肺转移灶源性 Lewis 肺癌在 C57BL / 6 小鼠体内生长及转移特性的研究
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1.河南中医药大学医学院,郑州 450046;2.河南中医药大学中医药科学院,郑州 450046;3.河南中医药大学药学院,郑州 450046

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R-33

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Study on the growth and metastasis characteristics of Lewis lung cancer derived from lung metastases in C57BL/ 6 mice
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1.College of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China.2. Academy of Traditional Chinese Medicine, Henan University of Chinese Medicine, Zhengzhou 450046.3. College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046

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    摘要:

    目的 研究肺转移灶源性 Lewis 肺癌在 C57BL/ 6 小鼠体内成瘤及转移情况。 方法 取发生肺转移的 Lewis 肺癌荷瘤小鼠,解剖并分离肺转移灶制成细胞悬液,右前肢腋窝皮下接种于 C57BL/ 6 小鼠体内,制备肺转移灶源性 Lewis 肺癌小鼠模型,记为肺瘤组。 同时设皮下移植瘤传代小鼠,记为皮下组。定期测量各组小鼠肿瘤直径,计算肿瘤体积;绘制生存曲线;解剖观察肺和肝的病变;HE 染色检测肺和肝的病理变化;透射电镜观察肿瘤细胞的超微结构。 结果 肺瘤组小鼠肿瘤体积小于皮下移植瘤组(P>0. 05);观察期内肺瘤组小鼠存活率较皮下组高;解剖可见肺瘤组肺转移率为 37. 5%,肝转移率为 25. 0%,皮下组肺转移率为 20. 0%,无肝转移;HE 染色显示肺瘤组肺和肝转移灶体积大,染色深,外观近似圆形,与周围组织分界明显,皮下组肺转移灶体积小,无典型肝转移;透射电镜下可见转移灶内肿瘤细胞出现典型的奇异型核和病理性核分裂象。 结论 肺瘤源性小鼠较皮下移植瘤小鼠肿瘤生长缓慢,在相同观察期内存活率更高,在小鼠体内表现出更强的的远处转移特性。

    Abstract:

    Objective To study the tumorigenesis and metastasis of Lewis lung cancer derived from lung metastases in C57BL/ 6 mice. Methods Lewis lung cancer tumor-bearing mice with lung metastasis were dissected, and lung metastases were isolated to prepare a cell suspension, which was inoculated subcutaneously into the axilla of the right forelimbs of C57BL/ 6 mice. A mouse model of Lewis lung cancer derived from lung metastases was established and designated as the lung tumor group. Subcutaneously transplanted tumor passaged mice were used as the subcutaneous group. The tumor diameter of mice was measured periodically, the tumor volume was calculated, and a survival curve was drawn. Anatomical observation was performed on lung and liver lesions, and HE staining was used to detect pathological changes in the lungs and liver. The ultrastructure of tumor cells was observed by transmission electron microscopy. Results The tumor volume of the lung tumor group was less than that of the subcutaneous group(P>0. 05). The survival rate of the lung tumor group was higher than that of the subcutaneous group during the observation period. Anatomic observation showed the lung and liver metastasis rates to be 37. 5% and 25% in the lung tumor group and 20% and no liver metostasis in the subcutaneous group, respectively. In HE staining, lung and liver metastases in the lung tumor group were large,darkly stained, approximately round in appearance, and demarcation from surrounding tissues was obvious. Lung metastases in subcutaneous group were small, and no typical liver metastases were seen. Tumor cells in the metastases showed typical bizarre nuclei and pathological mitosis under transmission electron microscopy. Conclusions Compared with subcutaneous tumor mice, lung tumor mice showed slower tumor growth, a higher survival rate within the same observation period, and stronger distant metastatic properties.

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张 娜,刘学芳,冯妍妍,郭凤悦,冯素香.肺转移灶源性 Lewis 肺癌在 C57BL / 6 小鼠体内生长及转移特性的研究[J].中国比较医学杂志,2022,32(11):12~17.

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  • 收稿日期:2022-04-08
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  • 在线发布日期: 2023-01-18
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