of RhoA, Rac1, and Cdc42 in SKOV3 cells in the sunitinib group were significantly lower (P<0. 05), and the protein expression of E-cadherin was significantly higher (P<0. 05). The survival rate, wound healing rate, invasion number, protein expressions of N-cadherin and vimentin, and mRNA and protein expressions of RhoA, Rac1, and Cdc42 in SKOV3 cells in the Sijunzi Decoction I, II, and III groups were decreased (P<0. 05), and the protein expression of E-cadherin was increased (P< 0. 05) dose-dependently. There was no significant difference in these indexes between the Sijunzi Decoction III and sunitinib groups ( P> 0. 05 ). Conclusions Sijunzi Decoction containing serum inhibits the proliferation, migration, invasion, and epithelial mesenchymal transition of human ovarian cancer cells, which may be related to inhibition of the RhoA/ Rac1 / Cdc42 signaling pathway activation.
查看全文  查看/发表评论  下载PDF阅读器
关闭
您是第 4911227 位访问者
版权所有:中国实验动物学会 主管单位:中国科学技术协会 主办单位:中国实验动物学会 中国医学科学院医学实验动物研究所
地  址: 北京市朝阳区潘家园南里5号 邮编:100021 电话:010-67779337 E-mail:bjb@cnilas.org
本系统由北京勤云科技发展有限公司设计
微信关注二维码